Localization of mRNAs That Encode Steroidogenic Enzymes in Bovine Ovaries

  • R. J. Rodgers
  • H. F. Rodgers
Part of the Serono Symposia USA book series (SERONOSYMP)


Immunocytochemical localization of the steroidogenic enzymes in bovine follicles and corpora lutea (1, 2) allowed the identification of the steroidogenic properties of ovarian cells in situ. To further extend this work, we present here the localization of cholesterol side-chain cleavage cytochrome P-450 (P-450SCC) mRNAs and of 17α-hydroxylase (P-45017α) mRNAs in bovine ovaries by in situ hybridization (J. Reprod. Fertil., submitted) and compare these results with that of the immunolocalization of the enzymes they encode (1, 2). These enzymes convert cholesterol into pregnenolone and then into androgen, respectively.


Corpus Luteum Luteal Cell Steroidogenic Enzyme Bovine Corpus Luteum Bovine Ovary 
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  1. 1.
    Rodgers RJ, Rodgers HF, Hall PF, Waterman MR, Simpson ER. Immuno- localization of cholesterol side-chain-cleavage cytochrome P–450 and 17 α- hydroxylase cytochrome P–450 in bovine ovarian follicles. J Reprod Fertil 1986; 78: 627 – 38.PubMedCrossRefGoogle Scholar
  2. 2.
    Rodgers RJ, Rodgers HF, Waterman MR, Simpson ER. Immunolocalization of cholesterol side-chain cleavage cytochrome P–450 and ultrastructural studies of bovine corpora lutea. J Reprod Fertil 1986; 78: 639 – 52.PubMedCrossRefGoogle Scholar
  3. 3.
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  4. 4.
    Rodgers RJ, Waterman MR, Simpson ER. Levels of messenger ribonucleic acid encoding cholesterol side-chain cleavage cytochrome P–450, 17-hydroxylase cytochrome P–450, adrenodoxin, and low density lipoprotein receptor in bovine follicles and corpora lutea throughout the ovarian cycle. Mol Endocrinol 1987; 1: 274 – 9.PubMedCrossRefGoogle Scholar
  5. 5.
    Rodgers RJ, Waterman MR, Simpson ER. Cytochromes P-450scc, P-45017α, adrenodoxin and reduced nicotinamide andenine dinucleotide phosphate-cyto- chrome P–450 reductase in bovine follicles and corpora lutea. Changes in specific contents during the ovarian cycle. Endocrinology 1986; 118: 1366 – 74.PubMedCrossRefGoogle Scholar
  6. 6.
    Rodgers RJ. Steroidogenic cytochrome P–450 enzymes and ovarian steroidogenesis. Reprod Fertil Dev 1990; 2: 153 – 63.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1991

Authors and Affiliations

  • R. J. Rodgers
  • H. F. Rodgers

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