Abstract
Most human cancers appear to be clonal in origin; that is, they are derived from a single cell that has apparently undergone the process of malignant transformation in vivo. Whether the progeny of this cell will eventually give rise to an invasive, malignant tumor depends upon a number of host and tissue factors. The malignant transformation of cells in vitro is also a complex, multi-step process by which normal cells acquire the various phenotypic characteristics of cancer cells. Three major steps appear to be involved: the development of morphologic transformation, immortality, and tumorigenicity. Although rodent cells will readily undergo immortalization in vitro either spontaneously or in response to treatment with chemical or physical carcinogens, immortalization appears to be the rate-limiting step in the transformation of human diploid cells (1).
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© 1991 Springer Science+Business Media New York
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Little, J.B., Su, LN., Kano, Y. (1991). Transformation of Human Diploid Fibroblasts by Radiation and Oncogenes. In: Rhim, J.S., Dritschilo, A. (eds) Neoplastic Transformation in Human Cell Culture. Experimental Biology and Medicine, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0411-4_7
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DOI: https://doi.org/10.1007/978-1-4612-0411-4_7
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-6750-8
Online ISBN: 978-1-4612-0411-4
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