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Testis-Specific Gene Transcription

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Book cover Cellular and Molecular Regulation of Testicular Cells

Part of the book series: Serono Symposia USA Norwell, Massachusetts ((SERONOSYMP))

Abstract

The classic studies of Monesi (1,2) in the 1960s revealed an intricate pattern of RNA synthesis during spermatogenesis, characterized by transient transcriptional activity. During the early stages of meiotic prophase, a relatively low rate of [3H]uridine incorporation was detected; however, RNA synthesis increases at the mid-pachytene stage followed by diminution during the meiotic divisions. RNA synthesis once again increases in the round and elongating spermatids (up to step 8), followed by a decline in later stages where nuclear condensation occurs.

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References

  1. Monesi V. Ribonucleic acid synthesis during mitosis and meiosis in the mouse testis. J Cell Biol 1964;22:521–32.

    Article  PubMed  CAS  Google Scholar 

  2. Monesi V. Synthetic activities during spermatogenesis in the mouse: RNA and protein. Exp Cell Res 1965;39:197–224.

    CAS  Google Scholar 

  3. Hake LE, Hecht NB. Utilization of an alternative transcription initiation site of somatic cytochrome c in the mouse produces a testis-specific cytochrome c mRNA. J Biol Chem 1993;268:4788–97.

    PubMed  CAS  Google Scholar 

  4. Ito E, Toki T, Ishihara H, Ohtani H, Gu L, Yokoyama M, Engel JD, Yamamoto M. Erythroid transcription factor GATA-1 is abundantly transcribed in mouse testis. Nature 1993;362:466–8.

    Article  PubMed  CAS  Google Scholar 

  5. Gizang-Ginsberg E, Wolgemuth DJ. Expression of the pro-opiomelanocortin gene is developmentally regulated and affected by germ cells in the male mouse reproductive system. Proc Natl Acad Sci USA 1987;84:1600–4.

    Article  PubMed  CAS  Google Scholar 

  6. Kilpatrick DL, Millette CF. Expression of pro-enkephalin messenger RNA by mouse spermatogenic cells. Proc Natl Acad Sci USA 1986;83:5015–8.

    Article  PubMed  CAS  Google Scholar 

  7. Propst F, Rosenberg MP, Van de Woude GF. Proto-oncogene expression in germ cell development. Trends Genet 1988;4:183–7.

    Article  PubMed  CAS  Google Scholar 

  8. Yeom YI, Abe K, Bennett D, Artzt K. Testis/embryo-expressed genes are clustered in the mouse H-2K region. Proc Natl Acad Sci USA 1992;89:773–7.

    Article  PubMed  CAS  Google Scholar 

  9. Ha H, Howard CA, Yeom YI, Abe K, Uehara H, Artzt K, Bennett D. Several testis-expressed genes in the mouse t-complex have expression differences between wild-type and t-mutant mice. Dev Genet 1991;12:318–32.

    Article  PubMed  CAS  Google Scholar 

  10. Rappold GA, Stubbs L, Labeit S, Crkvenjakov RB, Lehrach H. Identification of a testis-specific gene from the mouse t-complex next to a CpG-rich island. EMBO J 1987;6:1975–80.

    PubMed  CAS  Google Scholar 

  11. Willison KR, Hynes G, Davies P, Goldsborough A, Lewis VA. Expression of three t-complex genes, Tcp-1, D17Lehll7c3, and D17Leh66, in purified murine spermatogenic cell populations. Genet Res 1990;56:193–201.

    Article  PubMed  CAS  Google Scholar 

  12. Silver L. Mouse r-haplotypes. Annu Rev Genet 1985;19:179–208.

    Article  PubMed  CAS  Google Scholar 

  13. Lyon MR Transmission ratio distortion in mouse t-haplotype is due to multiple distorter genes acting on a responder locus. Cell 1984;37:621–8.

    Article  PubMed  CAS  Google Scholar 

  14. Lyon MR Male sterility of the mouse t-complex is due to homozygosity of the distorter genes. Cell 1986;44:357–63.

    Article  PubMed  CAS  Google Scholar 

  15. McCarrey JR, Thomas K. Human testis-specific PGK gene lacks introns and possesses characteristics of a processed gene. Nature 1987;326:501–5.

