In Vitro Therapy for Sperm Motility: Specific and Nonspecific Treatments

  • Samir Hamamah
  • Geneviève Grizard
  • Alain Fignon
  • Sharon T. Mortimer
  • David Mortimer
Part of the Serono Symposia USA book series (SERONOSYMP)


Powerful assisted reproductive techniques (ART) have been developed to treat infertility. Intrauterine insemination (IUI) and in vitro fertilization (IVF) have been employed widely and allowed many couples the reward of children. With the improved ability to manipulate gametes precisely and routinely, intracytoplasmic sperm injection (ICSI) became possible and today represents one of the greatest technological advances in our capacity to treat various forms of infertility due to severe sperm defects. This highly technical treatment, however, is still not available in all ART centers and has been suggested to have a major drawback in that it bypasses the roocyte’s natural barriers that might discriminate spermatozoa before fertilization.


Hyaluronic Acid Sperm Motility Follicular Fluid Seminal Plasma Human Sperm 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Kanwar KC, Yanagimachi R, Lopata A. Effects of human seminal plasma on fertilizing capacity of human spermatozoa. Fertil Steril 1976;31:321–27.Google Scholar
  2. 2.
    Rogers BJ, Bastias C, Russell LD, Peterson RN. Cytochalasin-D inhibition of guinea pig sperm actin reduces fertilization. Ann N Y Acad Sci 1988;513:566–68.Google Scholar
  3. 3.
    Mortimer ST. A critical review of the physiological importance and analysis of sperm movement in mammals. Hum Reprod Update 1997;3:403–39.PubMedGoogle Scholar
  4. 4.
    ESHRE Andrology Special Interest Group. Guidelines on the application of CASA technology in the analysis of spermatozoa. Hum Reprod 1998;13:142–45.Google Scholar
  5. 5.
    Holt WW, Moore HDM, Hillier SG. Computer-assisted measurement of sperm swimming speed in human semen: correlation of results with in vitro fertilization assays. Fertil Steril 1985;44:112–19.PubMedGoogle Scholar
  6. 6.
    Hinting A, Comhaire F, Vermeulen L, Dhont M, Vermeulen A, Vandekerckhove D. Value of sperm characteristics and results of in vitro fertilization for predicting the outcome of assisted reproduction. J Androl 1989;13:59–66.Google Scholar
  7. 7.
    Grunert JH, De Geyter C, Bordt J, Schneider HPG, Neischlag E. Does computerized image of sperm movement enhance the predictive value of semen analysis for in vitro fertilization results?. Int J Androl 1989;12:329–38.PubMedGoogle Scholar
  8. 8.
    Jeulin C, Feneux D, Serres C, et al. Sperm factors related to failure of human in vitro fertilization. J Reprod Fertil 1986;76:735–44.PubMedGoogle Scholar
  9. 9.
    Chan SYW, Wang C, Chan STH, et al. Predictive value of sperm morphology and movement characteristics in the outcome of in vitro fertilization of human oocytes. J In Vitro Fertil Embryo Trans 1989;6:142–48.Google Scholar
  10. 10.
    Bongso TA, Ng SC, Mok H, et al. Effect of sperm motility on human in vitro fertilization. Arch Androl 1989;22:185–90.PubMedGoogle Scholar
  11. 11.
    Oehninger S, Acosta R, Kruger T, et al. Relationship between morphology and motion characteristics of human spermatozoa in semen and the swim-up fractions. J Androl 1990;11:446–52.PubMedGoogle Scholar
  12. 12.
    Gerris J, Khan I. Correlation between in vitro fertilization and human sperm density and motility. JAndrol 1987;8:48–53.Google Scholar
  13. 13.
    Talbert LM, Hammond MG, Halme J, O’Rand M, Fryer JG, Ekstrom RD. Semen parameters and fertilization of human oocytes in vitro: a multivariable analysis. Fertil Steril 1987;48:270–77.PubMedGoogle Scholar
  14. 14.
    Yanagimachi R. In vitro capacitation of hamster spermatozoa by follicular fluid. J Reprod Fertil 1969;18:275–86.PubMedGoogle Scholar
  15. 15.
