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Purification of β,γ Subunits of Guanine Nucleotide Binding Proteins

  • Deirdre Cooney
  • Alan K. Keenan
Part of the Receptor Purification book series (RP, volume 1)

Abstract

Guanine nucleotide binding proteins (G proteins) are now known to constitute a family of regulatory or transducer molecules that link external membrane-bound receptors to corresponding intracellular effector signals in a wide variety of cell types. These established/putative effector systems are summarized in Table 1 for G proteins that have been purified to homogeneity and whose functions have been directly tested to date. It is important to realize that the fact that a particular purified G protein can be shown to stimulate a given effector in a reconstitution system does not prove this to be the function normally served by that G protein in situ.

Table 1

Functions of Purified G Proteins

G protein

Effector system

Reference

Gs

a) Adenylate cyclase catalytic unit

b) Cardiac calcium channels

Codina et al.,1984a,b; Strittmatter and Neer, 1980; May et al., 1985; Neer et al., 1987

Yatani et al., 1987

Gi

a) Adenylate cyclase catalytic unit

b) f-MLP-stimulated PI turnover

Hildebrandt et a1.,1984

Kikuchi et al., 1986

Go

a) f-MLP-stimulated PI turnover

b) Neuronal calcium channels

Hescheler et al., 1987

Kikuchi et al., 1986

GT

a) Retinal cGMP phospho-diesterase

b) Gs-stimulated adenylate-cyclase catalytic unit

Fung et a1., 1981

Cerione et al., 1985

Keywords

Adenylate Cyclase Pertussis Toxin Rabbit Liver Guanine Nucleotide Binding Protein Inhibit Adenylate Cyclase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Deirdre Cooney
  • Alan K. Keenan

There are no affiliations available

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