Advertisement

State of the Art and Future Directions in Human IVF

  • Alan Trounson
Conference paper
Part of the Serono Symposia, USA book series (SERONOSYMP)

Abstract

The techniques for human in vitro fertilization (IVF) by Edwards and Steptoe in the 1970s (1, 2) and Wood and colleagues in the late 1970s and early 1980s (3–5) have been developed into a broad spectrum of treatment strategies that are now known as the new reproductive technologies for assisted human conception. The complex nature of the treatment options that now exist is often not appreciated, and there is frequently a misunderstanding of the interrelationships between the various options and their application. This chapter identifies the treatment options and their relationships to one another.

Keywords

Embryo Transfer Human Oocyte Oocyte Donation Embryo Viability Male Factor Infertility 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Edwards RG, Steptoe PC, Purdy JM. Establishing full-term human pregnancies using cleaving embryos grown in vitro. Br J Obstet Gynaecol 1980;87:737–756.PubMedCrossRefGoogle Scholar
  2. 2.
    Steptoe PC, Edwards RG, Purdy JM. Clinical aspects of pregnancies established with cleaving embryos grown in vitro. Br J Obstet Gynaecol 1980;87:757–768.PubMedCrossRefGoogle Scholar
  3. 3.
    Lopata A, Johnston IWH, Hoult IJ, Speirs AI. Pregnancy following intrauterine implantation of an embryo obtained by in vitro fertilization of a preovulatory egg. Fertil Steril 1980;33:117–20.PubMedGoogle Scholar
  4. 4.
    Trounson AO, Leeton JF, Wood C, Webb J, Wood J. Pregnancies in humans by fertilization in vitro and embryo transfer in the controlled ovulatory cycle. Science 1981;212:681–682.PubMedCrossRefGoogle Scholar
  5. 5.
    Trounson AO, Mohr LR, Wood C, Leeton JF. Effect of delayed insemination on in vitro fertilization, culture and transfer of human embryos. J Reprod Fertil 1982;64:285–294.PubMedCrossRefGoogle Scholar
  6. 6.
    Cameron IT, O’Shea FC, Rolland JM, Hughes EG, deKretser DM, Healy DL. Occult ovarian failure:a syndrome of infertility, regular menses and elevated follicle-stimulating hormone concentrations. J Clin Endocrinol Metab 1988;67(6): 1190–1194. PubMedCrossRefGoogle Scholar
  7. 7.
    Mcintosh JEA, Matthews CD, Crocker JM, Broom TJ, Cox LW. Predicting the luteinizing hormone surge: relationship between the duration of the follicular and the luteal phases and the length of the human menstrual cycle. Fertil Steril 1980;34:125–130.PubMedGoogle Scholar
  8. 8.
    Foulot H, Ranoux C, Dubuisson J-B, Rambaud D, Aubriot F-X, Poirot C. In vitro fertilization without ovarian stimulation: a simplified protocol applied in 80 cycles. Fertil Steril 1989;52:617–621.PubMedGoogle Scholar
  9. 9.
    Trounson A, Hammarberg K. The benefits of natural cycle IVF. Healthright 1989;8:22–25.Google Scholar
  10. 10.
    McBain JC, Trounson A. Patient management-treatment cycle. In: Wood C, Trounson A, eds. Clinical in vitro fertilization. Berlin: Springer-Verlag, 1984:49–65.Google Scholar
  11. 11.
    Trounson AO, Calabrese R. Changes in plasma progesterone concentrations around the time of the luteinizing hormone surge in women superovulated for in vitro fertilization. J Clin Endocrinol Metab 1984;59:1075–1080.PubMedCrossRefGoogle Scholar
  12. 12.
    Jones HW. In vitro fertilization. In: Behrman SJ, Kistner RW, Patton GW, eds. Progress in infertility. 3rd ed. Boston: Little Brown, 1988:534–561.Google Scholar
  13. 13.
    MacLachlan V, Besanko M, O’Shea F, Wade H, Wood C, Trounson A, Healy DL. A controlled study of luteinizing hormone-releasing hormone agonist (Buserelin) for the induction of folliculogenesis before in vitro fertilization. N Engl J Med 1989;320:1233–1237.PubMedCrossRefGoogle Scholar
  14. 14.