    Barlow P, Devligne A, Van-Dromme J, Van-Hoeck J, Vandenbosch K, Leroy F. Predictive value of classical and automated sperm analysis for in vitro fertilization. Hum Reprod 1992;6:1119–24.Google Scholar
  16. 16.
    Hinney B, Wilke G, Michelmann HW. Prognostic value of an automated sperm analysis in IVF or insemination therapy. Andrologia 1993;25:195–202.PubMedGoogle Scholar
  17. 17.
    Ford WCL, Rees JM. The bioenergetics of mammalian sperm motility. In: Gagnon C, editor. Controls of sperm motility: biological and clinical aspects. Boca Raton, FL: CRC Press, 1990:175–202.Google Scholar
  18. 18.
    Gwatkin RBL, Anderson OF. Capacitation of hamster spermatozoa by bovine follicular fluid. Nature 1969;224:1111–12.PubMedGoogle Scholar
  19. 19.
    Burkman LJ. Characterization of hyperactivated motility by human spermatozoa during capacitation: comparison of fertile and oligozoospermic sperm populations. ArchAndrol 1984;13:153–65.Google Scholar
  20. 20.
    Mortimer D, Courtot AM, Giovangrandi Y, Jeulin C, David G. Human sperm motility after migration into, and incubation in synthetic media. Gamete Res 1984;9:131–44.Google Scholar
  21. 21.
    Katz DF, Yanagimachi R. Movement characteristics of hamster spermatozoa within the oviduct. Biol Reprod 1980;22:759–64.PubMedGoogle Scholar
  22. 22.
    Demott RP, Suarez SS. Hyperactivated sperm progress in the mouse oviduct. Biol Reprod 1992;45:779–85.Google Scholar
  23. 23.
    Fraser L. Dibutyryl cyclic AMP decreases capacitation time in vitro in mouse spermatozoa. J Reprod Fertil 1981;62:63–72.PubMedGoogle Scholar
  24. 24.
    Katz DF, Cherr GN, Lambert H. The evolution of hamster sperm motility during capacitation and interaction with the ovum investments in vitro. Gamete Res 1986;14:333–6.Google Scholar
  25. 25.
    Wang C, Lee GS, Leung A, Surrey ES, Chan SYW. Human sperm hyperactivation and acrosome reaction and their relationships to human in vitro fertilization. Fertil Steril 1993;59:1221–27.PubMedGoogle Scholar
  26. 26.
    Ord T, Patrizio P, Marello E, Balmaceda JP, Asch RH. Mini-Percoll: a new method of semen preparation for in vitro fertilization in severe male factors infertility. Hum Reprod 1990;5:987–89.PubMedGoogle Scholar
  27. 27.
    Mortimer D. Sperm preparation techniques and iatrogenic failures of in vitro fertilization. Hum Reprod 1991;6:173–76.PubMedGoogle Scholar
  28. 28.
    Forster MS, Smith WD, Lee WI, Berger RE, Karp LE, Stenchever MA. Selection of human spermatozoa according to their relative motility and their interaction with zona-free hamster eggs. Fertil Steril 1983;40:655–65.PubMedGoogle Scholar
  29. 29.
    Kossakowski J, Morrison L, Mortimer D. Evaluation of PureSperm gradients instead of Percoll for human spermatozoa. Hum Reprod 1997;12:88 (Abs).Google Scholar
  30. 30.
    Berger T, Marrs RP, Moyer DL. Comparison of techniques for selection of motile spermatozoa. Fertil Steril 1985;43:268–73.PubMedGoogle Scholar
  31. 31.
    Hyne RV, Stojanoff A, Clarke GN, Lopata A, Johnston WIH. Pregnancy from in vitro fertilization of human eggs after separation of motile sperm by density gradient centrifugation. Fertil Steril 1986;45:93–96.PubMedGoogle Scholar
  32. 32.
    Guérin JF, Mathieu C, Lornage J, Pinatel MC, Boulieu D. Improvement of survival and fertilizing capacity of human spermatozoa in an IVF programme by selection on discontinuous Percoll gradients. Hum Reprod 1989;4:798–804.PubMedGoogle Scholar
  33. 33.
    Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Fertil Reprod 1987;81:459–69.Google Scholar
  34. 34.
    Russell LD, Rogers BJ. Improvement in the quality and fertilization potential of a human sperm population using the rise technique. J Androl 1987;8:25–30.PubMedGoogle Scholar
  35. 35.
    Wikland M, Wiko O, Quist K, Soderlund B, Janson PO. A self migration method for preparation of sperm for in vitro fertilization. Hum Reprod 1987;2:191–95.PubMedGoogle Scholar
  36. 36.
    Ng FLH, Liu DY, Gordon Baker HW. Comparison of Percoll, mini-Percoll and swim-up methods for sperm preparation from abnormal semen samples. Hum Reprod 1992;7:261–66.PubMedGoogle Scholar
  37. 37.
    Perez SM, Chan PJ, Patton WC, King A. Silane coated silica particle colloid processing of human sperm. J Assist Reprod Genet 1997;14:388–93.PubMedGoogle Scholar
  38. 38.
    Van-den-Bergh M, Revelard P, Bertrand E, Birmane J, Vanin AS, Englert Y. Glass wool column filtration an advantageous way of preparing semen samples for intra-cytoplasmic sperm injection: an auto-controlled randomized study. Hum Reprod 1997;12:509–13.PubMedGoogle Scholar
  39. 39.
    Johnson DE, Confïno E, Jeyendran RS. Glass wool column filtration versus mini Percoll gradient for processing poor quality semen samples. Fertil Steril 1996;66:459–62.PubMedGoogle Scholar
  40. 40.
    Zavos PM, Correa JR, Sofikitis N, Kofinas GD, Zarmakoupis PN. A method of short term cryostorage and selection of viable sperm for use in the various assisted reproductive techniques. Tohoku J Exp Med 1995;176:75–81.PubMedGoogle Scholar
  41. 41.
    Smith S, Hosid S, Scott L. Use of postseparation sperm parameters to determine the method of choice for sperm preparation for assisted reproductive technology. Fertil Steril 1996;63:591–97.Google Scholar
  42. 42.
    Yamamoto Y, Maenosono S, Okada H, Miyagawa I, Sofikitis N. Comparisons of sperm quality, morphometry and function among human sperm populations recovered via SpermPrep II filtration, swim-up and Percoll density gradient methods. Andrologia 1997;29:303–10.PubMedGoogle Scholar
  43. 43.
    Makler A, Fisher M, Murillo O, Laufer N, DeCherney A, Naftolin F. Factors affecting sperm motility. IX. Survival of spermatozoa in various biological media and under different gaseous compositions. Fertil Steril 1984;41:428–32.PubMedGoogle Scholar
  44. 44.
    Soldati G, Piffaretti-Yanez A, Campana A, Marchini M, Luerti M, Baierna M. Effect of peritoneal fluid on sperm motility and velocity distribution using objective measurements. Fertil Steril 1989;52:113–19.PubMedGoogle Scholar
  45. 45.
    Suarez SS, Wolf DP, Meizel S. Induction of the acrosome reaction in human spermatozoa by a fraction of human follicular fluid. Gamete Res 1986;14:107–21.Google Scholar
  46. 46.
    Mbizvo M, Burkman LJ, Alexender NJ. Human follicular fluid stimulates hyperactivated motility in human sperm. Fertil Steril 1990;54:708–12.PubMedGoogle Scholar
  47. 47.
    Mendoza C, Tesarik J. Effect of follicular fluid on sperm movement characteristics. Fertil Steril 1990;54:1134–39.Google Scholar
  48. 48.
    Siegel MS, Paulson RJ, Graczykowski JW. The influence of human follicular fluid on the aerosome reaction, fertilizing capacity and proteinase activity of human spermatozoa. HumReprod 1990;5:975–80.Google Scholar
  49. 49.
    Falcone L, Gianni S, Piffaretti-Yanez A, Marchini M, Eppenberger U, Baierna M. Follicular fluid enhances sperm motility and velocity in vitro. Fertil Steril 1991;55:619–26.PubMedGoogle Scholar
  50. 50.
