Advertisement

Myosin Light and Heavy Chains

  • Mauro Panteghini
Part of the Pathology and Laboratory Medicine book series (PLM)

Abstract

The basic building block of the contractile apparatus in skeletal and cardiac muscle cell is the sarcomere. This multisubunit structure is composed of a precise geometric arrangement of thick filaments sur-rounded by a hexagonal array of thin filaments, each containing actin and the tro-ponin-tropomyosin regulatory complex.

Keywords

Acute Myocardial Infarction Light Chain Heavy Chain Infarct Size Acute Myocardial Infarction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Murphy RA (1993) Muscle. In: Physiology, 3rd ed., Berne RM and Levy MN, eds., St. Louis, Mosby, pp. 284–287.Google Scholar
  2. 2.
    Matsuoka R, Chambers A, Kimura M, Kanda N, Bruns G, Yoshida M, and Takao A (1988) Molecular cloning and chromosomal localization of a gene coding for human cardiac myosin heavy-chain. Am. J. Med. Genet. 29:369–376.PubMedCrossRefGoogle Scholar
  3. 3.
    Jaenicke T, Diederich KW, Haas W, Schleich J, Lichter P, Pfordt M, Bach A, and Vos-berg HP (1990) The complete sequence of the human beta-myosin heavy chain gene and a comparative analysis of its product. Genomics 8:194–206.PubMedCrossRefGoogle Scholar
  4. 4.
    Liew CC, Sole MJ, Yamauchi-Takihara K, Kellam B, Anderson DH, Lin L, and Liew JC (1990) Complete sequence and organization of the human cardiac beta-myosin heavy chain gene. Nucleic Acid Res. 18:3647–3651.PubMedCrossRefGoogle Scholar
  5. 5.
    Matsuoka R, Beisel KW, Furutani M, Arai S, and Takao A (1991) Complete sequence of human cardiac alpha-myosin heavy chain gene and amino acid comparison to other myosins based on structural and functional differences. Am. J. Med. Genet. 41:537–547.PubMedCrossRefGoogle Scholar
  6. 6.
    Diederich KW, Eisele I, Ried T, Jaenicke T, Lichter P, and Vosberg HP (1989) Isolation and characterization of the complete human betamyosin heavy chain gene. Hum. Genet. 81:214–220.PubMedCrossRefGoogle Scholar
  7. 7.
    Mair J, Puschendorf B, and Michel G (1994) Clinical significance of cardiac contractile proteins for the diagnosis of myocardial injury. Adv. Clin. Chem. 31:63–98.PubMedCrossRefGoogle Scholar
  8. 8.
    Seidel U, Bober E, Winter B, Lenz S, Lohse P, and Arnold HH (1987) The complete nucleotide sequences of CDNA clones coding for human myosin light chains 1 and 3. Nucleic Acids Res. 15:4989.PubMedCrossRefGoogle Scholar
  9. 9.
    Hoffmann E, Shi QW, Floroff M, Mickle DAG, Wu TW, Olley PM, and Jackowski G (1988) Molecular cloning and complete nucleotide sequence of a human ventricular myosin light chain 1. Nucleic Acids Res. 16:2353.PubMedCrossRefGoogle Scholar
  10. 10.
    Bechet JJ and Houadjeto M (1989) Prediction of the secondary structure of myosin light chains from comparison of homologous sequences: implications for the interaction between myosin heavy and light chains. Biochim. Biophys. Acta. 996: 199–208.PubMedCrossRefGoogle Scholar
  11. 11.
    Wagner PD and Giniger E (1981) Hydrolysis of ATP and reversible binding of F-actin by myosin heavy chains free of all light chains. Nature 292:560–562.PubMedCrossRefGoogle Scholar
  12. 12.
    Samarel AM, Ferguson AG, Vander Heide RS, Davison R, and Ganote CE (1986) Release of unassembled rat cardiac myosin light chain 1 following the calcium paradox. Circ. Res. 58:166–171.PubMedCrossRefGoogle Scholar
  13. 13.
