Insulin Receptor Isolation Through Avidin-Biotin Technology
The ability to quantitate hormone receptors through binding of their radiolabeled ligands has significantly enlarged our view of endocrine disorders. For example, the finding that the plasma membrane concentration of receptors is a dynamic property of the cell, i.e., that receptor number can decrease in the presence of chronically elevated concentrations of hormones and, thus, dampen the cellular response to the hormone, has given us a new insight into the basis for endocrine dysfunction. A better understanding of the receptors themselves should help us to identify abnormal receptors and lead to an appreciation of the function of the hormone-receptor complex.
KeywordsInsulin Receptor Wheat Germ Agglutinin Insulin Binding Affinity Resin Receptor Preparation
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