Fate of Branched-Chain Fatty Acids in Anaerobic Environment of River Sediment

  • H. Chua
  • W. Lo
  • P. H. F. Yu
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


The fate of six different branched-chain fatty acids (BCFAs) in an anaerobic environment of a river sediment was studied in vitro by culturing enrichment consortia. The anaerobic consortium of BCFA-degrading genus degraded BCFAs with tertiary carbons through β-oxidation, followed by methanogenesis by methane-producing anaerobic bacteria. The consortium could not degrade BCFAs with quaternary carbon. Degree of branching at the alpha or beta position along the carbon chain interfered with the beta-oxidation mechanisms of the branched-chain fatty acid.

Index Entries

Anaerobic consortium β-oxidation branched-chain fatty acid river sediment 


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  1. 1.
    Smith, C. R. (1970), Topics in Lipid Chemistry, Gunstone, F. D., ed., Logos, London, pp. 277–368.Google Scholar
  2. 2.
    Chua, H., Yap, M. G. S., and Ng, W. J. (1996), Water Research 30, 3007–3016.CrossRefGoogle Scholar
  3. 3.
    Yap, M. G. S., Relf, R. D., and Tan, S. B. (1990), Proc. Seminar on NUS-Industry Acheivements in R and D Collaboration. National University of Singapore, pp. 67–71.Google Scholar
  4. 4.
    Jimeno, A., Bermudez, J. J., Canovas-Diaz, M., Manjon, A., and Iborra, J. L. (1990), Biol. Wastes 34, 241–250.CrossRefGoogle Scholar
  5. 5.
    Masey, L. K., Sokatch, J., and Conrad, R. S. (1976), Bacteriol. Rev. 40, 42–54.Google Scholar
  6. 6.
    Chen, Y. F. (1993), Masters Thesis, National University of Singapore.Google Scholar
  7. 7.
    Ng, W. J., Yap, M. G. S., and Sivadas, M. (1989), Biol. Wastes. 29, 299–311.CrossRefGoogle Scholar
  8. 8.
    McInerney, M. J., Bryant, M. P., and Pfennig, N. (1979), Arch. Microbiol. 122, 129–135.CrossRefGoogle Scholar
  9. 9.
    McInerney, M. J., Bryant, M. P., Hespell, R. B., and Costerton, J. W. (1981), Appl. Env. Microbiol. 41, 1029–1039.Google Scholar
  10. 10.
    Chua, H., Yap, M. G. S., and Ng, W. J. (1992), Appl. Biochem. Biotechnol. 34/35, 789–800.CrossRefGoogle Scholar
  11. 11.
    Chua, H., and Chen, Y. F. (1995), Marine Pollut. Bull. 31, 313–316.CrossRefGoogle Scholar
  12. 12.
    Yap, M. G. S., Ng, W. J., and Chua, H. (1992), Bioresource Technol. 41, 45–51.CrossRefGoogle Scholar
  13. 13.
    Novak, J. T. and Carlson, D. A (1970), J. WPCF 42, 1932–1943.Google Scholar
  14. 14.
    Richardson, A. J., Hobson, P. N., and Campbell, G. P. (1987), Lett. Appl. Microbiol. 5, 119–121.CrossRefGoogle Scholar
  15. 15.
    Chua, H., Yap, M. G. S., and Ng, W. J. (1995), Appl. Biochem. Biotechnol. 51, 705–716.CrossRefGoogle Scholar
  16. 16.
    Drier, T. M. and Thurston, E. L. (1978), Scanning Electron Microscopy 11, 843–848.Google Scholar
  17. 17.
    Birk, G. (1984), Instrumentation and Techniques for Fluorescence Microscopy, Wild Leitz, Sydney, Australia.Google Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • H. Chua
    • 1
  • W. Lo
    • 2
  • P. H. F. Yu
    • 2
  1. 1.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityKowloonHong Kong
  2. 2.Department of Applied Biology and Chemical TechnologyHong Kong Polytechnic UniversityKowloonHong Kong

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