Abstract
Construction and comparison of recombinant Escherichia coli strains harboring the polyhydroxybutyrate (PHB) operon from Ralstonia eutropha using vectors possessing different promotors, as well as the production of PHB from soy waste by the recombinant strain, are reported. The lac promotor was the most efficient on expression of the phb operon among the three promotors studied: i.e., lac promotor, T7 promotor and the normal σ70 promotor. The pKS/PHB was the most efficient plasmid for phb operon expression among the three plasmids used: i.e., pKS-, pAED4, and pJM9131. It was observed that isopropyl-β-D-thiogalactopyranoside was not required for the induction of the expression of phb operon. The cell dry wt and polyhydroxyalkanoate content by E. coli XL-1 Blue (pKS/PHB) were 3.025 g/L and 27.83%, respectively.
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© 2000 Springer Science+Business Media New York
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Hong, K. et al. (2000). Construction of Recombinant Escherichia coli Strains for Polyhydroxybutyrate Production Using Soy Waste as Nutrient. In: Finkelstein, M., Davison, B.H. (eds) Twenty-First Symposium on Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-1392-5_29
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DOI: https://doi.org/10.1007/978-1-4612-1392-5_29
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-7128-4
Online ISBN: 978-1-4612-1392-5
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