    Panteghini M (1992) Cardiac myosin light chains. Lab. Med. 23:318–322.Google Scholar
  14. 14.
    Smitherman TC, Dycus DW, and Richards EG (1980) Dissociation of myosin light chains and decreased myosin ATPase activity with acidification of synthetic myosin filaments: Possible clues to the fate of myosin in myocardial ischemia and infarction. J. Mol. Cell. Cardiol. 12:149–164.PubMedCrossRefGoogle Scholar
  15. 15.
    Leger JOC, Bouvagnet P, Pau B, Roncucci R, and Leger JJ (1985) Levels of ventricular myosin fragments in human sera after myocardial infarction, determined with monoclonal antibodies to myosin heavy chains. Eur. J. Clin. Invest. 15:422–429.PubMedCrossRefGoogle Scholar
  16. 16.
    Larue C, Calzolari C, Leger J, Leger J, and Pau B (1991) Immunoradiometric assay of myosin heavy chain fragments in plasma for investigation of myocardial infarction. Clin. Chem. 37:78–82.PubMedGoogle Scholar
  17. 17.
    Simeonova PP, Kehayov IR, and Kyurkchiev SD (1991) Identification of human ventricular myosin heavy chain fragments with monoclonal antibody 2F4 in human sera after myocardial necrosis. Clin. Chim. Acta. 201:207–222.PubMedCrossRefGoogle Scholar
  18. 18.
    Cardone B, Skea D, Yang J, and Jackowski G (1994) Development and characterization of monoclonal antibodies to human ventricular myosin heavy chain. Clin. Chem. 40:995.Google Scholar
  19. 19.
    Leger JOC, Larue C, Ming T, Calzolari C, Gautier P, Mouton C, Grolleau R, Louisot P, Puech P, Peperstraete B, Staroukine M, Telerman M, Pau B, and Leger JJ (1990) Assay of serum cardiac myosin heavy chain fragments in patients with acute myocardial infarction: Determination of infarct size and long-term follow-up. Am. Heart J. 120: 781–790.PubMedCrossRefGoogle Scholar
  20. 20.
    Seregni E, Luksch R, Crippa F, Bruni GF, and Bombardieri E (1994) Evaluation of serum osteocalcin and myosin in pediatric patients affected by osteosarcoma and rhabdomyosarcoma. Int. J. Biol. Markers 9:260–261.PubMedGoogle Scholar
  21. 21.
    Mair J, Koller A, Artner-Dworzak E, Haid C, Wicke K, Judmaier W, and Puschendorf B (1992) Effects of exercise on plasma myosin heavy chain fragments and MRI of skeletal muscle. J. Appl. Physiol. 72:656–663.PubMedGoogle Scholar
  22. 22.
    Gere JB, Krauth GH, Trahern CA, and Bigham DA (1979) A radioimmunoassay for the measurement of human cardiac myosin light chains. Am. J. Clin. Pathol. 71:309–318.PubMedGoogle Scholar
  23. 23.
    Katus HA, Yasuda T, Gold HK, Leinbach RC, Strauss HW, Waksmonski C, Haber E, and Knaw BA (1984) Diagnosis of acute myocardial infarction by detection of circulating cardiac myosin light chains. Am. J. Cardiol. 54:964–970.PubMedCrossRefGoogle Scholar
  24. 24.
    Isobe M, Nagai R, Ueda S, Tsuchimochi H, Nakaoka H, Takaku F, Yamaguchi T, Machii K, Nobuyoshi M, and Yazaki Y (1987) Quantitative relationship between left ventricular function and serum cardiac myosin light chain I levels after coronary reperfu-sion in patients with acute myocardial infarction. Circulation 76:1251–1261.PubMedCrossRefGoogle Scholar
  25. 25.
