µ-Opioid Receptor

Purification and Reconstitution with GTP-Binding Protein
  • Hiroshi Ueda
  • Masamichi Satoh
Part of the Receptor Purification book series (RP, volume 1)

Abstract

It was reported in the 1960s that opioids show various actions through specific opioid receptors in in vitro studies (Kosterlitz and Watt, 1968). In 1971 and 1973, opiate receptors were reported by several independent investigators, in a ligand binding study in brain membranes (Goldstein et al., 1971; Pert and Snyder, 1973; Simon et a1.,1973; Terenius, 1973). The study of opioid receptors was accelerated by the discovery of endogenous opioids, such as enkephalins (Hughes et al., 1975), β-endorphin, dynorphin, and their related peptides (Goldstein, 1984; Yamashiro and Li, 1984). Biochemical, pharmacological, and behavioral studies provided the evidence that various actions of opiates and opioid peptides in the brain and peripheral tissues are mediated by interactions with different subtypes of opioid receptors, such as µ-, δ-, and κ-opioid receptors (Martin et al., 1976; Lord et al., 1977). Furthermore, the discovery of selective ligands for each subtype of opioid receptor (Paterson et al., 1984) made it easy to study the receptor subtype-specific responses in vivo and in vitro. For instance, in in vivo studies, the µ-opioid receptor is thought to be closely relevant to the production of analgesia in the brain (Satoh et al., 1983; Porreca et al., 1984). On the other hand, there are accumulating findings in vitro that µ- and δ- receptors are functionally coupled to the stimulation of potassium ion channel activity (North et al., 1987), whereas κ-receptor indicates an inhi-bition of calcium ion channel activity (Gross and Macdonald, 1987). In addition, µ-and δ-receptors are functionally coupled to the inhibition of adenylate cyclase in membranes of the brain and NG108-15 cells (Collier and Roy, 1974; Klee and Nirenberg, 1976).