    Hamamah S, Chevrier C, Royere D, Lansac J, Gatti JL, Dacheux JL. Follicular fluid: effects on survival/motility of human spermatozoa. Assist Reprod Technol Androl 1992;3:75–80.Google Scholar
  51. 51.
    Hamamah S, Lanson M, Barthélémy C, Garrigue MA, Lansac J, Royere D. Treatment of human spermatozoa with follicular fluid can influence the lipid content and the motility during in vitro capacitation. Reprod Nutr Dev 1993;33:429–35.PubMedGoogle Scholar
  52. 52.
    Fabiani R, Johanson L, Lundkvist Ö, Ronquist G. Enhanced recruitment of motile spermatozoa by prostasome inclusion in swim-up medium. Hum Reprod 1994;9:1485–89.PubMedGoogle Scholar
  53. 53.
    Fabiani R, Johanson L, Lundkvist Ö, Ulmsten U, Ronquist G. Promotive effect by prostasomes on normal human spermatozoa exhibiting no forward motility due to buffer washings. Eur J Obstet Gynecol Reprod Biol 1994;57:181–88.PubMedGoogle Scholar
  54. 54.
    Aitken RJ, Kelly RW. Analysis of the direct effects of prostaglandins on human sperm function. J Reprod Fertil 1985;73:139–46.PubMedGoogle Scholar
  55. 55.
    Colon JM, Ginsburg F, Lessing JB, et al. The effect of relaxin and prostaglandin E2 on the motility of human spermatozoa. Fertil Steril 1986;46:1133–39.PubMedGoogle Scholar
  56. 56.
    Uhler M, Leung A, Chan SYW, Wang C. Direct effects of progesterone and antiprogesterone on human sperm hyperactivated motility and aerosome reaction. Fertil Steril 1992;58:1191–98.PubMedGoogle Scholar
  57. 57.
    Schill WB, Haberland GL. Kinin induced enhancement of sperm motility. Hoppe-Seyler′s Z Physiol Chem 1974;355:229–31.PubMedGoogle Scholar
  58. 58.
    Sato H, Hchill WB. Temperature dependent effects of the components of kal-likrein-kinin system on sperm motility in vitro. Fertil Steril 1987;47:684–88.PubMedGoogle Scholar
  59. 59.
    Hammitt DG, Bedia E, Rogers PR, Syrop CH, Donovaan JF, Williamson RA. Comparison of motility stimulants for cryopreserved human semen. Fertil Steril 1989;52:495–502.PubMedGoogle Scholar
  60. 60.
    Ricker DD, Minhas BS, Kumar R, Robertson JL, Dodson MG. The effects of platelet activating factor on the motility of human spermatozoa. Fertil Steril 1989;52:655–58.PubMedGoogle Scholar
  61. 61.
    Wang R, Sikka SC, Veeraragavan K, Bell M, Hellstrom WJG. Platelet activating factor and pentoxifylline as human sperm cryoprotectants. Fertil Steril 1993;60:711–15.PubMedGoogle Scholar
  62. 62.
    Fakih H, MacLusky N, DeCherney A, Wallimann T, Huszar G. Enhancement of human sperm motility and velocity in vitro: effects of calcium and creatine phosphate. Fertil Steril 1986;46:938–44.PubMedGoogle Scholar
  63. 63.
    Pilikian S, Adeleine P, Czyba JC, Ecochard R, Guerin JF, Mimouni P. Hyperactivated motility of sperm from fertile donors and asthenozoospermic patients before and after treatment with ionophore. Int J Androl 1991;14:167–73.PubMedGoogle Scholar
  64. 64.
    Hong CY, Chiang BN, Ku J, Wei YH. Calcium chelators stimulate sperm motility in ejaculated human semen. Lancet 1984;i:460–61.Google Scholar
  65. 65.
    Huazar G, Willetts M, Corrales M. Hyaluronic acid (sperm Select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril 1990;54:1 127–34.Google Scholar
  66. 66.
