How RFLP Loci Can Be Used to Assist Transposon-Tagging Efforts
The purpose of this chapter is to enable the investigator to obtain and utilize restriction fragment-length polymorphism (RFLP) probes for the ultimate goal of cloning genes by transposon tagging. Probes for transposable elements often yield a complex RFLP pattern in maize DNA, and this chapter presents methods for interpreting such patterns. While purely genetic methods can be used to establish an association between an autonomous transposable element and a mutant locus (McClintock 1965), molecular methods such as those presented here are often required to perform a similar analysis with nonautonomous elements. The latter elements cause far more mutations in maize than do their autonomous counterparts.
KeywordsTransposable Element Target Locus Mutant Allele Mutant Locus RFLP Probe
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