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Molecular Diagnostics

  • Dennis W. Ross
Chapter
  • 86 Downloads

Abstract

Molecular diagnostics seeks to detect errors that cause disease at the level of DNA. This is a rapidly evolving field with new technologies leapfrogging over previous methods. Unthinkable only a few years ago, now the most direct method of examining DNA is sequencing by automated methods. This chapter discusses the clinical laboratory tools available and probable near future developments. Surprisingly, I think that I will soon make apparent to you that our molecular technology is already well developed. Where we are stuck is limited clinical experience. Molecular diagnostics can detect disease at a level that we are only now ready to address. The major breakthroughs in molecular diagnostics await physicians discovering the correct use of the technology.

Keywords

Chronic Myeloid Leukemia Phase Fraction Molecular Diagnostics Image Cytometry Base Pair Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Fodor SPA. Massively parallel genomics. Science. 1997;277:393–395.CrossRefGoogle Scholar
  2. Hannan RE. Future practices in diagnostic medicine. Arch Pathol Lab Med 1995;119:890–893.PubMedGoogle Scholar
  3. Hess JL. Detection of chromosomal translocations in leukemia. Is there a best way? Am J Clin Pathol. 1998;109:3–5PubMedGoogle Scholar
  4. Lipshutz RJ, Morris D, Chee M, Hubbell E, Kozal MJ, Shah N, Shen N, Yang R, Fodor SPA. Using oligonucleotide probe arrays to access genetic diversity. Biotechniques. 1995;19:442–447.PubMedGoogle Scholar
  5. Ross DW. Clinical usefulness of DNA ploidy and cell cycle studies. Arch Pathol Lab Med 1993;117:1077.PubMedGoogle Scholar
  6. Sinclair PB, Green AR, Grace C, Nacheva EP. Improved sensitivity of BCRABL detection: a triple-probe three-color fluorescence in situ hybridization system. Blood. 1997;90:1395–1402.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Dennis W. Ross
    • 1
  1. 1.Department of PathologyForsyth Medical CenterWinston-SalemUSA

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