Abstract
Microinsemination (MI), also called microassisted fertilization or microfertilization, has been used extensively in clinical practice and for research purposes. The first attempt to fertilize mammalian oocytes by MI was made by Uehara and Yanagimachi in 1976 (1). They demonstrated that injected sperm heads could develop into pronuclei after incorporation into homologous and heterologous oocytes. Since then MI with several mammalian species has increased our fundamental knowledge of fertilization. Full-term embryo development following in-tracytoplasmic sperm injection (ICSI) was first demonstrated in the rabbit (2), followed by bovines (3). Even though the efficiency of ICSI in these animal studies was disappointingly low, human ICSI proved to be very efficient and became the method of choice to overcome male factor infertility (4). The history and technical aspects of MI with mature spermatozoa have already been described elsewhere (5,6). Advancement of MI techniques has enabled us to use immature male germ cells (spermatogenic cells) as substitutes for spermatozoa. In contrast to the development of ICSI, the development of MI using spermatogenic cells was largely contributed by experiments with animals, especially mice. We will review the technical aspects of MI using spermatogenic cells and its significance for basic researches and clinical practice.
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Ogura, A., Yanagimachi, R. (1999). Microinsemination Using Spermatogenic Cells in Mammals. In: Hamamah, S., Olivennes, F., Mieusset, R., Frydman, R. (eds) Male Sterility and Motility Disorders. Serono Symposia USA. Springer, New York, NY. https://doi.org/10.1007/978-1-4612-1522-6_17
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DOI: https://doi.org/10.1007/978-1-4612-1522-6_17
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