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RT-PCR and Gene Expression

  • Didier Montarras
  • Christian Pinset
  • Jamel Chelly
  • Axel Kahn

Abstract

The application of the polymerase chain reaction technique (PCR) to the study of gene expression, variously referred in the literature to as cDNA-PCR reverse transcription-PCR (RT-PCR) (Chelly et al., 1988; Rappolee et al., 1988a) and sometimes as Patty (PCR aided transcript titration assay, Becker-Andre and Hahlbrock, 1989), represents a dramatic technical innovation. The RT-PCR procedure has proven more sensitive and discriminating than Northern blot analysis, nuclease protection assay, and in situ hybridization. It is rapid and easy to handle, allows simultaneous analysis of several transcripts from total RNA, and can be used for relative or absolute quantification of mRNAs (Chelly et al., 1990a; Rappolee et al., 1989; Becker-Andre and Hahlbrock, 1989; Wang et al., 1989; Singer-Sam et al., 1990; Gilliland et al., 1990). This technique is very powerful in detecting transcripts that have a low copy number because of their short half-life or low rate of transcription and in detecting transcripts from a small number of cells (even from a single cell) or a small amount of tissue (even from a tissue section).

Keywords

Atrial Natriuretic Factor Follicular Dendritic Cell Dystrophin Gene Preimplantation Mouse Embryo Becker Muscular Dystrophy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bar S, Barnea E, Levy Z, Neuman S, Yaffe D, Nudel U (1990): A novel product of the Duchenne muscular dystrophy gene which greatly differs from the known isoforms in its structure and tissue distribution. Biochem J 272:557–560.PubMedGoogle Scholar
  2. Barnea E, Zuk D, Simantov R, Nudel U, Yaffe D (1990): Specificity of expression of the muscle and brain dystrophin gene promoters in muscle and brain cells. Neuron 5:881–888.PubMedCrossRefGoogle Scholar
  3. Becker-André M, Hahlbrock K (1989): Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY). Nucl Acids Res 17:9437–9446.PubMedCrossRefGoogle Scholar
  4. Berg LP, Wieland K, Millar DS, Schlösser M, Wagner M, Kakkar VV, Reiss J, Cooper DN (1990): Detection of a novel point mutation causing haemophilia A by PCR/direct sequencing of ectopically-transcribed factor VIII mRNA. Hum Genet 85:655–658.PubMedCrossRefGoogle Scholar
  5. Boden SD, Joyce ME, Oliver B, Heydemann A, Bolander ME (1989): Estrogen receptor mRNA expression in callus during fracture healing in the rat. Calcif Tissue Int 45:324–325.PubMedCrossRefGoogle Scholar
  6. Chan D, Cole WG (1991): Low basal transcription of genes for tissue-specific collagens by fibroblasts and lymphoblastoid cells. J Biol Chem 266:12487–12494.PubMedGoogle Scholar
  7. Chelly J, Kaplan JC, Maire P, Gautron S, Kahn A (1988): Transcription of the dystrophin gene in human muscle and non-muscle tissues. Nature (London) 333:858–860.CrossRefGoogle Scholar
  8. Chelly J, Concordet JP, Kaplan JC, Kahn A (1989): Illegitimate transcription: transcription of any gene in any cell type. Proc Natl Acad Sci USA 86: 2617–2621.PubMedCrossRefGoogle Scholar
  9. Chelly J, Montarras D, Pinset C, Berwald-Netter Y, Kaplan JC, Kahn A (1990a): Quantitative estimation of minor mRNAs by cDNA-polymerase chain reaction. Application to dystrophin mRNA in cultured myogenic and brain cells. Eur J Biochem 187:691–658.PubMedCrossRefGoogle Scholar
  10. Chelly J, Hamard G, Koulakoff A, Kaplan JC, Kahn A, Berward-Netter Y (1990b): Dystrophin gene transcribed from different promoters in neuronal and glial cells. Nature (London) 344:64–65.CrossRefGoogle Scholar
  11. Chelly J, Gilgenkrantz H, Lambert M, Hamard G, Chafey P, Recan D, Katz P, de la Chapelle A, Koenig M, Ginjaar IB, Fardeau M, Tome F, Kahn A, Kaplan JC (1990c): Effect of dystrophin gene deletions on mRNA levels and processing in Duchenne and Becker muscular dystrophies. Cell 63:1239–1248.PubMedCrossRefGoogle Scholar
  12. Chelly J, Hugnot JP, Concordet JP, Kaplan JC, Kahn A (1991a): Illegitimate (or ectopic) transcription proceeds through the usual promoters. Biochem Biophys Res Commun 178:553–557.PubMedCrossRefGoogle Scholar
  13. Chelly J, Gilgenkrantz H, Hugnot JP, Hamard G, Lambert M, Recan D, Akli S, Cometto M, Kahn A, Kaplan JC (1991b): Illegitimate transcription: Application to the analysis of truncated transcripts of the dystrophin gene in non-muscle cultured cells from Duchenne and Becker patients. J Clin Invest 88:1161–1197.PubMedCrossRefGoogle Scholar
  14. Chen J, Jones P (1989): Determination genes. Curr Opinion Cell Biol 1:1075–1080.PubMedCrossRefGoogle Scholar
  15. Daubas P, Devillers-Thiery A, Geoffroy B, Martinez S, Bessis A, Changeux JP (1990): Differential expression of the neuronal acetylcholine receptor α2 subunit gene during chick brain development. Neuron 5:49–60.PubMedCrossRefGoogle Scholar