    Hamamah S, Wittemer CH, Barthélémy C, et al. Identification of hyaluronic acid and chondroitin sulfates in human follicular fluid and their effects on human sperm motil-ity and the outcome in vitro fertilization. Reprod Nutr Dev 1996;36:43–52.PubMedGoogle Scholar
  67. 67.
    Aitken RJ, Mattei A, Irvine S. Paradoxical stimulation of human sperm motility by 2-deoxyadenosine. J Reprod Fertil 1986;78:515–27.PubMedGoogle Scholar
  68. 68.
    Imoedemhe DAG, Sigue AB, Pacpaco ELA, Olazo AB. Successful use of the sperm motility enhancer 2-deoxyadenosine in previously failed human in vitro fertilization. v J Assist Reprod Genet 1992;9:53–56.Google Scholar
  69. 69.
    Imoedemhe DAG, Sigue AB, Pacpaco ELA, Olazo AB. In vitro fertilization and embryonic development of oocytes fertilized by sperm treated with 2-deoxyadenosine. Int J Fertil 1993;38:235–40.Google Scholar
  70. 70.
    Mbizvo MT, Johnston RC, Baker GHW. The effect of the motility stimulants caffeine, pentoxifylline and 2-deoxyadenosine on hyperactivation of cryopreserved human sperm. Fertil Steril 1993;59:1112–17.PubMedGoogle Scholar
  71. 71.
    Yee B, Cummings LM. Modification of the sperm penetration assay using human follicular fluid to minimize false negative results. Fertil Steril 1988;50:123–28.PubMedGoogle Scholar
  72. 72.
    Ghetler Y, Ben-Nun I, Kaneti H, Jaffe R, Gruber A, Fejgin M. Effect of preincubation with follicular fluid on the fertilization rate in human in vitro fertilization. Fertil Steril 1990;54:944–46.PubMedGoogle Scholar
  73. 73.
    Blackmore PF, Beebe SJ, Danforth DR, Alexander N. Progesterone and 17 a-hydroxyprogesterone. Novel stimulators of calcium influx in human sperm. J Biol Chem 1990;25:1376–80.Google Scholar
  74. 74.
    Kulin S, Bastiaans BA, Hollanders HMG, Janssen HJG, Goverde HJM. Human serum and follicular fluid stimulate hyperactivation of human spermatozoa after preincubation. Fertil Steril 1994;62:1234–37.PubMedGoogle Scholar
  75. 75.
    Kopf GS, Kalab P, Leclerc P, Ning XP, Pan D, Visconti P. Signal transduction in mammalian spermatozoa. In: Verhoen G, Habenicht U-F, editors. Molecular and cellular endocrinology of the testis. Berlin: Springer, 1994:153–83.Google Scholar
  76. 76.
    Thomas P, Meizel S. An influx of extracellular calcium is required for initiation of the human sperm acrosome reaction induced by human follicular fluid. Gamete Res 1988;20:397–411.PubMedGoogle Scholar
  77. 77.
    Tesarik J, Mendoza C, Moos J, Fénichel P, Fehlmann M. Progesterone action through aggregation of a receptor on the sperm plasma membrane. FEBS Lett 1992;308:116–20.PubMedGoogle Scholar
  78. 78.
    Robert M, Gagnon C. Purification and characterization of the active precursor of a human sperm motility inhibitor secreted by the seminal vesicles: identity with semenogelin. Biol Reprod 1996;55:813–21.PubMedGoogle Scholar
  79. 79.
    Arvidson G, Ronquist G, Wikander G, Öjteg AC. Human prostasomes membranes exhibit very high cholesterol/phospholipid ratio yielding high molecular ordering. Biochim Biophys Actal 1984:167–73.Google Scholar
  80. 80.
    Ronquist G, Nilsson BO, Hjerten S. Interaction between prostasomes and spermatozoa from human semen. Arch Androl 1990;24:147–57.PubMedGoogle Scholar
  81. 81.
    Arienti G, Carlini E, Palmerini CA. Fusion of human sperm to prostasomes at acidic pH. J Membr Biol 1997;155:89–94.PubMedGoogle Scholar
  82. 82.
    Carlini E, Palmerini CA, Cosmi EV, Arienti G. Fusion of sperm with prostasomes: effects on membrane fluidity. Arch Biochem Biophys 1997;343:6–12.PubMedGoogle Scholar
  83. 83.