    Wang J, Shi Q, Wu TW, Jackowski G, and Mickle DAG (1989) The quantitation of human ventricular myosin light chain 1 in serum after myocardial necrosis and infarction. Clin. Chim. Acta. 181:325–336.PubMedCrossRefGoogle Scholar
  26. 26.
    Looser S, Hallermayer K, Gerber M, and Katus HA (1988) A new sensitive and specific enzyme immunoassay for human cardiac myosin light chains. Clin. Chem. 34:1273.Google Scholar
  27. 27.
    Hirayama A, Arita M, Takagaki Y, Tsuji A, Kodama K, and Inoue M (1990) Clinical assessment of specific enzyme immunoassay for the human cardiac myosin light chain II (MLC II) with use of monoclonal antibodies. Clin. Biochem. 23:515–522.PubMedCrossRefGoogle Scholar
  28. 28.
    Uji Y, Sugiuchi H, and Okabe H (1991) Measurement of human ventricular myosin light chain-1 by monoclonal solid-phase enzyme immunoassay in patients with acute myocardial infarction. J. Clin. Lab. Anal. 5:242–246.PubMedCrossRefGoogle Scholar
  29. 29.
    Katoh H, Sugi M, Chino S, Ishige M, Kuroda M, Fujimoto M, Nagai R, and Yasaki Y (1992) Development of an immunoradiometric assay kit for ventricular myosin light chain I with monoclonal antibodies. Clin. Chem. 38:170–171.PubMedGoogle Scholar
  30. 30.
    Wicks R, Vargas A, Wicks J, and Overdorf L (1992) Development of a solid phase EIA for the quantitation of ventricular myosin light chain 1 in human serum. Clin. Chem. 38:1094.Google Scholar
  31. 31.
    Michel G, Seifert B, and Ritter A (1992) Automated microparticle capture immunoassay for the measurement of human cardiac myosin light chain 1. Clin. Chem. 38:1104.Google Scholar
  32. 32.
    Nicol PD, Matsueda GR, Haber E, and Khaw BA (1993) Synthetic peptide immuno-gens for the development of a cardiac myosin light chain-1 specific radioimmunoassay. J. Nucl. Med. 34:2144–2151.PubMedGoogle Scholar
  33. 33.
    Ravkilde J, Botker HE, Sogaard P, Selmer J, Rej R, Jorgensen PJ, Horder M, and Thygesen K (1994) Human ventricular myosin light chain isotype 1 as a marker of myocardial injury. Cardiology 84:135–144.PubMedCrossRefGoogle Scholar
  34. 34.
    Bhayana V, Gougoulias T, Cohoe S, and Henderson AR (1995) Discordance between results for serum troponin T and troponin I in renal disease. Clin. Chem. 41:312–317.PubMedGoogle Scholar
  35. 35.
    Katus HA, Hurrell JG, Matsueda GR, Ehrlich P, Zurawski VR, Khaw BA, and Haber E (1982) Increased specificity in human car-diac-myosin radioimmunoassay utilizing two monoclonal antibodies in a double sandwich assay. Mol. Immunol. 19:451–455.PubMedCrossRefGoogle Scholar
  36. 36.
    Styba G, Takahashi M, Yang J, Davies E, Youn W, and Jackowski G (1994) Rapid format assay for the detection of cardiac myosin light chain-1 in patients with MI and unstable angina. Clin. Chem. 40:1021.Google Scholar
  37. 37.
    Svanas G, Choi W, Youn W, Leitmann R, Locicero P, Choi YH, Styba G, Kang J, and Jackowski G (1995) Measurement of cardiac myosin light chain 1 in a dry-strip format as an aid in the rapid diagnosis of myocardial injury. Clin. Chem. 41:S61.Google Scholar
  38. 38.
    Mair J, Wagner I, Jakib G, Lechleitner P, Dienstl F, Puschendorf B, and Michel G (1994) Different time courses of cardiac contractile proteins after acute myocardial infarction. Clin. Chim. Acta. 231:47–60.PubMedCrossRefGoogle Scholar
  39. 39.
