In Situ Hybridization Using the bDNA Technology

  • Vincent P. Antao
  • Audrey N. Player
  • Janice A. Kolberg


The branched DNA (bDNA) technology has been used quite extensively in the microwell format to determine the levels of viral nucleic acids, as well as specific cellular RNAs [1, 2]. The bDNA technology, which is based on signal amplification, is easy to use, and does not require the use of radioactivity. With the microwell format, it has been shown to be sensitive and specific. The technology offers the additional benefits of a wide dynamic range, as well as accurate and precise quantification. We have recently applied the bDNA technology to the in situ detection of nucleic acids. In situ hybridization allows for the identification of individual cells that have a higher (or lower) level of a particular nucleic acid sequence, in the background of many cells that have normal levels. In addition, in situ hybridization also gives the morphological context of these “positive” cells, which in many applications is very important.


Target Probe Moist Chamber Viral Nucleic Acid Normal Human Bronchial Epithelial Nucleic Acid Target 
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© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Vincent P. Antao
  • Audrey N. Player
  • Janice A. Kolberg

There are no affiliations available

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