Purification of the Human Interferon-γ Receptor by Ligand Affinity
Interferon-γ(IFN-γ) is a product of activated T lymphocytes (Kasahara et al., 1983) and low density lymphocytes (Fischer and Rubinstein, 1983). In addition to its antiviral and growth inhibitory activities, IFN-γis one of the major immunoregulatory lymphokines. Its immunoregulatory functions include macrophage activation, growth, differentiation, and maturation of various immunocytes and induction of class I and II MHC gene products both in macrophages and in cells of nonhematopoietic origin (for a review see Trinchieri and Perrusia, 1985). A survey of its various activities reveals that IFN-γelicits 50% of its maximal effect at concentrations of 0.5-1 pM. Indeed, the level of IFN-γ in blood or in other tissue fluids rarely exceeds the lower limit of detection, which is about 10 antiviral U/mL (10 pM). Therefore, a highly sensitive and selective system must be present in the cells in order to trace IFN-γ and respond to it. Such a system is the cell surface receptor. In this respect, IFN-γ is similar to other polypeptide hormones, lymphokines, and other cytokines, all acting through specific cell-membrane receptors. This array of polypeptides and their receptors serve as an efficient chemical signaling network that is essential for the existence of multicellular organisms.
KeywordsHeLa Cell Ascitic Fluid Cold Phosphate Buffer Saline Immunoaffinity Chromatography Nitrocellulose Sheet
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