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The Testis pp 143-150 | Cite as

Characterization of the Testicular Histone-Binding Protein, NASP

  • Michael G. O’Rand
  • Iglika N. Batova
  • Richard T. Richardson
Part of the Serono Symposia USA book series (SERONOSYMP)

Abstract

The nuclear autoantigenic sperm protein (NASP) was first recognized because of its autoantigenicity in males and immunological cross-reactivity with the sperm-specific autoantigen RSA (1). NASP was initially described as a highly immunogenic testis and sperm-specific protein, which was present in the postacrosomal region of mature spermatozoa and in the nucleus of developing spermatogenic cells (1,2). From DNA sequence comparisons, NASP appears to have evolved from the N1/N2 gene expressed in oocytes of Xenopus laevis (3,4). Indeed, the 3’ untranslated sequence identity between the rabbit and Xenopus mRNAs reaches approximately 60%, implying that mammalian NASP is a true homologue of N1/N2.

Keywords

Spermatogenic Cell Round Spermatid Primary Spermatocyte Xenopus Laevis Oocyte Chromatin Assembly Factor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Welch JE, Zimmerman LJ, Joseph DR, O’Rand MG. Characterization of a sperm-specific nuclear autoantigenic protein. I. Complete sequence and homology with the Xenopusprotein N1/N2. Biol Reprod 1990; 43: 559 – 68.PubMedCrossRefGoogle Scholar
  2. 2.
    Welch JE, O’Rand MG. Characterization of a sperm-specific nuclear autoantigenic protein. II. Expression and localization in the testis. Biol Reprod 1990;43:569–78.PubMedCrossRefGoogle Scholar
  3. 3.
    Krohne G . Immunological identification of the karyophilic, histone-binding proteins N1 and N2 in somatic cells and oocytes of diverse amphibia. Exp Cell Res 1985;158:205–22.PubMedCrossRefGoogle Scholar
  4. 4.
    Kleinschmidt JA, Dingwall C, Maier G, Franke WW. Molecular characterization of a karyophilic histone binding protein: cDNA cloning, amino acid sequence and expression of nuclear protein N1/N2 of Xenopus laevis. EMBO J 1986;5:3547–52.PubMedGoogle Scholar
  5. 5.
    Richardson RT, Batova I, O’Rand MG. Characterization of testicular and somatic NASP, mouse histone binding proteins. Mol Bio Cell 1998;9(Suppl.):178a.Google Scholar
  6. 6.
    Kleinschmidt JA, Fortkamp E, Krohne G, Zentgraf H, Franke WW. Co-existence of two different types of soluble histone complexes in nuclei of Xenopus laevis oocytes. J Biol Chem 1985;260:1166–76.PubMedGoogle Scholar
  7. 7.
    Kleinschmidt JA, Seiter A. Identification of domains involved in nuclear uptake and histone binding of protein N1 of Xenopus laevis. EMBO J 1988;7:1605–14.PubMedGoogle Scholar
  8. 8.
    Batova I, O’Rand MG. Histone binding domains in a human nuclear autoantigenic sperm protein. Biol Reprod 1996;54:1238–44.PubMedCrossRefGoogle Scholar
  9. 9.
    Smith AE, Kalderon D, Roberts BL, Colledge WH, Edge M, Gillett P, et al. The nuclear localization signal. Proc R Soc Lond 1985;226:43–58.PubMedCrossRefGoogle Scholar
  10. 10.
    Robbins J, Dilworth SM, Laskey RA, Dingwall C. Two interdependent basic domains in nueleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequence. Cell 1991;64: 615–23.PubMedCrossRefGoogle Scholar
  11. 11.
    Pabo CO, Sauer RT. Transcription factors: structural families and principles of DNA recognition. Ann Rev Biochem 1992;61:1053–95.PubMedCrossRefGoogle Scholar
  12. 12.
    Lapeyre B, Bourbon H, Amalric F. Nucleolin, the major nucleolar protein of grow¬ing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence. Proc Natl Acad Sci USA 1987;84:1472–76.PubMedCrossRefGoogle Scholar
  13. 13.
    O’Rand MG, Richardson RT, Zimmerman LJ Widgren EE. Sequence and localization of human NASP: conservation of a Xenopus histone binding protein. Dev Biol 1992;154:37–44.PubMedCrossRefGoogle Scholar
  14. 14.
    Gerace L, Blobel G. The nuclear envelope is reversibly depolymerized during mitosis. Cell 1980;19:277–87.PubMedCrossRefGoogle Scholar
  15. 15.
    Maul GG, French BT, Bechtol KB. Identification of lamins during nuclear differentiation in mouse spermatogenesis. Dev Biol 1986;115:68–77.PubMedCrossRefGoogle Scholar
  16. 16.
