β-Xylosidase Recovery by Reversed Micelles

Use of Cationic Surfactant
  • Francislene Andrea Hasmann
  • Adalberto PessoaJr.
  • Ines Conceicao Roberto
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


β-Xylosidase, an enzyme produced by Penicillium janthinellum fungus, was prepurified by fractionated precipitation with ethanol and extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis(2-hydroxyethyl) ammonium chloride (BDBAC) cationic surfactant. A 25-1 fractional factorial design was employed to evaluate the influence of the following factors on the enzyme extraction: pH (A), conductivity (B), surfactant concentration (C), cosolvent concentration (D) and temperature (E). A statistical analysis of the results revealed that, of the five variables studied, pH, surfactant concentration, and temperature had significant main effects (p < 0.05) on the recovery of β-xylosidase (Y). However, the interactions between pH and temperature, conductivity and cosolvent concentration, conductivity and temperature, BDBAC concentration and temperature also had significant influences. A first-order model (R 2 = 0.98) expressed by the equation Y = 7.73-5.55A - 0.67B + 3.49C - 0.41D + 5.26E + 2.05AB - 3.26AC + 0.96AD - 7.07AE - 3.93BD - 2.19BE + 0.99CD + 0.78CE was proposed to represent the enzyme recovery as a function of the effects that were really significant. This model predicts a recovery value of about 40%, which is similar to that obtained experimentally (39.5%).

Index Entries

Reversed micelles liquid-liquid extraction β-xylosidase cationic surfactant downstream processing 


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  1. 1.
    Biely, P. (1985), Trends. Biotechnol. 3, 286–290.CrossRefGoogle Scholar
  2. 2.
    Wong, K. K. Y., Tan, L. U. L., and Saddler, J. N. (1988), Microbiol. Rev. 52, 305–317.Google Scholar
  3. 3.
    Palma, M. B., Milagres, A. M. F., Prata, A. M. R., and Mancilha, I. M. (1996), Proc. Biochem. 31, 141–145.CrossRefGoogle Scholar
  4. 4.
    Durán, N., Milagres, A. M. F., Esposito, E., Curotto, E., Aguirre, C., Teixeira, M. F. S., Carvalho, S. M. S., and Fernandes, C. C. (1995), Appl. Biochem. Biotechnol. 53, 155–162.CrossRefGoogle Scholar
  5. 5.
    Cortez, E. V. (1998), Recuperação de enzimas xilanolíticas por precipitação. Lorena: FAENQUIL (Tese de mestrado).Google Scholar
  6. 6.
    Costa, S. A., Pessoa, A., Jr., and Roberto, I. C. (1998), Appl. Biochem. Biotechnol. 70-72, 629–639.CrossRefGoogle Scholar
  7. 7.
    Brandani, V., Di Giacomo, G., and Spera, L. (1994), Proc. Biochem. 29, 363–367.CrossRefGoogle Scholar
  8. 8.
    Krei, G. and Hustedt, H. (1992), Chem. Eng. Sci. 47, 99–111.CrossRefGoogle Scholar
  9. 9.
    Pessoa, A., Jr. and Vitolo, M. (1997), Biotechnol. Techn. 11, 421–422.CrossRefGoogle Scholar
  10. 10.
    Pessoa, A., Jr. and Vitolo, M. (1998), Proc. Biochem. 33(3), 291–297.CrossRefGoogle Scholar
  11. 11.
    Luisi, P. L., Giomini, M., Pileni, M. P., and Robinson, B. H. (1988), Biochim. Biophys. Acta 947, 209–246.CrossRefGoogle Scholar
  12. 12.
    Leser, M. E. and Luisi, P. L. (1990), Chimia 44, 270–282.Google Scholar
  13. 13.
    Box, G. E. P., Hunter, G. W., and Hunter, J. S. (1978), Statistics for experimenters— an introduction to design, data analysis, and model building. New York: John Wiley & Sons.Google Scholar
  14. 14.
    Milagres, A. M. F. (1988), Alguns aspectos da regulação de β-xilanases extracelulares de Penicillium janthinellum. Master of Science Thesis, Federal University of Viçosa, Viçosa, Brazil.Google Scholar
  15. 15.
    Vogel, H. J. (1956), Microb. Genet. Bull. 13, 42–43.Google Scholar
  16. 16.
    Kumar, S. and Ramon, D. (1996), FEMS Microbiol. Lett. 135, 287–293.Google Scholar
  17. 17.
    Sulistyo, J., Kamiyama, Y., and Yasui, T. (1995), J. Ferment. Bioeng. 79(1), 77–82.CrossRefGoogle Scholar
  18. 18.
    Puls, J. and Pountanen, K. (1989), in Enzyme Systems for Lignocellulose Degradation, Coughlan, M. P., ed., London: Elsevier, pp. 151–165.Google Scholar
  19. 19.
    Pires, M. J. and Cabrai, J. M. S. (1993), Biotechnol. Prog. 9, 647–650.CrossRefGoogle Scholar
  20. 20.
    Regalado, C., Asenjo, J. A., and Pyle, D. L. (1996), Enzym. Microb. Technol. 18, 332–339.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Francislene Andrea Hasmann
    • 1
  • Adalberto PessoaJr.
    • 2
  • Ines Conceicao Roberto
    • 1
  1. 1.Department of Biotechnology/FAENQUILLorena/SPBrazil
  2. 2.Department of Biochemical and Pharmaceutical Technology/FCFUniversity of São PauloSão Paulo/SPBrazil

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