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Biochemical Methods and Assays

  • Paul L M. van Giersbergen
  • Stephen H. Buck
Part of the The Receptors book series (REC)

Abstract

The tachykinins are a family of peptides that share the common C-terminal sequence These peptides exert their biological effects via three pharmacologically and molecular biologically well-defined receptors (see other chapters in this volume), the receptors. Substance P (SP) binds preferentially to the receptor, neurokinin A (NKA) to the receptor, and neurokinin B (NKB) to the receptor. Among other things, the number of receptor sites in tissues/cells and the affinities of compounds/peptides can be measured readily in receptor binding assays that exist for each individual tachykinin receptor. In this chapter, we will describe the development of these assays and the various factors that can influence their outcome. For reasons of clarity, the current tachykinin receptor nomenclature has been used throughout the chapter.

Keywords

Divalent Cation Guanine Nucleotide Incubation Buffer Tachykinin Receptor Radioligand Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Paul L M. van Giersbergen
  • Stephen H. Buck

There are no affiliations available

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