Effect of C:N Molar Ratio on Monomer Composition of Polyhydroxyalkanoates Produced by Pseudomonas mendocina 0806 and Pseudomonas pseudoalkaligenus YS1

  • Kui Hong
  • Guo Qiang Chen
  • Peter Hoi Fu Yu
  • Guang Zhang
  • Ye Liu
  • Hong Chua
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable polymers produced by bacteria. In this study, the effect of C:N molar ratio on the monomer composition of PHAs was investigated, including medium chain length PHA produced by Pseudomonas mendocina 0806 and PHA blends consisting of monomers of 3-hydroxybutyrate and medium chain length hydroxy-alkanoate produced by Pseudomonas pseudoalkaligenus YS1. It was observed that there were some fixed ranges of C:N molar ratio that affect the monomer composition of PHA independently of the substrate. For strain 0806, the ranges were C:N & < 20, 20 < C:N < 200, and C:N > 200. The monomer composition was constant among these ranges when using glucose and octanoate as the sole substrate. For strain YS1, the ranges were C:N < 20, 20 < C:N < 45, and C:N > 45. These results are useful for controlling monomer composition in PHA production.

Index Entries

Polyhydroxyalkanoates polyhydroxybutyrate Pseudomonas mendocina Pseudomonas pseudoalkaligenus 

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Kui Hong
    • 1
  • Guo Qiang Chen
    • 2
  • Peter Hoi Fu Yu
    • 3
  • Guang Zhang
    • 2
  • Ye Liu
    • 2
  • Hong Chua
    • 4
  1. 1.Engineering SchoolSouth China University of Tropical AgricultureDanzhou, HainanChina
  2. 2.Department of Biological Sciences and BiotechnologyTsinghua UniversityBeijingChina
  3. 3.Department of Applied Biology and Chemical TechnologyHong Kong Polytechnic UniversityHung Hom, Hong KongChina
  4. 4.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityHung Hom, Hong KongChina

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