Skip to main content
SpringerLink
Log in

Transformation in vitro of Human Uroepithelial Cells

  • Chapter
Neoplastic Transformation in Human Cell Culture

Part of the book series: Experimental Biology and Medicine ((EBAM,volume 25))

  • 71 Accesses

Abstract

Normal human uroepithelial cells can now be routinely cultured in vitro, immortalized by SV40 T antigen oncoprotein gene, and tumorigenically transformed after exposure to oncogenic agents including the human bladder carcinogen, 4-aminobiphenyl (ABP) and its metabolites and mutant EJ/ras to carcinoma phenotypes that resemble human bladder cancers. Neoplastic transformation of HUC in vitro is accompanied by chromosome changes that recapitulate many cytogenetic changes reported in clinical bladder cancers.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. E. Silverberg, J.A. Lubera. Cancer 38, 5 (1988).

    CAS  Google Scholar 

  2. W.F. Whitmore, Jr. Urol. (Suppl.) 21, 5 (1988).

    Google Scholar 

  3. F.K. Mostofi, C.J. Davis, Jr., I.A. Sesterhenn. In: Advances in Urologic Oncology, pp. 1–20 (1988).

    Google Scholar 

  4. D. Raghavan, W.U. Shipley, M.B. Garnick, P.J. Russell, J.P. Richie. New Engl. J. Med. 322 (1990).

    Google Scholar 

  5. P.A. Schulte, K. Ringen, et al. J. Occupational Med. 27, 115 (1985).

    CAS  Google Scholar 

  6. S.H. Moolgavkar, R.G. Stevens. J. Natl. Cancer Inst. 67, 15 (1981).

    PubMed  CAS  Google Scholar 

  7. E.J. Zingg, D.M.A. Wallace. Bladder Cancer, Springer-Verlag (1985).

    Book  Google Scholar 

  8. P. Vineis, N. Caproraso, et al. Cancer Res. 50, 3002 (1990).

    PubMed  CAS  Google Scholar 

  9. T. Enomoto, J.M. Ward, A.O. Perantoni. Carcinogenesis 11, 2233 (1990).

    Article  PubMed  CAS  Google Scholar 

  10. V.R. Babu, B.J. Miles, et al. Third International Workshop on Chromosomes in Solid Tumors, Abstract #27, 29 (1989).

    Google Scholar 

  11. V.R. Babu, M.D. Lutz, et al. Cancer Res. 47, 6800 (1987).

    PubMed  CAS  Google Scholar 

  12. A.A. Sandberg, C. Turc-Carel, R.M. Gemmill. Cancer Res. 48, 1049 (1988).

    PubMed  CAS  Google Scholar 

  13. Y.C. Tsai, P.W. Nichols, et al. Cancer Res. 50, 44 (1990).

    PubMed  CAS  Google Scholar 

  14. A.F. Olumi, Y.C. Tsai, et al. Cancer Res. 50, 7081 (1990).

    PubMed  CAS  Google Scholar 

  15. E.R. Fearon, A.P. Feinberg, et al. Nature 318, 377 (1985).

    Article  PubMed  CAS  Google Scholar 

  16. D. Sidransky, A. Von Eschenbach, et al. Science 252, 706 (1991).

    Article  PubMed  CAS  Google Scholar 

  17. J.M. Horowitz, S-H. Park, et al. Proc. Natl. Acad. Sci. 87, 2775 (1990).

    Article  PubMed  CAS  Google Scholar 

  18. J. Presti, T. Galan et al. Proc. Amer. Assoc. Cancer Res. 32, 308 (1991).

    Google Scholar 

  19. E.R. Fearon, B. Vogelstein. Cell 61, 759 (1990).

    Article  PubMed  CAS  Google Scholar 

  20. C.A. Reznikoff, M.D. Johnson, D.H. Norback, G.T. Bryan. In Vitro 19, 326 (1983).

    Article  PubMed  CAS  Google Scholar 

  21. W.W. Schmidt, E.M. Messing, C.A. Reznikoff. J. Urol. 132, 1262 (1984).

    PubMed  CAS  Google Scholar 

  22. C.A. Reznikoff, L.J. Loretz, et al. J. Cell. Physiol. 131, 285 (1987).

    Article  PubMed  CAS  Google Scholar 

  23. L.J. Loretz, C.A. Reznikoff. In Vitro Cell. & Develop. Biol. 24, 333 (1988).

