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Gene Tagging with Ac/Ds Elements in Maize

  • Stephen L. Dellaporta
  • Maria A. Moreno
Part of the Springer Lab Manuals book series (SLM)

Abstract

A ctivator (Ac) and Ds (Dissociation) comprise a family of maize transposable elements. Ac is the autonomous member of the family, capable of producing a transposase factor needed for mobility. Ds elements are nonautonomous and capable of transposition only when transactivated by Ac (reviewed by Fedoroff 1989). The mobility of these elements provides a useful molecular genetic tool for gene tagging in maize.

Keywords

AciDs Element Transposition Event Maize Transposable Element Imprecise Excision Independent Transposition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Chen J, Greenblatt IM, Dellaporta SL (1987) Transposition of Ac from the P locus of maize into unreplicated chromosomal sites. Genetics 117: 109–116PubMedGoogle Scholar
  2. Chen J, Greenblatt IM, Dellaporta SL (1991) Molecular analysis of Ac transposition and DNA replication. Genetics 130: 665–676Google Scholar
  3. Chomet PS, Wessler SR, Dellaporta SL (1987) Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J 6: 295–302PubMedGoogle Scholar
  4. Dawe RK, Freeling M (1990) Clonal analysis of the cell lineages in the male flower of maize. Dev Biol 142: 233–245PubMedCrossRefGoogle Scholar
  5. Dellaporta SL, Greenblatt IM, Kermicle JL, Hicks JB, Wessler SR (1988) Molecular cloning of the R-nj gene by transposon tagging with Ac. In Gustafson JP, Appels R (eds) Chromosome Structure and Function: Impact of New Concepts, Plenum Press, New York, pp 263–282CrossRefGoogle Scholar
  6. Dellaporta SL, Moreno MA, DeLong A (1991) Cell lineage analysis of the maize gynoecium using Ac. Development s1: 141–147Google Scholar
  7. Fedoroff NV (1989). Maize transposable elements. In Berg DE, Howe MM (eds) Mobile DNA, Am Soc Microbiol, Washington DC, pp 377–411Google Scholar
  8. Greenblatt IM (1984) A chromosome replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element Modulator in maize. Genetics 108: 471–485PubMedGoogle Scholar
  9. Greenblatt IM, Brink RA (1962) Twin mutations in medium variegated pericarp in maize. Genetics 47: 489–501PubMedGoogle Scholar
  10. Kermicle JL (1984) Recombination between components of a mutable gene system in maize. Genetics 107: 489–500PubMedGoogle Scholar
  11. Moreno MA, Chen J, Greenblatt I, Dellaporta SL (1992) Reconstitutional mutagenesis of the maize P gene by short-range Ac transpositions. Genetics 131: 939–956PubMedGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • Stephen L. Dellaporta
  • Maria A. Moreno

There are no affiliations available

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