Influence of Lignocellulose-Derived Aromatic Compounds on Oxygen-Limited Growth and Ethanolic Fermentation by Saccharomyces cerevisiae

  • Simona Larsson
  • Alexis Quintana-Sáinz
  • Anders Reimann
  • Nils-Olof Nilvebrant
  • Leif J. Jönsson
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Phenolic compounds released and generated during hydrolysis inhibit fermentation of lignocellulose hydrolysates to ethanol by Saccharomyces cerevisiae. A wide variety of aromatic compounds form from lignin, which is partially degraded during acid hydrolysis of the lignocellulosic raw material. Aromatic compounds may also form as a result of sugar degradation and are present in wood as extractives. The influence of hydroxy-methoxy-benzaldehydes, diphenols/quinones, and phenylpropane derivatives on S. cerevisiae cell growth and ethanol formation was assayed using a defined medium and oxygen-limited conditions. The inhibition effected by the hydroxy-methoxybenzaldehydes was highly dependent on the positions of the substituents. A major difference in inhibition by the oxidized and reduced form of a diphenol/quinone was observed, the oxidized form being the more inhibitory. The phenylpropane derivatives were examined with respect to difference in toxicity depending on the oxidation-reduction state of the γ-carbon, the presence and position of unsaturated bonds in the aliphatic side chain, and the number and identity of hydroxyl and methoxyl substituents. Transformations of aromatic compounds occurring during the fermentation included aldehyde reduction, quinone reduction, and double bond saturation. Aromatic alcohols were detected as products of reductions of the corresponding aldehydes, namely hydroxy-methoxy-benzaldehydes and coniferyl aldehyde. High molecular mass compounds and the corresponding diphenol were detected as products of quinone reduction. Together with coniferyl alcohol, dihydroconiferyl alcohol was identified as a major transformation product of coniferyl aldehyde.

Index Entries

Aromatic compounds inhibitors S. Cerevisiae ethanolic fermentation growth 

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Simona Larsson
    • 1
  • Alexis Quintana-Sáinz
    • 1
  • Anders Reimann
    • 2
  • Nils-Olof Nilvebrant
    • 2
  • Leif J. Jönsson
    • 1
  1. 1.Department of Applied MicrobiologyLund University/Lund Institute of TechnologyLundSweden
  2. 2.STFISwedish Pulp and Paper Research InstituteStockholmSweden

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