Abstract
It has been known since the 1920s that vitamin A is required for normal spermatogenesis (1, 2). Depletion of vitamin A from the diet was shown to cause spermatogenic arrest and testicular atrophy, whereas the replenishment of retinol, the principal form of vitamin A, to the vitamin A-deficient (VAD) animals restored spermatogenesis (3). Since then, numerous investigators have analyzed the cell types that are present in the VAD testis (4–8) and described various spermatogenic depletion and retinol replenishment processes resulting in synchronization of spermatogenic stages in the replenished testes (9–15). However, to date, the molecular mechanism by which vitamin A is obligatory for spermatogenesis has not been elucidated. In fact, which cell types in the testis respond directly to the vitamin A signal is not completely clear. The challenge is to define which individual cells in the testis are the direct target of vitamin A and to understand how these cells interact with other cells to determine whether they proliferate, differentiate, survive, or degenerate. Recently, it has been established that nuclear retinoid receptors, members of the steroid/thyroid superfamily of receptors, which are now accepted as the transcription factors that mediate the retinoic acid signal in most tissues (16), are also important mediators of the vitamin A signals in testis (17–22).
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References
Wolbach SB, Howe PR. Tissue changes following deprivation of fat-soluble vitamin A. J Exp Med 1925;42:753–77.
Mason KE. Differences in testes injury and repair after vitamin A deficiency, vitamin E deficiency and inanition. Am J Anat 1933;52:153–239.
Thompson JN, Howell JMcC, Pitt GAJ. Vitamin A and reproduction in rats. Proc R Soc 1964;159:510–35.
Mitranond V, Sobhon P, Tosukhowong P, Chindaduangrat W. Cytological changes in the testes of vitamin A deficient rats. I. Quantitation of germinal cells in the seminiferous tubules. Acta Anat 1979;103:159–68.
Huang HFS, Hembree WC. Spermatogenic response to vitamin A in vitamin A deficient rats. Biol Reprod 1979;21:891–904.
Unni E, Rao MR, Ganguly J. Histological and ultrastructural studies on the effect of vitamin A depletion and subsequent repletion with vitamin A on germ cells and Sertoli cells in rat testis. Indian J Exp Biol 1983;21:180–92.
Griswold MD, Bishop PD, Kim KH, Ren P, Siiteri KE, Morales C. Function of vitamin A in normal and synchronized seminiferous tubules. Ann NY Acad Sci 1989;564:154–72.
Ismail N, Morales C, Clermont Y. Role of spermatogonia in the stage-synchronization of the seminiferous epithelium in vitamin A-deficient rats. Am J Anat 1990;188:57–63.
Morales CR, Griswold MD. Retinol-induced stage synchronization in seminiferous tubules of the rat. Endocrinology 1987;121:432–4.
Siiteri JE, Karl AF, Linder CC, Griswold MD. Testicular synchrony: evaluation and analysis of different protocols. Biol Reprod 1991;46:284–9.
Bartlett JM, Weinbauer GF, Nieschlag E. Stability of spermatogenic synchronization achieved by depletion and restoration of vitamin A in rats. Biol Reprod 1990;42:603–12.
Huang HFS, Marshall GR, Nieschlag E. Enrichment of the stages of the seminiferous epithelium in vitamin A-replaced vitamin A-deficient rats. J Reprod Fertil 1990;85:51–60.
van Beek MEAB, Meistrich ML. A method for quantifying synchrony in testes of rats treated with vitamin A deprivation and readministration. Biol Reprod 1990;42:424–31.
van Beek MEAB, Meistrich ML. Stage-synchronized seminiferous epithelium in rats after manipulation of retinol levels. Biol Reprod 1991;45:235–44.
van Pelt AMM, de Rooij DG. Synchronization of the seminiferous epithelium after vitamin A replacement in vitamin A-deficient mice. Biol Reprod 1990;43:363–7.
Giguère V. Retinoic acid receptors and cellular retinoid binding proteins: complex interplay in retinoid signaling. Endocr Rev 1994;15:61–79.
