New Perspectives on Ca2+ Influx in Mast Cells

  • Michael A. McCloskey


The importance of extracellular calcium for anaphylactic release of histamine was suggested by experiments of Mongar and Schild in 1958, years before the discovery of IgE or its high-affinity receptor, the FcεRI.1 Many subsequent studies using radiotracer flux and fluorescent Ca2+ indicators revealed that multivalent binding of antigen to the IgE-FcεRI complex elicits the release of internal as well as the influx of extracellular Ca2+, and there appears to be a causal relation between these two events. For the RBL-2H3 mast cell line used in many of these studies,2 it is generally agreed that Ca2+ influx is crucial for antigen-induced secretion of preformed inflammatory mediators.3


Mast Cell Electrophoretic Mobility Shift Assay Store Depletion Mast Cell Line CRAC Channel 
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  • Michael A. McCloskey

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