    Article  PubMed  CAS  Google Scholar 

  16. McCarrey JR. Nucleotide sequence of the promoter region of a tissue-specific human retroposon: comparison with its housekeeping progenitor. Gene 1987;61:291–8.

    Article  PubMed  CAS  Google Scholar 

  17. Ashworth A, Skene B, Swift S, Lovell-Badge R. Zfa is an expressed retro-transposon derived from an alternative transcript of the Zfx gene. EMBO J 1990;9:1529–34.

    PubMed  CAS  Google Scholar 

  18. Fitzgerald J, Hutchison WM, Dahl HH. Isolation and characterization of the mouse pyruvate dehydrogenase E1 alpha genes. Biochim Biophys Acta 1991;1131:83–90.

    Google Scholar 

  19. Soares MB, Schon E, Henderson A, Karathanasis SK, Cate R, Zeitlin S, Chirwin J, Efstratiadis A. RNA-mediated gene duplication: the rat pre-proinsulin I gene is a functional retroposon. Mol Cell Biol 1985;5:2090–103.

    PubMed  CAS  Google Scholar 

  20. Fourel G, Transy C, Tennant BC, Buendia MA. Expression of the woodchuck N-myc2 retroposon in brain and in liver tumors is driven by a cryptic N-myc promoter. Mol Cell Biol 1992;12:5336–44.

    PubMed  CAS  Google Scholar 

  21. Kuhn R, Kuhn C, Borsch D, Glatzer KH, Schafer U, Schafer M. A cluster of four genes selectively expressed in the male germ line of Drosophila melanogaster. Mech Dev 1991;35:143–51.

    Article  PubMed  CAS  Google Scholar 

  22. Richter JD, Paris J, McGrew LL. Maternal mRNA expression in early development: regulation at the 3′ end. Enzyme 1990;44:129–46.

    PubMed  CAS  Google Scholar 

  23. Richter JD. Translational control during early development. Bioessays 1991; 13:179–83.

    Article  PubMed  CAS  Google Scholar 

  24. Schafer M, Kuhn R, Bosse F, Schafer U. A conserved element in the leader mediates post-meiotic translation as well as cytoplasmic polyadenylation of Drosophila spermatocyte mRNA. EMBO J 1990;9:4519–25.

    PubMed  CAS  Google Scholar 

  25. Kempe E, Muhs B, Schafer M. Gene regulation in Drosophila spermatogenesis: analysis of protein binding at the translational control element TCE. Dev Genet 1992;14:449–59.

    Article  Google Scholar 

  26. Heidaran MA, Showman RM, Kistler WS. A cytochemical study of the transcriptional and translational regulation of nuclear transition protein 1 (TP1), a major chromosomal protein of mammalian spermatids. J Cell Sci 1988; 106:1427–33.

    CAS  Google Scholar 

  27. Morales CR, Kwon YK, Hecht NB. Cytoplasmic localization during storage and translation of the mRNAs of transition protein 1 and protamine 1, two translationally regulated transcripts of the mammalian testis. J Cell Sci 1991;100:119–31.

    PubMed  CAS  Google Scholar 

  28. Gold B, Fujimoto H, Kramer JM, Erickson RP, Hecht NB. Haploid accumulation and translational control of phosphoglycerate kinase-2 messenger RNA during mouse spermatogenesis. Dev Biol 1983;98:392–9.

    Article  PubMed  CAS  Google Scholar 

  29. Kleene KC, Distel RJ, Hecht NB. Translational regulation and deadenylation of a protamine mRNA during spermatogenesis in mouse. Dev Biol 1984;105:71–9.

    Article  PubMed  CAS  Google Scholar 

  30. Braun RE, Peschon JJ, Behringer RR, Brinster RL, Palmiter RD. Protamine 3′-untranslated sequences regulate temporal translational control and subcellular localization of growth hormone in spermatids of transgenic mice. Genes Dev 1989;3:793–802.

