Advertisement

How RFLP Loci Can Be Used to Assist Transposon-Tagging Efforts

  • Steven P. Briggs
  • William D. Beavis
Part of the Springer Lab Manuals book series (SLM)

Abstract

The purpose of this chapter is to enable the investigator to obtain and utilize restriction fragment-length polymorphism (RFLP) probes for the ultimate goal of cloning genes by transposon tagging. Probes for transposable elements often yield a complex RFLP pattern in maize DNA, and this chapter presents methods for interpreting such patterns. While purely genetic methods can be used to establish an association between an autonomous transposable element and a mutant locus (McClintock 1965), molecular methods such as those presented here are often required to perform a similar analysis with nonautonomous elements. The latter elements cause far more mutations in maize than do their autonomous counterparts.

Keywords

Transposable Element Target Locus Mutant Allele Mutant Locus RFLP Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burr B personal communication: Matz EC, Burr FA, Burr B (1991) Mapping new mutations using RFLPs. Maize Genetics Cooperation News Letter 65: 104–105Google Scholar
  2. Elston RC (1975) An approximation for the prior probability of autosomal linkage. Cytogen Cell Genet 14: 290–292CrossRefGoogle Scholar
  3. McClintock B (1965) The control of gene action in maize, In Genetic Control of Differentiation, Brookhaven Symposia in Biology 18: 162–184Google Scholar
  4. Martienssen R, Barkan A, Freeling M, Taylor W (1990) Somatically heritable switches in the DNA modification of Mu transposable elements monitored with a suppressible mutant in maize. Genes Dev 4: 331–343PubMedCrossRefGoogle Scholar
  5. Ott J (1985) Analysis of Human Genetic Linkage. John Hopkins, Baltimore, MD, 302 ppGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • Steven P. Briggs
  • William D. Beavis

There are no affiliations available

Personalised recommendations