Kim KH, Wang ZQ. Action of vitamin A on testis: role of the Sertoli cell. In: Griswold MD, Russell L, eds. The Sertoli cell. Clearwater, FL: Cache River Press, 1993:514–35.
Kim KH, Griswold MD. The regulation of retinoic acid receptor mRNA levels during spermatogenesis. Mol Endocrinol 1990;4:1679–88.
Eskild W, Ree AH, Levy FO, Jahnsen T, Hansson V. Cellular localization of mRNAs for retinoic acid receptor a, cellular retinol-binding protein, and cellular retinoic acid-binding protein in rat testis: evidence for germ cell-specific mRNA. Biol Reprod 1991;44:53–61.
van Pelt AMM, van den Brink CE, de Rooij DG, van der Saag PT. Changes in retinoic acid receptor messenger ribonucleic acid levels in the vitamin A-deficient rat testis after administration of retinoids. Endocrinology 1992;131:344–50.
Wan YJ, Wang L, Wu TC. Detection of retinoic acid receptor mRNA in rat tissues by reverse transcriptase-polymerase chain reaction. J Mol Endocrinol 1992;9:291–4.
Huang HFS, Li MT, Pogach LM, Qian L. Messenger ribonucleic acid of rat testicular retinoic acid receptors: developmental pattern, cellular distribution, and testosterone effect. Biol Reprod 1994;51:541–50.
Setchell BP, Waites GMH. The blood-testis barrier. In: Hamilton DW, Greep RP, eds. Handbook of physiology, sect. 7: Endocrinology, vol. 5. Male reproductive system. Washington, DC: American Physiological Society, 1975: 143–72.
Ismail N, Morales CR. Effects of vitamin A deficiency on the inter-Sertoli junctions and on the germ cell population. Microsc Res Tech 1992;20(l):43–9.
Leblond CP, Clermont Y. Definition of the stages of the cycle of the seminiferous epithelium in the rat. Ann NY Acad Sci 1952;55:548–73.
van Pelt AMM, de Rooij DG. The origin of the synchronization of the seminiferous epithelium in vitamin A-deficient rats after vitamin A replacement. Biol Reprod 1990;42:677–82.
Strausfeld U, Labbé JC, Fesquet D, Cavadore JC, Picard A, Sadhu K, Russell P, Doree M. Dephosphorylation and activation of a p34cdc2/cyclin B complex in vitro by human CDC25 protein. Nature 1991;351:242–5.
Wang ZQ, Kim KH. Vitamin A-deficient testis germ cells are arrested at the end of S phase of the cell cycle: a molecular study of the origin of synchronous spermatogenesis in regenerated seminiferous tubules. Biol Reprod 1993; 48:1157–65.
de Rooij DG, van Dissel-Emiliani FMF, van Pelt AMM. Regulation of spermatogonial proliferation. Ann NY Acad Sci 1989;564:140–53.
Hilscher W. DNA synthesis: proliferation and regeneration of the spermatogonia in the rat. Arch Anat Microsc Morphol Exp 1967; 56:75–84.
Huckins C. The spermatogonial stem cell population in adult rats. I. Their morphology, proliferation and maturation. Anat Res 1971;169:533–58.
Howell JMcC, Thompson JN, Pitt GAJ. Histology of the lesions produced in the reproductive tract of animals fed a diet deficient in vitamin A alcohol but containing vitamin A acid. I. The male rat. J Reprod Fertil 1963;5:159–67.
Blaner WS, Olson JA. Retinol and retinoid acid metabolism. In: Sporn MB, Roberts AB, Goodman DS, eds. The retinoids, 2nd ed. New York: Raven Press, 1994:244–5.
van Beek MEAB, Meistrich ML. Spermatogenesis in retinol-deficient rats maintained on retinoic acid. J Reprod Fertil 1992;94:327–36.