    Stegmayr B, Ronquist G. Stimulation of sperm progressive motility by organelles in human seminal plasma. Scand J Urol Nephrol 1982;16:85–90.PubMedGoogle Scholar
  84. 84.
    Fabiani R, Johanson L, Lundkvist Ö, Ronquist G. Prolongation and improvement of prostasome promotive effect on sperm forward motility. Eur J Obstet Gynecol Reprod Biol 1995;58:191–98.PubMedGoogle Scholar
  85. 85.
    Lenz RW, Ax RL, Grimek HJ, First NL. Proteoglycan from bovine follicular fluid stimulates an aerosome reaction in bovine spermatozoa. Biochem Biophys Res Commun 1982;106:1092–98.PubMedGoogle Scholar
  86. 86.
    Eriksen GV, Malmström A, Carlstedt I, Uldbjerg N. Human follicular fluid contains two large proteoglycans. In: Leppert PC, Woessner F, editors. The extracellular matrix of the uterus, cervix and fetal membranes: synthesis, degradation and hormonal regulation. Ithaca, New York: Perinatology Press, 1991:274–78.Google Scholar
  87. 87.
    Eriksen GV, Malmström A, Uldbjerg N, Huszar G. A follicular fluid chondroitin sulfate proteoglycan improves the retention of motility and velocity of human spermatozoa. Fertil Steril 1994;62:618–23.PubMedGoogle Scholar
  88. 88.
    Karlstrom PO, Bakos O, Bergh T, Lundkvist O. Intrauterine insemination and comparison of two methods of sperm preparation. Hum Reprod 1991;6:390–95.PubMedGoogle Scholar
  89. 89.
    Huazar G, Willetts M, Corrales M. Hyaluronic acid (sperm Select) improves retention of sperm motility and velocity in normospermic and oligospermic specimens. Fertil Steril 1990;54:1127–34.Google Scholar
  90. 90.
    Kornovski BS, McCoshen J, Kredentser J, Turley E. The regulation of sperm motility by a novel hyaluronan receptor. Fertil Steril 1994;61:935–40.PubMedGoogle Scholar
  91. 91.
    Ranganathan S, Ganauly AK, Datta K. Evidence for presence of hyaluronan binding protein on spermatozoa and its possible involvement in sperm function. Mol Reprod Dev 1994;38:69–76.PubMedGoogle Scholar
  92. 92.
    Yanagimachi R. Mammalian fertilization. In: Knobil E, Neill JD, Ewing L, Market CL, Greenwald GS, Pfaff DW, editors. The physiology of reproduction. New York: Raven Press, 1994:189–221.Google Scholar
  93. 93.
    Vijarayaghavan S, Trautman KD, Goueli SA, Carr DW. A tyrosine-phosphorylated 55-kDa motility associated bovine sperm protein is regulated by cAMP and calcium. Biol Reprod 1997;56:1450–57.Google Scholar
  94. 94.
    Barkay J, Zuckerman H, Sklan D, Gordon S. Effect of caffeine on increasing the motility of frozen human sperm. Fertil Steril 1977;28:175–77.PubMedGoogle Scholar
  95. 95.
    Sikka SC, Hellstrom WJG. The application of pentoxifylline in the stimulation of sperm motion in men undergoing electroejaculation. J Androl 1991;12:165–70.PubMedGoogle Scholar
  96. 96.
    Paul M, Sumpter JP, Lindsay KS. The paradoxical effects of pentoxifylline on the binding of spermatozoa to the human zona pellucida. Hum Reprod 1996;11:814–19.PubMedGoogle Scholar
  97. 97.
    Lanzafame F, Chapman MG, Guglielmino A, Gearon CM, Forman RG. Pharmacological stimulation of sperm motility. Hum Reprod 1994;9:192–99.PubMedGoogle Scholar
  98. 98.
    Matson PHL, Yovich JM, Edirisinghe WR, Junk SM, Yovich JL. An argument for the past and continued use of pentoxifylline in assisted reproductive technology. Hum Reprod 1995;10(Suppl 1):67–71.PubMedGoogle Scholar
  99. 99.
