Localization of mRNAs That Encode Steroidogenic Enzymes in Bovine Ovaries
Immunocytochemical localization of the steroidogenic enzymes in bovine follicles and corpora lutea (1, 2) allowed the identification of the steroidogenic properties of ovarian cells in situ. To further extend this work, we present here the localization of cholesterol side-chain cleavage cytochrome P-450 (P-450SCC) mRNAs and of 17α-hydroxylase (P-45017α) mRNAs in bovine ovaries by in situ hybridization (J. Reprod. Fertil., submitted) and compare these results with that of the immunolocalization of the enzymes they encode (1, 2). These enzymes convert cholesterol into pregnenolone and then into androgen, respectively.
KeywordsCholesterol Albumin Chrome EDTA Androgen
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