Abstract
Dissect young ears (2–4 cm long) from husk leaves and fix for 24 hours in Chemberlen’s fixative: 50% ethanol:40% formalin:glacial acetic acid in proportion 90:5:5, and rinse several times in 95% ethanol. Store in 70% ethanol in the refrigerator.
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References
Avalkina NA, Golubovskaya I, Peremislova E (1992) Mei-genes and female meiosis in maize. Genetics (Russian) 28(8): 130–141
Jongedijk E (1987) A quick enzyme squash technique for detailed studies of female meiosis in Solanum. Stain Technol 62: 135–142
Orlova IN, Avalkina NA (1985) An isolation of embryo-sacs of cereals without digestion of ovary tissue. Methodical Handbook, L. 12 pp (Russian)
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© 1994 Springer-Verlag New York, Inc.
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Golubovskaya, I., Avalkina, N.A. (1994). Protocol for Preparing Maize Macrospore Mother Cells for the Study of Female Meiosis and Embryo-sac Development. In: Freeling, M., Walbot, V. (eds) The Maize Handbook. Springer Lab Manuals. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2694-9_69
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DOI: https://doi.org/10.1007/978-1-4612-2694-9_69
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