    Tournaye H, Devroey P, Camus M, Van-der-Linden M, Janssens R, Van-Steirteghem A. Use of pentoxifylline in assisted reproductive technology. Hum Reprod 1995;10(Suppll):72–79.PubMedGoogle Scholar
  100. 100.
    Schill WB. Survey of medical therapy in andrology. Int J Androl 1995;18 (Suppl2):56–62.PubMedGoogle Scholar
  101. 101.
    Merino G, Martinez-Chequer JC, Barahona E, et al. Effects of pentoxifylline on sperm motility in normogonadotrophic asthenozoospermic men. Arch Androl 1997;39:65–69.PubMedGoogle Scholar
  102. 102.
    McKinney KA, Lewis SE, Thompson W. The effects of pentoxifylline on the generation of reactive oxygen species and lipid perioxidation in human spermatozoa. Andrologia 1996;28:15–20.PubMedGoogle Scholar
  103. 103.
    Okada H, Tatsumi N, Kanzaki M, Fujisawa M, Arakawa S, Kamidono S. Formation of reactive oxygen species by spermatozoa from asthenospermic patients: response to treatment with pentoxifylline. J Urol 1997;157:2140–46.PubMedGoogle Scholar
  104. 104.
    Nivsarkar M, Patel RY, Mokal R. Modulation of sperm membrane conformation by pentoxifylline in oligospermia: a biophysical investigation of sperm membrane in vitro. Bichem Biophys Res Commun 1996;225:791–95.Google Scholar
  105. 105.
    Tesarik J, Thébault A, Testart J. Effect of pentoxifylline on sperm movement characteristics in normozoospermic and asthenozoospermic specimens. Hum Reprod 1992;7:1257–63.PubMedGoogle Scholar
  106. 106.
    Pang SC, Chan PJ, Lu A. Effects of pentoxifylline on sperm motility and hyperactivation in normozoospermic and normokinetic semen. Fertil Steril 1993;60:336–43.PubMedGoogle Scholar
  107. 107.
    De Lamirande E, Gagnon C. A positive role for Superoxide anion in triggering hyperactivation and capacitation of human spermatozoa. Int J Androl 1993;16:21–25.PubMedGoogle Scholar
  108. 108.
    Aitken J, Fisher H. Reactive oxygen species generation and human spermatozoa: the balance of benefit and risk. Bioessays 1994;16:259–67.PubMedGoogle Scholar
  109. 109.
    Suleiman SA, Ali ME, Zaki ZM, el Malik EM, Nasr MA. Lipid perioxidation and human sperm motility: protective role of vitamin E. J Androl 1996;17:530–37.PubMedGoogle Scholar
  110. 110.
    Geva E, Bartoov B, Zabludovsky N, Lessing JB, Lerner-Geva L, Amit A. The effect of antioxidant treatment on human spermatozoa and fertilization rate in an in vitro fertilization program. Fertil Steril 1996;66:430–34.PubMedGoogle Scholar
  111. 111.
    Moilanen J, Hovatta O. Excretion of alpha-tocopherol into human seminal plasma after oral administration. Andrologia 1995;27:133–36.PubMedGoogle Scholar
  112. 112.
    Therond P, Auger J, Legrand A, Jouannet P. Alpha-tocopherol in human spermatozoa and seminal plasma: relationships with motility, antioxidant enzymes and leukocytes. Mol Hum Reprod 1996;10:739–44.Google Scholar
  113. 113.
    Palan P, Naz R. Changes in various antioxidant levels in human seminal plasma related to immunoinfertility. Arch Androl 1996;36:139–43.PubMedGoogle Scholar
  114. 114.
    Askari HA, Check JH, Peymer N, Bollendorf A. Effect of natural antioxidants toco-pherol and ascorbic acids in maintenance of sperm activity during freeze-thaw process. Arch Androl 1994;33:11–15.PubMedGoogle Scholar
  115. 115.