    MacLachlan V, Besanko M, Wade H, Morrow L, O’Shea F, Trounson A, Healy D. Luteinizing-hormone-releasing hormone agonist treatment in patients with previously failed folliculogenesis during in vitro fertilization therapy. In: Jones HW, Schrader C, eds. Fertilization and other assisted reproduction. Ann N Y Acad Sci 1988;541:60–74. PubMedCrossRefGoogle Scholar
  15. 15.
    Poison DW, MacLachlan V, Krapez JA, Wood C, Healy DL. A controlled study of gonadotrophin-releasing hormone agonist (Buserelin acetate) for folliculargenesis in routine in vitro fertilization patients. Fertil Steril 1991;56:509–514. Google Scholar
  16. 16.
    Winslow KL, Oehninger SC, Toner JP, Acosta AA, Brzyski RG, Muasher SJ. The gonadotrophin-releasing hormone agonist stimulation test — a sensitive predicter of performance in the flare-up in vitro fertilization cycle. Fertil Steril 1991;56:711–717.PubMedGoogle Scholar
  17. 17.
    Dirnfeld M, Gonen Y, Lissak A, et al. A randomised prospective study on the effect of short and long Buserelin treatment in women with repeated unsuccessful in vitro fertilization (IVF) cycles due to inadequate ovarian response. J In Vitro Fertil Embryo Transfer 1991;8:339–343.CrossRefGoogle Scholar
  18. 18.
    Pellicer A, Lightman A, Diamond MP, Russell JB, DeCherney AH. Outcome of in vitro fertilization in women with low response to ovarian stimulation. Fertil Steril 1987;47:812–815.PubMedGoogle Scholar
  19. 19.
    Owen EJ, West C, Mason BA, Jacobs HS. Co-treatment with growth hormone of sub-optimal responders in IVF-ET. Hum Reprod 1991;6:524–528.PubMedGoogle Scholar
  20. 20.
    Forman RG, Demouzon J, Feinstein MC, Testart J, Frydman R. Studies on the influence of gonadtrophin levels in the early follicular phase on the ovarian response to stimulation. Hum Reprod 1991;6:113–117.PubMedGoogle Scholar
  21. 21.
    Leeton J, Rogers P, Cameron I, Caro C, Healy D. Pregnancy results following embryo transfer in women receiving low-dosage variable-length estrogen replacement therapy for premature ovarian failure. J In Vitro Fertil Embryo Transfer 1989;6:232–235.CrossRefGoogle Scholar
  22. 22.
    Trounson A. The production of ruminant embryos in vitro. Anim Reprod Sci 1992.Google Scholar
  23. 23.
    Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from non-stimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril 1991;55:109–113.PubMedGoogle Scholar
  24. 24.
    Osborn JC, Trounson A. Unpublished data.Google Scholar
  25. 25.
    Osborn JC. Oocyte retrieval and maturation. In:Trounson AO, Gardner DG, eds. Handbook of in vitro fertilization. CRC Press, 1992. Google Scholar
  26. 26.
    Bollen N, Tournaye H, Camus M, Devroey P, Staessen C, Van Steirteghem AC. The incidence of multiple pregnancy after in vitro fertilization and embryo transfer, gamete, or zygote intrafallopian transfer. Fertil Steril 1991;55:314–318.PubMedGoogle Scholar
  27. 27.
    Toth TL, Brzyski RG, Oehninger S, Acosta AA, Toner JP, Muasher SJ. Embryo transfer to the uterus or the fallopian tube after in vitro fertilization yields similar results. Fertil Steril 1992;57:1110–1113.PubMedGoogle Scholar
  28. 28.
    Balmaceda JP, Ord T, Alam V, Snell K, Roszjtein D, Asch RH. Embryo implantation rates in oocyte donation: a prospective comparison of tubal versus uterine transfers. Fertil Steril 1992;57:362–325.PubMedGoogle Scholar
  29. 29.
    Quinn P, Kerin JF, Warnes GM. Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 1985;44:493–498.PubMedGoogle Scholar
  30. 30.
    Gianaroli L, Seracchioli R, Ferraretti AP, Trounson A, Flamigni C, Bovicelli L. The successful use of human amniotic fluid for mouse embryo culture and human in vitro fertilization, embryo culture and embryo transfer. Fertil Steril 1986;46:907–913.PubMedGoogle Scholar
  31. 31.
