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The Signal Transduction System in Luteotrophic Stimulation of Expression of the 3β-HSD Gene in Porcine Granulosa Cells in Culture

  • P. Jorge Chedrese
  • Daniel Schott
  • David Zhang
  • Bruce D. Murphy
Part of the Serono Symposia USA book series (SERONOSYMP)

Abstract

The enzyme 3β-hydroxy-\( \Delta \) 5-steroid dehydrogenase (3β-HSD) catalyses the formation of \( \Delta \) 4-3ketosteroids from \( \Delta \) 5-ene, 3β-hydroxysteroids (1). Because it converts pregnenolone to progesterone, it is central to the production of steroid hormones. In rat granulosa cells, follicle stimulating hormone (FSH) and human chorionic gonadotropin (hCG), which stimulate steroidogenesis, increase the velocity of 3β-HSD, while gonadotrophin releasing hormone, which inhibits steroidogenesis, reduces the velocity, but the Km is unaffected by these hormones (2, 3). In this chapter, we discuss evidence that in luteinized cultured pig granulosa cells, luteotrophins and cAMP induce an increase in 3β-HSD mRNA levels that can be inhibited by the activation of protein kinase C.

Keywords

Granulosa Cell Signal Transduction System Luteal Tissue Porcine Granulosa Cell Dibutyryl Adenosine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York, Inc. 1991

Authors and Affiliations

  • P. Jorge Chedrese
  • Daniel Schott
  • David Zhang
  • Bruce D. Murphy

There are no affiliations available

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