    Brezezinska-Slebodzinska E, Slebodzinski AB, Pietras B, Wieczorek G. Antioxidant effect of vitamin E and glutathione on lipid perioxidation in boar semen plasma. Biol Trace Elem Res 1995;47:69–74.PubMedGoogle Scholar
  116. 116.
    Lewis SE, Donnelly ET, Sterling ES, Kennedy MS, Thompson W, Chakravarthy U. Nitric oxide synthase and nitrite production in human spermatozoa: evidence that endogenous nitritic oxide is beneficial to sperm motility. Mol Hum Reprod 1996;2:873–78.PubMedGoogle Scholar
  117. 117.
    Zini A, De Lamirande E, Gagnon C. Low levels of nitric oxide promote human sperm capacitation in vitro. J Androl 1995;16:424–31.PubMedGoogle Scholar
  118. 118.
    Rosselli M, Dubey RK, Imthur B, Macas E, Keller PJ. Effects of nitric oxide on human spermatozoa: evidence that nitric oxide decreases sperm motility and induces sperm toxicity. Hum Reprod 1995;10:1786–90.PubMedGoogle Scholar
  119. 119.
    Nabunaga T, Tokugawa Y, Hashimoto K, et al. Elevated nitric oxide concentration in the seminal plasma of infertile males: nitric oxide inhibits sperm motility. Am J Reprod Immunol 1996;36:193–97.Google Scholar
  120. 120.
    Zhang H, Zheng RL. Possible role of nitric oxide on fertile and asthenozoospermic infertile human sperm functions. Free Radic Res 1996;25:347–57.PubMedGoogle Scholar
  121. 121.
    Szabo C, Southan GJ, Wood E, Thiemermann C, Vane JR. Inhibition by spermine of the induction of nitric oxide synthase in J774.2 macrophages: requirement of a serum factor. Br J Pharmacol 1994;112:355–56.PubMedGoogle Scholar
  122. 122.
    Ruzich JV, Gill H, Wein AJ, Van Arsdalen K, Hypolite J, Levin RM. Objective assessment of the effect of caffeine on sperm motility and velocity. Fertil Steril 1987;48:891–93.PubMedGoogle Scholar
  123. 123.
    Rogberg L, Fredricsson B, Poussette A. Effects of propanolol and caffeine on movement characteristics of human sperm. Int J Androl 1990;13:87–92.PubMedGoogle Scholar
  124. 124.
    Sikka SC, Hellstrom WJC. Functional evaluation and motility parameters of pentoxifylline-stimulated cryopreserved human sperm. Assist Reprod Technol Androl 1990;1:309–19.Google Scholar
  125. 125.
    Kay VJ, Courts JRT, Robertson L. Pentoxifylline stimulates hyperactivation in human spermatozoa. Hum Reprod 1993;8:727–31.PubMedGoogle Scholar
  126. 126.
    Fuse H, Sakamoto M, Ohta S, Katayama T. Effect of pentoxifylline on sperm motion. ArchAndrol 1993;31:9–15.Google Scholar
  127. 127.
    Lewis SEM, Moohan J, Thompson W. Effect of pentoxifylline on human sperm motility in normospermic individuals using computer assisted analysis. Fertil Steril 1993;59:418–23.PubMedGoogle Scholar
  128. 128.
    Aribarg A, Sukcharoen N, Jetsawangsri U, Chanprasit Y, Ngeamvijawat J. Effects of pentoxifylline on sperm motility characteristics and motility longevity of post-thaw cryopreserved semen using computer assisted semen analysis. J Med Assoc Thai 1994;77:71–75.PubMedGoogle Scholar
  129. 129.
    Brennan AP, Holden CA. Pentoxifylline supplemented cryoprotectant improves human sperm motility after cryoconservation. Hum Reprod 1995;10:2308–12.PubMedGoogle Scholar
  130. 130.
    Tarlatzis BC, Kolibianakis EM, Bontis J, Tousiou M, Lagos S, Mantalenakis S. Effect of pentoxifylline on human sperm motility and fertilizing capacity. Arch Androl 1995;34:33–42.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Samir Hamamah
  • Geneviève Grizard
  • Alain Fignon
  • Sharon T. Mortimer
  • David Mortimer

There are no affiliations available

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