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β-Xylosidase Recovery by Reversed Micelles

Use of Cationic Surfactant
  • Francislene Andrea Hasmann
  • Adalberto PessoaJr.
  • Ines Conceicao Roberto
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

β-Xylosidase, an enzyme produced by Penicillium janthinellum fungus, was prepurified by fractionated precipitation with ethanol and extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis(2-hydroxyethyl) ammonium chloride (BDBAC) cationic surfactant. A 25-1 fractional factorial design was employed to evaluate the influence of the following factors on the enzyme extraction: pH (A), conductivity (B), surfactant concentration (C), cosolvent concentration (D) and temperature (E). A statistical analysis of the results revealed that, of the five variables studied, pH, surfactant concentration, and temperature had significant main effects (p < 0.05) on the recovery of β-xylosidase (Y). However, the interactions between pH and temperature, conductivity and cosolvent concentration, conductivity and temperature, BDBAC concentration and temperature also had significant influences. A first-order model (R 2 = 0.98) expressed by the equation Y = 7.73-5.55A - 0.67B + 3.49C - 0.41D + 5.26E + 2.05AB - 3.26AC + 0.96AD - 7.07AE - 3.93BD - 2.19BE + 0.99CD + 0.78CE was proposed to represent the enzyme recovery as a function of the effects that were really significant. This model predicts a recovery value of about 40%, which is similar to that obtained experimentally (39.5%).

Index Entries

Reversed micelles liquid-liquid extraction β-xylosidase cationic surfactant downstream processing 

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Francislene Andrea Hasmann
    • 1
  • Adalberto PessoaJr.
    • 2
  • Ines Conceicao Roberto
    • 1
  1. 1.Department of Biotechnology/FAENQUILLorena/SPBrazil
  2. 2.Department of Biochemical and Pharmaceutical Technology/FCFUniversity of São PauloSão Paulo/SPBrazil

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