Keywords

Morphine Pyridine Nitrite Sarcoma Guanine 

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References

  1. Burgoyne, R. D. (1983) J. Neurochem. 40, 324–331.PubMedCrossRefGoogle Scholar
  2. Burns, D., Hewlett, E. L., Moss, J., and Vaughan, M. (1983) J. Biol. Chem. 258,1435–1438.PubMedGoogle Scholar
  3. Cerione, R. A., Regan, J. W., Nakata, H., Codina, J., Benovic, J. L., Gierschik, P., Somers,R., Spiegel, A. M., Birnbaumer, L., and Lefkowitz, R. J. (1986) J. Biol. Chem. 261,3901–3909.PubMedGoogle Scholar
  4. Cho, T. M., Hasegawa, J., Ge, B., and Loh, H. H. (1986) Proc. Natl. Acad. Sci. USA 83,4138–4142.PubMedCrossRefGoogle Scholar
  5. Collier, H. O. J. and Roy, A. C. (1974) Nature (London) 248, 24–27.CrossRefGoogle Scholar
  6. Fujioka, T., Inoue, F., and Kuriyama, M. (1985) Biochem. Biophys. Res. Comm. 131,640–646.PubMedCrossRefGoogle Scholar
  7. Gilman, A. G. (1987) Ann. Rev. Biochem. 56, 615–649.PubMedCrossRefGoogle Scholar
  8. Gioannini, T. L., Howard, A. D., Hiller, J. M., and Simon, E. J. (1985) J. Biol. Chem. 260, 15117–15121.PubMedGoogle Scholar
  9. Goldstein, A. (1984) in The Peptides, vol. 6 (Udenfriend, S. and Meienhofer, J., eds.), Academic, Orlando, FL, pp. 95–145.Google Scholar
  10. Goldstein, A., Lowney, L. I., and Pal, B. K. (1971) Proc. Natl. Acad. Sci. USA 68,1742–1747.PubMedCrossRefGoogle Scholar
  11. Gross, R. A. and Macdonald, R. L. (1987) Proc. Natl. Acad. Sci. USA 84,5469–5473.PubMedCrossRefGoogle Scholar
  12. Hara, M., Tamaoki, T., and Nakamura, H. (1988) Oncogene Res. 2,325–333.PubMedGoogle Scholar
  13. Harada, M., Ueda, M., Wada, Y., Katada, T., Ui, M., and Satoh, M. (1989) Neurosci. Lett. 100,221–226.PubMedCrossRefGoogle Scholar
  14. Ho, A. K. S., Ling, Q.-L., Duffield, R., Lam, P. H., and Wang, J. H. (1987) Biochem. Biophys. Res. Comm. 142,911–918.PubMedCrossRefGoogle Scholar
  15. Hughes, J., Smith, T. W., Kosterlitz, H. W., Fothergill, L. A., Morgan, B. A., and Morris, H. R. (1975) Nature (London) 258, 577–579.CrossRefGoogle Scholar
  16. Itoh, H., Toyama, R., Kozasa, T., Tsukamoto, T., Matsuoka, M., and Kaziro, Y. (1988)J. Biol. Chem. 263,6656–6664.PubMedGoogle Scholar
  17. Katada, T., Oinuma, M., Kusakabe, K., and Ui, M. (1987) FEBS Lett. 213, 353–358.PubMedCrossRefGoogle Scholar
  18. Katada, T., Oinuma, M., and Ui, M. (1986) J. Biol. Chem. 261, 8182–8191.PubMedGoogle Scholar
  19. Kelleher, D. J., Pessin, J. E., Ruoho, A. E., and Johnson, G. L. (1984) Proc. Natl. Acad. Sci. USA 81, 4316–4320.PubMedCrossRefGoogle Scholar
  20. Klee, W. A. and Nirenberg, M. (1976) Nature (London) 163,609–612.CrossRefGoogle Scholar
  21. Kosterlitz, H. W. and Watt, A. J. (1968) Br. J. Pharmacol. 33,266–276.Google Scholar
  22. Kurose, H., Katada, T., Amano, T., and Ui, M. (1983) J. Biol. Chem. 258,4870–4875.PubMedGoogle Scholar
  23. Kurose, H., Katada, T., Haga, T., Haga, K., Ichiyama, A., and Ui, M. (1986) J. Biol. Chem. 261, 6423–6428.PubMedGoogle Scholar
  24. Kurose, H. and Ui, M. (1985) Arch. Biochem. Biophys. 238,424–434.PubMedCrossRefGoogle Scholar
  25. Lord, J. A. H., Waterfield, A. A., Hughes, J., and Kosterlitz, H. W. (1977) Nature (London) 267, 495–499.CrossRefGoogle Scholar
  26. Lowney, L. I., Schulz, K., Lowery, P., and Goldstein, A. (1974) Science 183, 749–753.PubMedCrossRefGoogle Scholar
  27. Maneckjee, R., Zukin, R. S., Archer, S., Michael, J., and Osei-Gyimah, P. (1985) Proc. Natl. Acad. Sci. USA 82,594–598.PubMedCrossRefGoogle Scholar
  28. Martin, W. R., Eades, C. G., Thompson, J. A., Huppher, R. E., and Gilbert, P. E. (1976) J. Pharmacol. Exp. Then. 197, 517–532.Google Scholar
  29. Matozaki, T., Sakamoto, C., Nagao, M., and Baba, S. (1986) J. Biol. Chem. 261,1414–1420.PubMedGoogle Scholar
  30. Neer, E. J. and Clapham, D. E. (1988) Nature (London) 333,129–134.CrossRefGoogle Scholar
  31. North, A., Williams, J. T., Surprenant, A., and Christie, M. J. (1987) Proc. Natl. Acad. Sci. USA 84, 5487–5491.PubMedCrossRefGoogle Scholar
  32. Paterson, S. J., Robson, L. E., and Kosterlitz, H. W. (1984) in The Peptides, vol. 6 (Udenfriend, S. and Meienhofer, J., eds.), Academic, Orlando, FL, pp. 147–189.Google Scholar
  33. Pedersen, S. E. and Ross, E. M. (1982) Proc. Natl. Acad. Sci. 79, 7228–7232.PubMedCrossRefGoogle Scholar
  34. Pert, C. B. and Snyder, S. H. (1973) Science 179,1011–1014.PubMedCrossRefGoogle Scholar
  35. Porreca, F., Mosberg, H. I., Hurst, R., Hruby, V. J., and Burks, T. F. (1984) J. Pharmacol. Exp. Ther. 230, 341–348.PubMedGoogle Scholar
  36. Satoh, M., Kubota, A., Iwama, T., Yasui, M., Fujibayashi, K., and Takagi, H. (1983) Life Sci. 33 (suppl. I), 689–692.PubMedCrossRefGoogle Scholar
  37. Sibley, D. R. and Lefkowitz, R. J. (1985) Nature (London) 317,124–129.CrossRefGoogle Scholar
  38. Simon, E. J., Hiller, J. M., and Edelman, I. (1973) Proc. Natl. Acad. Sci. USA 70,1947–1949.PubMedCrossRefGoogle Scholar
  39. Simon, E. J., Hiller, J. M., and Edelman, I. (1975) Science 190, 389–390.PubMedCrossRefGoogle Scholar
  40. Simonds, W. F., Burke, T. R., Rice, K. C., Jacobson, A. E., and Klee, W. A. (1985) Proc. Natl. Acad. Sci. USA 82,4974–4978.PubMedCrossRefGoogle Scholar
  41. Terenius, L. (1973) Acta Pharmacol. Toxicol. 33,377–384.CrossRefGoogle Scholar
  42. Ueda, H., Harada, H., Nozaki, M., Katada, T., Ui, M., Satoh, M., and Takagi, H. (1988a) Proc. Natl. Acad. Sci. USA 85, 7013–7017.CrossRefGoogle Scholar
  43. Ueda, H., Harada, H., Misawa, H., Nozaki, M., and Takagi, H. (1987a) Neurosci. Lett. 75, 339–344.CrossRefGoogle Scholar
  44. Ueda, H., Harada, H., Wada, Y., Katada, T., Ui, M., Takagi, H., and Satoh, M. (1988b) Abstracts of 18th Annual Meeting of Society for Neuroscience,Toronto, p. 1053.Google Scholar
  45. Ueda, H., Misawa, H., Fukushima, N., and Takagi, H. (1987b) Eur. J. Pharmacol. 138, 129–132.CrossRefGoogle Scholar
  46. Ueda, H., Misawa, H., Katada, T., Ui, M., Takagi, H., and Satoh, M. (1989) in Advances in the Biosciences (Cros, J., Meunier, J.-C., and Hamon, M., eds.), Pergamon Press, Great Britain, pp. 129–132.Google Scholar
  47. Ueda, H., Misawa, H., Katada, T., Ui, M., Takagi, H., and Satoh, M. (1990) J. Neurochem., in press.Google Scholar
  48. Ueda, H. and Satoh, M. (1988) in Advances in Endocrinology (Imura, H., ed.), Elsevier, Netherlands, pp. 1137–1142.Google Scholar
  49. Ui, M. (1984) Trends Pharmacol. Sci. 5, 277–279.CrossRefGoogle Scholar
  50. Yamashiro, D. and Li, C. H. (1984) in The Peptides, vol. 6 (Udenfriend, S. and Meienhofer, J., eds.), Academic, Orlando, FL pp. 191–217.Google Scholar

Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Hiroshi Ueda
  • Masamichi Satoh

There are no affiliations available

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