    Article  PubMed  CAS  Google Scholar 

  31. Kwon YK, Hecht NB. Cytoplasmic protein binding to highly conserved sequences in the 3′ untranslated region of mouse protamine 2 mRNA, a translationally regulated transcript of male germ cells. Proc Natl Acad Sci USA 1991;88:3584–8.

    Article  PubMed  CAS  Google Scholar 

  32. Kwon YK, Hecht NB. Binding of a phosphoprotein to the 3′ untranslated region of the mouse protamine 2 mRNA temporally represses its translation. Mol Cell Biol 1993;13:6547–57.

    PubMed  CAS  Google Scholar 

  33. Kwon YK, Murray MT, Hecht NB. Proteins homologous to the Xenopus germ cell-specific RNA-binding proteins p54/p56 are temporally expressed in mouse male germ cells. Dev Biol 1993;158:90–100.

    Article  CAS  Google Scholar 

  34. Hecht NB. Gene expression during male germ cell development. In: Desjardins C, Ewing LL, eds. Cell and molecular biology of the testis. Oxford, England: Oxford University Press, 1993:400–32.

    Google Scholar 

  35. Blanco A, Zinkham WH. Lactate dehydrogenases in human testes. Science 1963;139:601–2.

    Article  PubMed  CAS  Google Scholar 

  36. Goldberg E. Lactate and malic dehydrogenases in human spermatozoa. Science 1963;139:602–3.

    Article  PubMed  CAS  Google Scholar 

  37. Alcivar AA, Trasler JM, Hake LE, Salehi-Ashtiani K, Goldberg E, Hecht NB. DNA methylation and expression of the genes coding for lactate dehydrogenase A and C during rodent spermatogenesis. Biol Reprod 1991;44:527–35.

    Article  PubMed  CAS  Google Scholar 

  38. Vandeberg JL, Cooper DW, Close PJ. Testis specific phosphoglycerate kinase B in mouse. J Exp Zool 1976;198:231–40.

    Article  PubMed  CAS  Google Scholar 

  39. Vandeberg JL, Lee CY, Goldberg E. Immunohistochemical localization of phosphoglycerate kinase isozymes in mouse testis. J Exp Zool 1981;217:435–41.

    Article  PubMed  CAS  Google Scholar 

  40. Bluthmann H, Cicurel L, Kuntz GW, Haedenkamp G, Illmensee K. Immunohistochemical localization of mouse testis-specific phosphoglycerate kinase (PGK-2) by monoclonal antibodies. EMBO J 1982;1:479–84.

    PubMed  CAS  Google Scholar 

  41. Tilghman SM. DNA methylation: a phoenix rises. Proc Natl Acad Sci USA 1993;90:8761–2.

    Article  PubMed  CAS  Google Scholar 

  42. Goldberg E, Sberna D, Wheat TE, Urbanski GJ, Margoliash E. Cytochrome c: immunofluorescent localization of the testis specific form. Science 1997; 196:1010–2.

    Article  Google Scholar 

  43. Virbasius JV, Scarpulla RC. Structure of rodent genes encoding the testis-specific cytochrome c. J Biol Chem 1988;263:6791–6.

    PubMed  CAS  Google Scholar 

  44. Hess RA, Miller LA, Kirby JD, Margoliash E, Goldberg E. Immunoelectron microscope localization of testicular and somatic cytochrome c in the seminiferous epithelium of the rat. Biol Reprod 1993;48:1299–308.

    Article  PubMed  CAS  Google Scholar 

  45. Liang Z-G, O’Hern PA, Yavetz B, Yavetz H, Goldberg E. Human testis cDNA identified by sera from infertile patients: a molecular biology approach to immunocontraceptive development. Reprod Fertil 1994;6:297–305.

    Article  CAS  Google Scholar 

  46. Villasante A, Wang D, Dobner P, Dolph P, Lewis SA, Cowan NJ. Six mouse α-tubulin mRNAs encode five distinct isotypes: testis specific expression of two sister genes. Mol Cell Biol 1986;6:2409–19.

    PubMed  CAS  Google Scholar 

  47. Grimes SR, Wolfe SA, Anderson JV, Stein GS, Stein JL. Structural and functional analysis of the rat testis-specific histone H1t gene. J Cell Biochem 1990;44:1–17.