    Mair J, Thome-Kromer B, Wagner I, Lechleitner P, Dienstl F, Puschendorf B, and Michel G (1994) Concentration time courses of troponin and myosin subunits after acute myocardial infarction. Coronary Artery Dis. 865–872.Google Scholar
  40. 40.
    Roberts R and Ishikawa Y (1983) Enzymatic estimation of infarct size during reperfusion. Circulation 68(Suppl. I):I183–I189.Google Scholar
  41. 41.
    Triggiani M, Simeone F, Gallorini C, Paolini G, Donatelli F, Paolillo G, Dolci A, and Grossi A (1994) Measurement of cardiac troponin T and myosin to detect periopera-tive myocardial damage during coronary surgery. Cardiovasc. Surg. 2:441–445.PubMedGoogle Scholar
  42. 42.
    Seguin JR, Saussine M, Fernere M, Leger JJ, Leger J, Lame C, Calzolari C, Grolleau R, and Chaptal PA (1989) Myosin: A highly sensitive indicator of myocardial necrosis after cardiac operations. J. Thorac. Cardiovasc. Surg. 98:397–401.PubMedGoogle Scholar
  43. 43.
    Leger JJ (1993) Plasma myosin, a marker of necrosis: An update and future trends. Arch. Mal. Coeur 86:29–32.PubMedGoogle Scholar
  44. 44.
    Nagai R, Ueda S, and Yazaki Y (1980) Radio-immunoassay of cardiac myosin light chain II in the serum following experimental myocardial infarction. Adv. Myocardiol. 2:415–420.PubMedGoogle Scholar
  45. 45.
    Nagai R, Chiu CC, Yamaoki K, Ueda S, Iwasaki Y, Ohkubo A, and Yasaki Y (1983) Serial changes in cytosolic, mitochondrial, and lyosomal enzymes and cardiac myosin light chain II in plasma following coronary ligation in conscious closed-chest dogs. Adv. Myocardiol. 4:473–478.PubMedGoogle Scholar
  46. 46.
    Nagai R and Yazaki Y (1981) Assessment of myocardial infarct size by serial changes in serum cardiac myosin light chain II in dogs. Jpn. Circ. J. 45:661–666.PubMedCrossRefGoogle Scholar
  47. 47.
    Trahern CA, Gere JB, Krauth GH, and Bigham DA (1978) Clinical assessment of serum myosin light chains in the diagnosis of acute myocardial infarction. Am. J. Car-diol. 41:641–645.Google Scholar
  48. 48.
    Panteghini M (1990) Asparate amino-transferase isoenzymes. Clin. Biochem. 23: 311–319.PubMedCrossRefGoogle Scholar
  49. 49.
    Nagai R, Chiu CC, Yamaoki K, Ohuchi Y, Ueda S, Imataka K, and Yazaki Y (1983) Evaluation of methods for estimating infarct size by myosin LC2: comparison with cardiac enzymes. Am. J. Physiol. 245: H413–H419.PubMedGoogle Scholar
  50. 50.
    Katus HA, Diederich KW, Uellner M, Remppis A, Schuler G, and Kubler W (1988) Myosin light chains release in acute myocardial infarction: non-invasive estimation of infarct size. Cardiovasc. Res. 22: 456–463.PubMedCrossRefGoogle Scholar
  51. 51.
    Mair J, Wagner I, Fridrich L, Lechleitner P, Dienstl F, Puschendorf B, and Michel G (1994) Cardiac myosin light chain-1 release in acute myocardial infarction is associated with scintigraphic estimates of myocardial scar. Clin. Chim. Acta. 229:153–159.PubMedCrossRefGoogle Scholar
  52. 52.
