Abstract
Bacillus subtilis S499 is well-known for its ability to produce two families of surfactant lipopeptides: Iturin A and Surfactin S1. Fermentation optimization for this strain was performed to amplify the surfactant production. Ten active variables were analyzed by two successive Plackett-Burman designs, consisting respectively of 12 and 16 experiments to give an optimized medium. The amount of biosurfactant lipopeptides in the supernatant of a culture carried out in this optimized medium was about five times higher than that obtained in nonoptimized rich medium. The analysis of the surfactant molecules produced in such optimized conditions has revealed the presence of a third family of lipopeptides: the fengycins.
The time-dependent production of these three families of molecules in bioreactors showed that surfactin SI is produced during the exponential phase and iturin A and fengycins during the stationary phase.
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© 1999 Springer Science+Business Media New York
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Jacques, P. et al. (1999). Optimization of Biosurfactant Lipopeptide Production from Bacillus subtilis S499 by Plackett-Burman Design. In: Davison, B.H., Finkelstein, M. (eds) Twentieth Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1604-9_22
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DOI: https://doi.org/10.1007/978-1-4612-1604-9_22
Publisher Name: Humana Press, Totowa, NJ
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