    Moss SB, Donovan MJ, Bellve AR. The occurrence and distribution of lamin proteins during mammalian spermatogenesis and early development. Ann NY Acad Sci 1987;513:74–89.PubMedCrossRefGoogle Scholar
  17. 17.
    Furukawa K, Hotta Y. cDNA cloning of a germ cell specific lamin B3 from mouse spermatocytes and analysis of its function by ectopic expression in somatic cells. EMBO J 1993;12:97–106.PubMedGoogle Scholar
  18. 18.
    Furukawa K, Inagaki H, Hotta Y. Identification and cloning of an mRNA coding for a germ cell-specific A-type lamin in mice. Exp Cell Res 1994;212:426–30.PubMedCrossRefGoogle Scholar
  19. 19.
    Meistrich ML . Histone and basic nuclear protein transitions in mammalian spermatogenesis. In: Hnilica LS, Stein GS, Stein JL, eds. Histones and other basic nuclear proteins. Boca Raton, FL: CRC Press, 1989:165–82.Google Scholar
  20. 20.
    Oko RJ, Jano V, Wagner CL, Kistler WS, Hermo LS. Chromatin reorganization in rat spermatids during the disappearance of testis-specific histone, Hit, and the appearance of transition proteins TP1 and TP2. Biol Reprod 1996;54:1141–57.PubMedCrossRefGoogle Scholar
  21. 21.
    Chen HY, Sun JM, Zhang Y, Davie JR, Meistrich ML. Ubiquitination of histone H3 in elongating spermatids of rat. J Biol Chem 1998;273:13165–69.PubMedCrossRefGoogle Scholar
  22. 22.
    Lee YH, O’Rand MG. Ultrastructural localization of a nuclear autoantigenic sperm protein in spermatogenic cells and spermatozoa. Anat Rec 1993;236:442–48.PubMedCrossRefGoogle Scholar
  23. 23.
    Batova I, Ingledue TC, Richardson RT, Marzluff WF, O’Rand MG. Nuclear translocation and histone binding of mouse testis NASP. Mol Bio Cell 1998;9 (Suppl.):178a.Google Scholar
  24. 24.
    Earnshaw WC . Anionic regions in nuclear proteins. J Cell Biol 1987;105:1479–82.PubMedCrossRefGoogle Scholar
  25. 25.
    Meistrich ML, Bucci LR, Trostle-Weige PK, Brock WA. Histone variants in rat spermatogonia and primary spermatocytes. Dev Biol 1985;112:230–40.PubMedCrossRefGoogle Scholar
  26. 26.
    Kim YJ, Hwang I, Tres LL, Kierszenbaum AL, Chae CB. Molecular cloning and differential expression of somatic and testis–specific H2B histone genes during rat spermatogenesis. Dev Biol 1987;124:23–34.PubMedCrossRefGoogle Scholar
  27. 27.
    Kremer EJ Kistler WS. Localization of mRNA for testis-specific histone Hlt by in situ hybridization. Exp Cell Res 1991;197:330–32.PubMedCrossRefGoogle Scholar
  28. 28.
    Moss SB, Orth JM. Localization of a spermatid-specific histone 2B protein in mouse spermiogenic cells. Biol Reprod 1993;48:1047–56.PubMedCrossRefGoogle Scholar
  29. 29.
    Unni E, Zang Y, Kangasniemi M, Saperstein W, Moss SB, Meistrich M. Stage-specific distribution of the spermatid-specific histone 2B in the rat testis. Biol Reprod 1995;53:820–26.PubMedCrossRefGoogle Scholar
  30. 30.
    Trostle-Weige PK, Meistrich ML, Brock WA, Nishioka K. Isolation and characterization of TH3, a germ cell-specific variant of histones in rat testis. J Biol Chem 1984;259:8769–76.PubMedGoogle Scholar
  31. 31.
    Dilworth SM, Black SJ, Laskey RA. Two complexes that contain histones are required for nucleosome assembly in vitro: role of nucleoplasmin and N1 in Xenopus egg extracts. Cell 1987;51:1009–18.PubMedCrossRefGoogle Scholar
  32. 32.
    Cobb J, Cargile B, Handel MA. Acquisition of competence to condense metaphase I chromosomes during spermatogenesis. Dev Biol 1999;205:49–64.PubMedCrossRefGoogle Scholar
  33. 33.
    Ito T, Bulger M, Kobayashi R, Kadonaga JT. Drosophila NAP-1 is a core histone chaperone that functions in ATP-facilitated assembly of regularly spaced nucleosomal arrays. Mol Cell Biol 1996;16:3112–24.PubMedGoogle Scholar
  34. 34.
    McQuibban GA, Commisso-Cappelli CN, Lewis PN. Assembly, remodeling, and histone binding capabilities of yeast nucleosome assembly protein 1. J Biol Chem 1998;273:6582–90.PubMedCrossRefGoogle Scholar
  35. 35.
    Kaufman PD, Kobayashi R, Kessler N, Stillman B. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication. Cell 1995;81:1105–14.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 2000

Authors and Affiliations

  • Michael G. O’Rand
  • Iglika N. Batova
  • Richard T. Richardson

There are no affiliations available

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