    Article  CAS  Google Scholar 

  24. C.A. Reznikoff, L.J. Loretz, M.D. Johnson, S. Swaminathan. Carcinogenesis 7, 1625 (1986).

    Article  PubMed  CAS  Google Scholar 

  25. B.J. Christian, L.J. Loretz, T.D. Oberley, C.A. Reznikoff. Cancer Res. 47, 6066 (1987).

    PubMed  CAS  Google Scholar 

  26. C. Kao, S-Q. Wu, M. Bhatthacharya, L.F. Meisner, C.A. Reznikoff. Submitted.

    Google Scholar 

  27. L.F. Meisner, S-Q. Wu, B.J. Christian, C.A. Reznikoff. Cancer Res. 48, 3215 (1988).

    PubMed  CAS  Google Scholar 

  28. C.A. Reznikoff, L.J. Loretz, B.J. Christian, S-Q. Wu, L.F. Meisner. Carcinogenesis 9, 1427 (1988).

    Article  PubMed  CAS  Google Scholar 

  29. E.A. Bookland, S. Swaminathan, K.W. Gilchrist, R. Oyasu, C.A. Reznikoff. Submitted

    Google Scholar 

  30. S-Q. Wu, B.E. Storer, E.A. Bookland, A.J. Klingelhutz, K.W. Gilchrist, L.F. Meisner, R. Oyasu, C.A. Reznikoff. Cancer Res., in press.

    Google Scholar 

  31. A.J. Klingelhultz, E.A. Bookland, Genes Chromosomes and Cancer, in press.

    Google Scholar 

  32. A.J. Klingelhultz, S-Q. Wu, C.A. Reznikoff. submitted.

    Google Scholar 

  33. B.J. Christian, C. Kao, S-Q. Wu, L.F. Meisner, C.A. Reznikoff. Cancer Res. 50, 4779 (1990).

    PubMed  CAS  Google Scholar 

  34. C.I. Pratt, R. Oyasu, C.A. Reznikoff. Submitted.

    Google Scholar 

  35. C.I. Pratt, M. Bhattacharya, S-Q. Wu, C.A. Reznikoff. Submitted.

    Google Scholar 

  36. D. Theodorescu, I. Cornil, et al. Proc. Natl. Acad. Sci. USA 87, 9047 (1990).

    Article  PubMed  CAS  Google Scholar 

  37. T.E. Ahlering, L. Dubeau, P.A. Jones. Cancer Res. 47, 6660 (1987).

    PubMed  CAS  Google Scholar 

  38. A.J. Levine, J. Momand. Biochim. et Biophys. Acta 1032, 119 (1990).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Oncology and Clinical Cancer Center, University of Wisconsin, Madison, Wisconsin, 53792, USA

    C. A. Reznikoff, C. Kao, E. A. Bookland, A. J. Klingelhutz, C. I. Pratt, S. Q. Wu, K. W. Gilchrist & S. Swaminathan

Authors
  1. C. A. Reznikoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Kao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Bookland
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. J. Klingelhutz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. I. Pratt
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. Q. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. W. Gilchrist
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. S. Swaminathan
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD, USA

    Johng S. Rhim

  2. Department of Radiation Medicine, Georgetown University Medical Center, Washington DC, USA

    Anatoly Dritschilo

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media New York

About this chapter

Cite this chapter

Reznikoff, C.A. et al. (1991). Transformation in vitro of Human Uroepithelial Cells. In: Rhim, J.S., Dritschilo, A. (eds) Neoplastic Transformation in Human Cell Culture. Experimental Biology and Medicine, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0411-4_27

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-0411-4_27

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6750-8

  • Online ISBN: 978-1-4612-0411-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation