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Adeno—Associated Virus (AAV) Vectors for Musculoskeletal Gene Transfer

  • Xiao Xiao
  • Ryan Pruchnic
  • Juan Li
  • Johnny Huard
Part of the Methods in Bioengineering book series (MB)

Abstract

Effective gene delivery techniques into musculoskeletal tissues for gene therapy will revolutionize molecular medicine and pave the way for advances in orthopaedic treatment. However, numerous gene transfer approaches, including direct (in vivo), indirect (ex vivo), and systemic delivery of nonviral and viral vectors, have been hampered by limitations such as low transfection efficiency, immunologic responses, cytotoxic effects, and maturation-dependent factors that preclude the vector from transducing postmitotic and/or mature tissues. As a result, general application of gene therapy for treatments of muscular diseases and orthopedic injuries and repair will not be realized until improvement is made in vector development (see Chapter 14). Recent progress in the development adeno-associated virus (AAV), a small, nonpathogenic human DNA virus, has shed new light on this frontier.

Keywords

Gene Transfer Injured Muscle Site Specific Integration Mediate Gene Transfer Limb Girdle Muscular Dystrophy 
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© Birkhäuser Boston 2000

Authors and Affiliations

  • Xiao Xiao
  • Ryan Pruchnic
  • Juan Li
  • Johnny Huard

There are no affiliations available

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