Bioconversion of Fumaric Acid to Succinic Acid by Recombinant E. coli

  • Xiaohai Wang
  • C. S. Gong
  • George T. Tsao
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Succinic acid was produced efficiently from fumaric acid by a recombinant E. coli strain DH5α/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. l-Malic acid became the major fermentation product under these conditions. Provision of glucose (5–30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.

Index Entries

Recombinant E. coli fumarate reductase succinic acid fumaric acid 

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References

  1. 1.
    Winstrom, L. O. (1983), in Kirk-Othmer Encyclopedia of Chemical Technology, vol. 21, Grayson, M. and Eckroth, D., eds., Wiley, New York, pp. 848–864.Google Scholar
  2. 2.
    Datta, R. (1992), US patent no. 5,143,833.Google Scholar
  3. 3.
    Zeikus, J. G., Elankovan, P., and Grethlein, A. (1995), Chem. Processing 58, 71–73.Google Scholar
  4. 4.
    Millard, C. S., Chao, Y.-P., Liao, J. C., and Donnelly, M. I. (1996), Appl. Environ. Microbiol. 62, 1808–1810.Google Scholar
  5. 5.
    Goldberg, I., Lonberg-Holm, K., Bagley, E. A., and Stieglitz, B. (1983), Appl. Environ. Microbiol. 45, 1838–1847.Google Scholar
  6. 6.
    Cao, N., Du, J., Gong, C. S., and Tsao, G. T. (1996), Appl. Environ. Microbiol. 62, 2926–2931.Google Scholar
  7. 7.
    Blaut, M., Whittaker, K., Valdovinos, A., Ackrell, B. A. C., Gunsalus, R. P., and Cecchini, G. (1989), J. Biol. Chem. 264, 13,599–13,604.Google Scholar
  8. 8.
    Van Hellemond, J. J. and Tielens, A. G. M. (1994), Biochem. J. 304, 321–331.Google Scholar
  9. 9.
    Hirsch, C. A., Rasminsky, M., Davis, B. D., and Lin, E. C. C. (1963), J. Biol Chem. 238, 3770–3774.Google Scholar
  10. 10.
    Cecchini, G., Ackrell, B. A. C., Kearney, E. B., and Gunsalus, R. P. (1984), in Flavins and Flavoproteins, Bray, R. C., Engel, P. C., and Mayhew, S. G., eds., Walter de Gruyter, New York, pp. 555–558.Google Scholar
  11. 11.
    Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Xiaohai Wang
    • 1
  • C. S. Gong
    • 1
  • George T. Tsao
    • 1
  1. 1.Laboratory of Renewable Resources Engineering1295 Potter Engineering Center, Purdue UniversityWest Lafayette

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