    Cummins JM, Breen TM, Fuller SM, et al. Comparison of two media in a human in vitro fertilization program: lack of significant difference in pregnancy rate. J In Vitro Fertil Embryo Transfer 1986;3:326–329.CrossRefGoogle Scholar
  32. 32.
    Gianaroli L, Trounson A, King C, Farraretti A, Chiappazzo L, Bafaro G. Human amniotic fluid for fertilization and culture of human embryos: results of clinical trials in human IVF programs. J In Vitro Fertil Embryo Transfer 1989;6:213–217.CrossRefGoogle Scholar
  33. 33.
    Bongso A, Ng SC, Fong CY, Ratnam SS. Co-cultures: a new lead in embryo quality improvement for assisted reproduction. Fertil Steril 1991;56: 179–191.PubMedGoogle Scholar
  34. 34.
    Menezo Y, Guerin J, Czyba J. Improvement of human embryo development in vitro by coculture on monolayers of Vero cells. Biol Reprod 1990;43:301–306.CrossRefGoogle Scholar
  35. 35.
    Rogers PAW, Milne B, Trounson AO. A model to show uterine receptivity and embryo viability following ovarian stimulation for in vitro fertilization. J In Vitro Fertil Embryo Transfer 1986;3:93–98.CrossRefGoogle Scholar
  36. 36.
    Leeton J, Shaw G, Short RV. Twin ovulations, dizygotic twins and human fecundability. Br J Obstet Gynaecol 1992.Google Scholar
  37. 37.
    Gras L, McBain J, Trounson A, Kola I. The incidence of chromosomal aneuploidies in stimulated and unstimulated (natural) uninseminated human oocytes. Hum Reprod 1992.Google Scholar
  38. 38.
    Lutjen PJ, Trounson A, Leeton J, Findlay J, Wood C, Renou P. The establishment and maintenance of pregnancy using in vitro fertilization and embryo donation with primary ovarian failure. Nature 1984;307:174–175.PubMedCrossRefGoogle Scholar
  39. 39.
    Trounson A. The development of the technique of oocyte donation and hormonal replacement therapy: is oestrogen really necessary for the establishment and maintenance of pregnancy. Reprod Fertil Dev 1992;4. Google Scholar
  40. 40.
    Navot D, Laufer N, Kopolovic J, et al. Artificially induced endometrial cyclesand establishment of pregnancies in the absence of ovaries. N Engl J Med 1986;314:806–818. PubMedCrossRefGoogle Scholar
  41. 41.
    Rosenwaks Z. Donor eggs: their application in modern reproductive technologies. Fertil Steril 1987;47:895–909.PubMedGoogle Scholar
  42. 42.
    Formigli L, Formigli G, Scelsi R, Roccio C, Belotti G, Noe C. Transfer of preembryos donated and fertilized in vivo to recipients given a steroid replacement therapy. Hum Reprod 1988;3:741–746.PubMedGoogle Scholar
  43. 43.
    Sauer MV, Paulson RJ, Macaso TM, Francis MM, Lobo RA. Oocyte and preembryo donation to women with ovarian failure: an extended clinical trial. Fertil Steril 1991;55:39–43.PubMedGoogle Scholar
  44. 44.
    Navot D, Scott, RT, Droesch K, Veeck LL, Liu H-C, Rosenwaks Z. The window of embryo transfer and the efficiency of human conception in vitro. Fertil Steril 1991;55:114–118.PubMedGoogle Scholar
  45. 45.
    Serhal P, Craft I. Simplified treatment for oocyte donation. Lancet 1987;1:687–688.PubMedCrossRefGoogle Scholar
  46. 46.
    de Ziegler D, Frydman R. Different implantation rates after transfers of cryopreserved embryos originating from donated oocytes or from regular in vitro fertilization. Fertil Steril 1990;54:682–8.PubMedGoogle Scholar
  47. 47.
    Check JH. Uterine receptivity in subjects with ovarian failure. Letter to the editor. Fertil Steril 1991;55:1208–1209.Google Scholar
  48. 48.
    Sauer MV, Paulson RJ, Macaso TM, Francis MM, Lobo RA. Oocyte and preembryo donation to women with ovarian failure: an extended clinical trial. Fertil Steril 1991;55:39–43.PubMedGoogle Scholar
  49. 49.
    Sauer MV, Paulson RJ, Lobo RA. A preliminary report on oocyte donation extending reproductive potential to women over 40. N Engl J Med 1990; 323:1157–1160.PubMedCrossRefGoogle Scholar
