Bioconversion of Fumaric Acid to Succinic Acid by Recombinant E. coli

  • Xiaohai Wang
  • C. S. Gong
  • George T. Tsao
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


Succinic acid was produced efficiently from fumaric acid by a recombinant E. coli strain DH5α/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. l-Malic acid became the major fermentation product under these conditions. Provision of glucose (5–30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.

Index Entries

Recombinant E. coli fumarate reductase succinic acid fumaric acid 


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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Xiaohai Wang
    • 1
  • C. S. Gong
    • 1
  • George T. Tsao
    • 1
  1. 1.Laboratory of Renewable Resources Engineering1295 Potter Engineering Center, Purdue UniversityWest Lafayette

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