    Katus HA, Diederich KW, Schwarz F, Uellner M, Scheffold T, and Kubler W (1987) Influence of reperfusion on serum concentrations of cytosolic creatine kinase and structural myosin light chains in acute myocardial infarction. Am. Cardiol. 60:440–445.CrossRefGoogle Scholar
  53. 53.
    Yoshida H, Mochizuki M, Sakata K, Takezawa M, Matsumoto Y, Yoshimura M, Mori N, Yokoyama S, Hoshino T, and Kaburagi T (1992) Circulating myosin light chain I levels after coronary reperfusion: a comparison with myocardial necrosis evaluated from single photon emission computed tomography with pyrophosphate. Ann. Nucl. Med. 6:43–49.PubMedCrossRefGoogle Scholar
  54. 54.
    Hoberg E, Katus HA, Diederich KW, and Kubler W (1987) Myoglobin, creatine kinase-B isoenzyme, and myosin light chain release in patients with unstable angina pec-toris. Eur. Heart J. 8:989–994.PubMedGoogle Scholar
  55. 55.
    Katus HA, Diederich KW, Hoberg E, and Kubier W (1988) Circulating cardiac myosin light chains in patients with angina at rest: Identification of a high risk subgroup. J. Am. Coll. Cardiol. 11:487–493.PubMedCrossRefGoogle Scholar
  56. 56.
    Ravkilde J, Nissen H, Horder M, and Thygesen K (1995) Independent prognostic value of serum creatine kinase isoenzyme MB mass, cardiac troponin T and myosin light chain levels in suspected acute myocardial infarction. J. Am. Coll. Cardiol. 25: 574–581.PubMedCrossRefGoogle Scholar
  57. 57.
    Uchino T, Belboul A, Liu B, el-Gatit A, and Roberts D (1993) Detection of perioperative myocardial structural damage by the estimation of cardiac myosin light chain I. J. Cardiovasc. Surg. 34:517–522.Google Scholar
  58. 58.
    Uchino T, Belboul A, Roberts D, and Jagenburg R (1994) Measurement of myosin light chain I and troponin T as markers of myocardial damage after cardiac surgery. J. Cardiovasc. Surg. 35:201–206.Google Scholar
  59. 59.
    Uchino T, Belboul A, el-Gatit A, Roberts D, Berglin E, and William-Olsson G (1994) Assessment of myocardial damage by cir-culating cardiac myosin light chain I after heart transplantation. J. Heart Lung Transplant 13:418–423.PubMedGoogle Scholar
  60. 60.
    Kawauchi M, Gundry SR, Beierle F, de Begona JA, and Bailey LL (1993) Myosin light chain efflux after heart transplantation in infants and children and its correlation with ischemic preservation time. J. Thorac. Cardiavasc. Surg. 106:458–462.Google Scholar
  61. 61.
    Saitoh M, Miyakoda H, Kitamura H, Kasagi S, and Takamura H (1990) Clinical significance of serum cardiac myosin light chain 1 in patients with muscular dystrophy. Rinsho Shinkeigaku 30:835–839.PubMedGoogle Scholar
  62. 62.
    Fukunaga H, Higuchi I, Usuki F, Moritoyo T, and Okubo R (1992) Clinical significance of serum cardiac myosin light chain 1 in patients with Duchenne muscular dystrophy. No To Shinkei 44:131–135.PubMedGoogle Scholar
  63. 63.
    Mader R, Nicol PD, Turley ii, Bilbao J, and Keystone EC (1994) Inflammatory myopa-thy-early diagnosis and management by serum myosin light chains measurements. Isr. J. Med. Sci. 30:902–904.PubMedGoogle Scholar
  64. 64.
    Nakai K, Nakai K, Itoh C, Kikuchi M, Nakamura S, Kamata J, et al. (1992) Increased serum levels of human cardiac myosin light chain 1 in patients with renal failure. Rinsho Byori 40:529–534.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Mauro Panteghini

There are no affiliations available

Personalised recommendations