  50. 50.
    Levran D, Ben-Shlomo I, Dor J, Ben-Rafael Z, Nebel L, Mashiach S. Aging of endometrium and oocytes: observations on conception and abortion rates in an egg donation model. Fertil Steril 1991;56:1091–1094.PubMedGoogle Scholar
  51. 51.
    Calderon I, Kovacs G, Cushnahan L, Osborne J. GIFT vs TEST for male infertility [Abstract]. Proc 7th World Congress in IVF and Assisted Procreations, Paris, 1991.Google Scholar
  52. 52.
    Calderon I, Fuscaldo G, Yates C, Azuma K, Osborne J, Wood C. GIFT versus TEST for male infertility, a prospective randomized trial [Abstract]. Proc Am Fertil Soc 48th annu meet, New Orleans, Oct 1992.Google Scholar
  53. 53.
    Sobieszczuk D, Fuscaldo G, Poulos C, McLachlan R, Trounson A. Microdrop insemination vs microinjection/insemination in severe male factor patients [Abstract]. Proc Am Fertil Soc 48th annu meet, New Orleans, Oct 1992.Google Scholar
  54. 54.
    Sakkas D, Lacham O, Gianaroli L, Trounson A. Subzonal sperm microinjection in cases of severe male factor infertility and repeated in vitro fertilization failure. Fertil Steril 1992;57:1279–1288.PubMedGoogle Scholar
  55. 55.
    Malter HE, Cohen J. Partial zona dissection of the human oocyte:a nontraumatic method using micromanipulation to assist zona pellucida penetration. Fertil Steril 1989;51:139–148.PubMedGoogle Scholar
  56. 56.
    Cohen J, Malter H, Wright G, Kort H, Massey J, Mitchell D. Partial zona dissection of human oocytes when failure of zona pellucida penetration is anticipated. Hum Reprod 1989;4:435–442.PubMedGoogle Scholar
  57. 57.
    Garrisi GJ, Sapira V, Talansky BE, Navot D, Grunfeld L, Gordon JW. Clinical evaluation of three approaches to micromanipulation-assisted fertilization. Fertil Steril 1990;54:671–677.PubMedGoogle Scholar
  58. 58.
    Laws-King A, Trounson A, Sathananthan AH, Kola I. Fertilization of human oocytes by microinjection of a single spermatozoon under the zona pellucida. Fertil Steril 1987;48:637–642.PubMedGoogle Scholar
  59. 59.
    Ng SC, Bongso A, Sathananthan AH, et al. Pregnancy after transfer of multiple sperm under the zona. Lancet 1988;2:790.PubMedCrossRefGoogle Scholar
  60. 60.
    Fishel S, Johnson J, Jackson R, Gross S, Antimori S, Versaci C. Subzonal insemination for the alleviation of infertility. Fertil Steril 1990;54:828–835.PubMedGoogle Scholar
  61. 61.
    Trounson A, Sathananthan AH. Fertilization using micromanipulation techniques. In: Trounson AO, Gardner DG, eds. Handbook of in vitro fertilization. CRC Press, 1992. Google Scholar
  62. 62.
    Cohen J, Talansky BE, Malter HM, et al. Microsurgical fertilization and teratozoospermia. Hum Reprod 1991;6:118–123.PubMedGoogle Scholar
  63. 63.
    Lazendorf SE, Slussor MS, Maloney MK, Hodgen GD, Veek LL, Rosenwaks Z. A pre-clinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril 1988;49:835–842.Google Scholar
  64. 64.
    Martin RH, Ko E, Rademaker A. Human sperm chromosome complements after microinjection of hamster eggs. J Reprod Fertil 1988;84:179–186.PubMedCrossRefGoogle Scholar
  65. 65.
    Van Steirtegham A. Unpublished data.Google Scholar
  66. 66.
    Trounson A. Preservation of human eggs and embryos. Fertil Steril 1986;46:1–12.PubMedGoogle Scholar
  67. 67.
    Trounson AO. Cryopreservation. Br Med Bull 1990;46:695–708.PubMedGoogle Scholar
  68. 68.
    Trounson AO, Shaw JM. Embryo cryopreservation. Reprod Med Rev 1992.Google Scholar
  69. 69.
    Mazur P. Equilibrium, quasi-equilibrium, and non-equilibrium freezing of mammalian embryos. Cell Biophys 1990;17:53–92.PubMedGoogle Scholar
  70. 70.
    Rail WF. Factors affecting the survival of mouse embryos cryopreserved by vitrification. Cryobiology 1987;24:387–402.CrossRefGoogle Scholar
  71. 71.