Haneji T, Koide SS, Nishimune Y, Oota Y. Dibutyryl adenosine cyclic monophosphate regulates differentiation of type A spermatogonia with vitamin A in adult mouse cryptorchid testis in vitro. Endocrinology 1986;119:2490–6.
Bishop PD, Griswold MD. Uptake and metabolism of retinol in cultured Sertoli cells: evidence for a kinetic model. Biochemistry 1987;26:7511–8.
Shingleton JL, Skinner MK, Ong DE. Retinol esterification in Sertoli cells by lecithin-retinol acyltransferase. Biochemistry 1989;28:9647–53.
van Pelt AMM, de Rooij DG. Retinoic acid is able to reinitiate spermatogenesis in vitamin A-deficient rats and high replicate doses support the full development of spermatogenic cells. Endocrinology 1991;128:697–704.
Davis JT, Ong DE. Retinol processing by the peritubular cell from rat testis. Biol Reprod 1995;52:356–64.
Porter SB, Ong DE, Chytil F, Orgebin-Crist M-C. Localization of cellular retinol-binding protein and cellular retinoic acid binding-protein in the rat testis and epididymis. J Androl 1985;6:197–212.
Ong DE, Newcomer ME, Chytil F. Cellular retinoid-binding proteins. In: Sporn MB, Roberts AB, Goodman DS, eds. The retinoids, 2nd ed. New York: Raven Press, 1994: 283–318.
Rajan N, Blaner WS, Soprano DR, Suhara A, Goodman DS. Cellular retinol-binding protein messenger RNA levels in normal and retinoid-deficient rats. J Lipid Res 1990;31:821–9.
Eskild W, Oyen O, Beebe S, Jahnsen T, Hasson V. Regulation of mRNA levels for cellular retinol binding protein in rat Sertoli cells by cyclic AMP and retinol. Biochem Biophy Res Commun 1988;152:1504–10.
Husmann M, Hoffmann B, Stump DG, Chytil F, Pfahl M. A retinoic acid responsive element from the rat CRBPI promoter is activated by an RAR/RXR heterodimer. Biochem Biophys Res Commun 1992;187:1558–64.
Heyman RA, Mangelsdorf DJ, Dyck JA, Stein RB, Eichele G, Evans RM, Thaller C. 9-cis retinoic acid is a high affinity ligand for retinoid X receptor. Cell 1992;68:397–406.
Lufkin T, Lohnes D, Mark M, Dierich A, Gorry P, Gaub M-P, et al. High postnatal lethality and testis degeneration in retinoic acid receptor α mutant mice. Proc Natl Acad Sci USA 1993;90:7225–9.
Mendelsohn C, Mark M, Dolle P, Dierich A, Gaub MP, Krust A, et al. Retinoic acid receptor β2 (RARβ2) null mutant mice appear normal. Dev Biol 1994; 166:246–58.
Lohnes D, Kastner P, Dierich A, Mark M, Lemeur M, Chambon P. Function of retinoic acid receptor γ in the mouse. Cell 1993;73:643–58.
Wang ZQ, Kim KH. Retinol differentially regulates male germ cell-associated kinase (mak) messenger ribonucleic acid expression during spermatogenesis. Biol Reprod 1993;49:951–64.
Zhengwei Y, Wreford NG, de Kretser DM. A quantitative study of spermatogenesis in the developing rat testis. Biol Reprod 1990;43:629–35.
Orth J. Proliferation of Sertoli cells in fetal and postnatal rats: a quantitative autoradiographic study. Anat Rec 1982;203:485–92.
Collard MW, Griswold MD. Biosynthesis and molecular cloning of sulfated glycoprotein 2 secreted by rat Sertoli cells. Biochemistry 1987;26:3297–303.
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Kim, K.H., Akmal, K.M. (1996). Role of Vitamin A in Male Germ-Cell Development. In: Desjardins, C. (eds) Cellular and Molecular Regulation of Testicular Cells. Serono Symposia USA Norwell, Massachusetts. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2374-0_7
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DOI: https://doi.org/10.1007/978-1-4612-2374-0_7
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