Abstract
The enzyme 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase (3β-HSD) catalyzes the transformation of Δ5-ene, 3β-hydroxy to Δ4-3-keto steroids and is therefore an essential enzyme in the biosynthesis of all steroid hormones. 3β-HSD deficiency results in decreased formation of cortisol, aldosterone, and sex steroids (1). Clinically, 3β-HSD deficiency causes ambiguous genitalia and lethal adrenal insufficiency in newborns (2). On the other hand, the reversible interconversion of Δ5-androstenediol to DHEA, testosterone to Δ4-androstenedione, and estrone to estradiol is mediated by 17β-hydroxysteroid dehydrogenase (17β-HSD), also termed 17-oxidoreductase or 17-ketosteroid reductase. Deficient activity of ovarian 17β-HSD can cause hirsutism and polycystic ovarian disease (3). 3β-HSD and 17β-HSD are both non-cytochrome P-450 enzymes using NAD and NADPH as cofactors, respectively.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bongiovani AM. Congenital adrenal hyperplasia due to 3β-HSD deficiency. In: New MI, Levine LS, eds.; vol 13. Basel: Karger, 1984: 72 – 82.
White PC, New MI, Dupont B. Congenital adrenal hyperplasia. N Engl J Med 1987; 316: 1519 – 24.
Pang S, Softness B, Sweeney WJ, New MI. N Engl J Med 1987; 316: 1295 – 1301.
Luu-The V, Lachance Y, Labrie C, et al. Full length cDNA structure and deduced amino acid sequence of human 3P-hydroxy-5-ene steroid dehydrogenase. Mol Endocrinol 1989; 3: 1310 – 2.
Luu-The V, Labrie C, Zhao HF, et al. Characterization of cDNAs for human estradiol 17β-dehydrogenase and assignment of the gene to chromosome 17. Mol Endocrinol 1989; 3: 1301 – 9.
Golos TG, Miller ML, Strauss JF, III. Human chorionic gonadotropin and 8 BrcAMP promote an acute increase in cytochrome P450scc and adrenodoxin mRNAs in cultured human granulosa cells by a cycloheximide-insensitive mechanism. J Clin Invest 1987; 80: 896 – 9.
Chedrese PJ, Rajkumar K, Murphy BD. Dose response of luteinized porcine granulosa cells in vitro to prolactin: Dependency on exposure to human chorionic gonadotrophin. Can J Physiol Pharmacol 1988; 66: 1337 – 40.
Cathala G, Savouret JF, Mendez B, et al. A method for isolation of intact translationally active RNA. DNA 1983; 2: 329 – 33.
Feinberg AP, Vogelstein B. A technique for radiolabelling DNA restriction endonuclease fragments. Anal Biochem 1983: 132: 6 – 13.
Tremblay Y, Ringler GE, Morel Y, et al. Regulation of the gene for estrogenic 17-Ketosteroid reductase lying on chromosome 17. J Biol Chem 1989; 264: 20459 – 62.
Erickson GF, Magoffin DA, Dyer CA, Hofeditz C. The ovarian androgen pro-ducing cells: A review of structure and function. Endocr Rev 1985; 6: 371 – 99.
Voutilainen R, Tapanainen J, Chung BC, Matteson KJ, Miller WL. Hormonal regulation of P450scc and P450cl7 in cultured human granulosa cells. J Clin Endocrinol Metab 1986; 63: 202 – 6.
Fevold HL. Synergism of follicle stimulating and luteinising hormone in producing estrogen secretion. Endocrinology 1941; 28: 33 – 9.
Greep RO, Van Dyke HB, Ghow BF. Gonadotropins of the swine pituitary. Endocrinology 1942; 30: 63 – 9.
Hsueh AJW, Adashi EY, Jones PBC, Welsh TH. Hormonal regulation of differentiation of cultured varian granulosa cells. Endocr Rev 1984; 5: 76 – 127.
Wang C, Hsueh AJW, Erickson GF. Induction of functional PRL receptors by FSH in rat granulosa cells in vivo and in vitro. J Biol Chem 1979; 254: 11330 – 5.
Jones PBC, Valk CA, Hsueh AJW. Regulation of progestin biosynthetic enzymes in culture rat granulosa cells. Biol Reprod 1981; 29: 572 – 8.
Veldhuis JD, Klase P, Hammond JM. Divergent effects of prolactin upon steroidogenesis by porcine granulosa cells in vitro. Endocrinology 1981; 107: 42 – 9.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer-Verlag New York, Inc.
About this chapter
Cite this chapter
Tremblay, Y., Marcotte, B., Strauss, J.F. (1991). Differential Regulation of 3β-HSD and 17β-HSD Expression in Granulosa Cells. In: Gibori, G. (eds) Signaling Mechanisms and Gene Expression in the Ovary. Serono Symposia USA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-3200-1_26
Download citation
DOI: https://doi.org/10.1007/978-1-4612-3200-1_26
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4612-7830-6
Online ISBN: 978-1-4612-3200-1
eBook Packages: Springer Book Archive