    Trounson A, Peura A, Kirby C. Ultrarapid freezing: a new low-cost and effective method of cryopreservation. Fertil Steril 1987;48:822–826.Google Scholar
  72. 72.
    Shaw JM, Trounson AO. Effect of dimethyl sulfoxide and protein concentration on the viability of two-cell mouse embryos frozen with a rapid freezing technique. Cryobiology 1989;26:413–421.PubMedCrossRefGoogle Scholar
  73. 73.
    Shaw JM, Diotallevi L, Trounson AO. A simple rapid 4.5 M dimethylsulfoxide freezing technique for the cryopreservation of one-cell to blastocyst stage preimplantation mouse embryos. Reprod Fertil Dev 1991;3:621–626.PubMedCrossRefGoogle Scholar
  74. 74.
    Trounson AO, Sjoblom P. Cleavage and development of human embryos in vitro after ultrarapid freezing and thawing. Fertil Steril 1988;50:373–376.PubMedGoogle Scholar
  75. 75.
    Gordts S, Roziers P, Campo R, Noto V. Survival and pregnancy outcome after ultrarapid freezing of human embryos. Fertil Steril 1990;53:469–472.PubMedGoogle Scholar
  76. 76.
    Barg PE, Barad DH, Feichtinger W. Ultrarapid freezing (URF) of mouse and human preembryos:a modified approach. J In Vitro Fertil Embryo Transfer 1990;7:355–357.CrossRefGoogle Scholar
  77. 77.
    Whittingham DG. Fertilization in vitro and development to term of unfertilized mouse oocytes previously stored at -196°C. J Reprod Fertil 1977;49:89–94.PubMedCrossRefGoogle Scholar
  78. 78.
    Glenister PH, Wood MJ, Kirby C, Whittingham DG. The incidence of chromosome anomalies in first-cleavage mouse embryos obtained from frozen-thawed oocytes fertilized in vitro. Gamete Res 1987;16:205–216.PubMedCrossRefGoogle Scholar
  79. 79.
    Trounson A, Kirby C. Problems in the cryopreservation of unfertilized eggs by slow cooling in dimethyl sulfoxide. Fertil Steril 1989;52:778–786.PubMedGoogle Scholar
  80. 80.
    Schroeder AC, Champlin AK, Mobrnaten LE, Eppig JJ. Developmental capacity of mouse oocytes cryopreserved before and after maturation in vitro. J Reprod Fertil 1990;89:43–50.PubMedCrossRefGoogle Scholar
  81. 81.
    Trounson A, Shaw J. The cryopreservation of human eggs and embryos. In: Wallach E, ed. Reproductive medicine and surgery, 1992.Google Scholar
  82. 82.
    Tarin JJ, Handyside AH. Embryo biopsy strategies for preimplantation diagnosis. Fertil Steril 1992.Google Scholar
  83. 83.
    Verlinsky Y, Rechitoky S, Evsikov S, et al. Reconception and preimplantation diagnosis for cystic fibrosis. Prenat Diagn 1992;12:103–110.PubMedCrossRefGoogle Scholar
  84. 84.
    Navidi W, Arnheim N. Using PCR in preimplantation genetic disease diagnosis. Hum Reprod 1991;6:836–849.PubMedGoogle Scholar
  85. 85.
    Handyside AH, Pattinson JK, Penketh RJA, Delhanty JD, Winston RML, Tuddenham EDG. Biopsy of human preimplantation embryos and sexing by DNA amplification. Lancet 1989:347–349. Google Scholar
  86. 86.
    Handyside AH, Konogianni EH, Hardy K, Winston RML. Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature 1990;344:768–770.PubMedCrossRefGoogle Scholar
  87. 87.
    Handyside AH, Delhanty DA. Cleavage stage biopsy of human embryos and diagnosis of X-linked recessive disease. In: Edwards RG, ed. Preimplantation diagnosis of human genetic disease. Cambridge, UK: Cambridge University Press, 1992.Google Scholar
  88. 88.
    Grifo JA, Boyle A, Fischer E, et al. Preembryo biopsy and analysis of blastomeres by in situ hybridization. Am J Obstet Gynecol 1990;163:2013–2019.PubMedGoogle Scholar
  89. 89.
    Griffin DK, Handyside AH, Penketh RJA, Winston RML, Delhanty JDA. Fluorescent in situ hybridisation to interphase nuclei of human preimplantation embryos with X and Y chromosome specific probes. Hum Reprod 1991;6:101–105.PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1993

Authors and Affiliations

  • Alan Trounson

There are no affiliations available

Personalised recommendations