Lignin Peroxidase Production by Streptomyces viridosporus T7A

Use of Corn Oil as a Carbon Source
  • Leda M. F. Gottschalk
  • Jacyara M. B. Macedo
  • Elba P. S. Bon
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Lignin peroxidase (LiP) production cost should be reduced to justify its use in the control of environmental pollution. In this work, we studied the enzyme production by Streptomyces viridosporus T7A using glucose or corn oil as a carbon source having 0.65% yeast extract as a nitrogen source. Enzyme activity, observed using either 0.65% glucose or corn oil at 0.1, 0.5, and 1.0% concentration, was 300,150,300, and 200 UJL, respectively. Although higher enzyme activity was obtained in both media containing 0.65% glucose and 0.5% corn oil, the use of corn oil resulted in a better LiP stability. When combined carbon sources were used, higher values of enzyme activity (360, 350, and 225 UJL) were observed in media with 0.65% glucose and supplemented with 0.1,0.5, and 1.0% corn oil, respectively. Although the presence of both glucose and 0.5% corn oil is favorable for LiP production, satisfactory results in terms of enzyme production and stability could be also observed using 0.5% corn oil as a sole carbon source, which may lead to reduced production costs of the LiP enzyme.

Index Entries

Streptomyces viridosporus Lignin peroxidase enzyme production corn oil medium optimization 

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Leda M. F. Gottschalk
    • 1
  • Jacyara M. B. Macedo
    • 1
    • 2
  • Elba P. S. Bon
    • 1
  1. 1.Departamento de Bioquímica, IQ, CTUniversidade Federal do Rio de JaneiroBrazilUSA
  2. 2.Departamento de Bioquímica, IBRAGUniversidade do Estado do Rio de JaneiroBrazilUSA

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