    Article  PubMed  CAS  Google Scholar 

  48. Moss SB, Challoner PB, Groudine M. Expression of a novel histone 2B during mouse spermatogenesis. Dev Biol 1989;133:83–92.

    Article  PubMed  CAS  Google Scholar 

  49. Kleene KC, Flynn JF. Characterization of a cDNA clone encoding a basic protein, TP2, involved in chromatin condensation during spermatogenesis in the mouse. J Biol Chem 1987;262:17272–7.

    PubMed  CAS  Google Scholar 

  50. Kleene KC, Bozorgzadeh A, Flynn JF, Yelick PC, Hecht NB. Nucleotide sequence of a cDNA encoding mouse transition protein 1. Biochim Biophys Acta 1988;950:215–20.

    PubMed  CAS  Google Scholar 

  51. Howard T, Balogh R, Overbeek P, Bernstein KE. Sperm-specific expression of angiotensin-converting enzyme (ACE) is mediated by a 91-base-pair promoter containing a CRE-like element. Mol Cell Biol 1993;13:18–27.

    PubMed  CAS  Google Scholar 

  52. Johnson PA, Peschon JJ, Yelick PC, Palmiter RD, Hecht NB. Sequence homologies in the mouse protamine 1 and 2 genes. Biochem Biophys Acta 1988;950:45–53.

    PubMed  CAS  Google Scholar 

  53. Kasahara M, Gutknecht J, Brew K, Spurr N, Goodfellow PN. Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene. Genomics 1989;5:527–34.

    Article  PubMed  CAS  Google Scholar 

  54. Mizuki N, Sarapata DE, Garcia-Sanz JA, Kasahara M. The mouse male germ cell-specific gene Tpx-1: molecular structure, mode of expression in spermatogenesis, and sequence similarity to two non-mammalian genes. Mammalian Genome 1992;3:274–80.

    Article  PubMed  CAS  Google Scholar 

  55. Mitchell MJ, Woods DR, Tucker PK, Opp JS, Bishop CE. Homology of a candidate spermatogenic gene from the mouse Y chromosome to the ubiquitin-activating enzyme E1. Nature 1991;354:483–6.

    Article  PubMed  CAS  Google Scholar 

  56. Boissonneault G, Lau YF. A testis-specific gene encoding a nuclear high-mobility-group box protein located in elongating spermatids. Mol Cell Biol 1993;13:4323–30.

    PubMed  CAS  Google Scholar 

  57. Ponzetto C, Wolgemuth DJ. Haploid expression of a unique c-abl transcript in the mouse male germ line. Mol Cell Biol 1985;5:1791–4.

    PubMed  CAS  Google Scholar 

  58. Goldman DS, Kisseling AA, Millette CF, Cooper GM. Expression of c-mos RNA in germ cells of male and female mice. Proc Natl Acad Sci USA 1987;84:4509–13.

    Article  PubMed  CAS  Google Scholar 

  59. Watrin F, Scotto L, Assoian RK, Wolgemuth DJ. Cell lineage specificity of expression of the murine transforming growth factor β3 and transforming growth factor β1 genes. Cell Growth Diff 1991;2:77–83.

    PubMed  CAS  Google Scholar 

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Authors
  1. Kourosh Salehi-Ashtiani
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  2. Erwin Goldberg
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Editor information

Editors and Affiliations

  1. Department of Physiology, School of Medicine, University of Virginia, Charlottesville, VA, 22908, USA

    Claude Desjardins Ph.D.

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© 1996 Springer-Verlag New York, Inc.

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Salehi-Ashtiani, K., Goldberg, E. (1996). Testis-Specific Gene Transcription. In: Desjardins, C. (eds) Cellular and Molecular Regulation of Testicular Cells. Serono Symposia USA Norwell, Massachusetts. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-2374-0_10

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  • DOI: https://doi.org/10.1007/978-1-4612-2374-0_10

  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4612-7519-0

  • Online ISBN: 978-1-4612-2374-0

  • eBook Packages: Springer Book Archive

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