  16. de la Monte SM, Ozturk M, Wands JR (1990): Enhanced expression of an exocrine pancreatic protein in Alzheimer’s disease and the developing human brain. J Clin Invest 86:1004–1013.PubMedCrossRefGoogle Scholar
  17. Dolle P, Castrillo JL, Theill LE, Deerinck T, Ellismaen M, Karin M (1990): Expression of GHF-1 protein in mouse pituitaries correlates both temporally and spatially with the onset of growth hormone gene activity. Cell 60:809–820.PubMedCrossRefGoogle Scholar
  18. Ehlers S, Smith KA (1991): Differentiation of T cell lymphokine gene expression. The in vitro acquisition of T cell memory. J Exp Med 173:25–36.PubMedCrossRefGoogle Scholar
  19. Emerson CP (1990): Myogenesis and developmental control genes. Curr Opinion Cell Biol 2:1065–1075.PubMedCrossRefGoogle Scholar
  20. Feener CA, Koenig M, Kunkel LM (1989): Alternative splicing of human dystrophin mRNA generates isoforms at the carboxy terminus. Nature (London) 338:509–511.CrossRefGoogle Scholar
  21. Fonknechten N, Chelly J, Lepercq J, Kahn A, Kaplan JC, Kitzis A, Chomel JC (1992): CFTR illegitimate transcription in lymphoid cells: Quantification and applications to the investigation of pathological transcripts. Hum Genet 88:508–512.PubMedCrossRefGoogle Scholar
  22. Frohman MA, Dush MK, Martin GR (1988): Rapid production of full-length cDNAs from rare transcripts: Amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85:8998–9002.PubMedCrossRefGoogle Scholar
  23. Gilliland G, Perrin S, Blanchard K, Bunn FF (1990): Analysis of cytokine mRNA and DNA: Detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci USA 87:2725–2729.PubMedCrossRefGoogle Scholar
  24. Golde TE, Estus S, Usiak M, Younkin LH, Younkin SG (1990): Expression of β amyloid protein precursor mRNAs: Recognition of a novel alternatively spliced form and quantitation in Alzheimer’s disease using PCR. Neuron 4:253–267.PubMedCrossRefGoogle Scholar
  25. Haase T, Retzel EF, Staskus KA (1990): Amplification and detection of lentiviral DNA inside cells. Proc Natl Acad Sci USA 87:4971–4975.PubMedCrossRefGoogle Scholar
  26. Haber DA, Buckler AJ, Glaser T, Call KM, Pelletier J, Sohn RL, Douglass EC, Housman DE (1990): An internal deletion within an 11p13 zinc finger gene contributes to the development of Wilms’ tumor. Cell 61:1257–1269.PubMedCrossRefGoogle Scholar
  27. Hansell DJ, Bryant-Greenwood GD, Greenwood FC (1991): Expression of the human relaxin H1 gene in the decidua, trophoblast, and prostaste. J Clin End Met 72:899–904.CrossRefGoogle Scholar
  28. Heyner S, Rao LV, Jarett L, Smith RM (1989): Preimplantation mouse embryos internalize maternal insulin via receptor-mediated encodytosis: Pattern of uptake and functional correlations. Dev Biol 134:48–58.PubMedCrossRefGoogle Scholar
  29. Hugnot JP, Gilgenkrantz H, Vincent N, Chaffey P, Morrist G, Monaco T, Koulakoff A, Berwald-Netter Y, Kaplan JC, Kahn A, Chelly J (1992): Characterization of the products generated by the distal part of the dystrophin gene: mRNAs and protein. Proc Natl Acad Sci USA 89:7506–7510.PubMedCrossRefGoogle Scholar
  30. Iveil R, Furuya K, Brackmann B, Dawood Y, Khan-Dawood F (1990): Expression of the oxytocin and vasopressin genes in human and baboon gonadal tissues. Endocrinology 127:2990–2996.CrossRefGoogle Scholar
  31. Jager RJ, Anvret M, Hall K, Scherer G (1990): A human XY female with a frame shift mutation in the candidate testis-determining gene SRY. Nature (London) 348:452–453.CrossRefGoogle Scholar
  32. Knebelmann B, Boussin L, Guerrier D, Legeai L, Kahn A, Josso N, Picard JY (1991): Anti-Müllerian hormone Bruxelles: A nonsense mutation associated with the persistent Müllerian duct syndrome. Proc Natl Acad Sci USA 88:3767–3371.PubMedCrossRefGoogle Scholar
  33. Koopman P, Münsterberg A, Capel B, Vivian N, Lovell-Badge R (1990): Expression of a candidate sex-determining gene during mouse testis differentiation. Nature (London) 348:450–452.CrossRefGoogle Scholar
  34. Koos RD, Olson CE (1989): Expression of basic fibroblast growth factor in the rat ovary: Detection of mRNA using reverse transcription-poly-merase chain reaction amplification. Mol Endocrinol 3:2041–2048.PubMedCrossRefGoogle Scholar
  35. Leroux A, Junien C, Kaplan JC, Bamberger J (1975): Generalized deficiency of cytochrome b5 reductase in congenital methemoglobinemia with mental retardation. Nature (London) 258:619–620.CrossRefGoogle Scholar
  36. Leroy P, Krust A, Zelent A, Mendolsohn C, Garnier JM, Kastner P, Dierich A, Chambon P (1991): Multiple isoforms of the mouse retinoic acid receptor α are generated by alternative splicing and differential induction by retinoic acid. EMBO J 10:59–69.PubMedGoogle Scholar
  37. Linsk R, Gottesman M, Pernis B (1989): Are tissues a patch quilt of ectopic gene expression? Science 246:261.PubMedCrossRefGoogle Scholar
  38. Loh EY, Elliott JF, Cwirla S, Lanier LL, Davis MM (1989): Polymerase chain reaction with single-sided specificity: Analysis of T cell receptor δ chain. Science 243:217–220.PubMedCrossRefGoogle Scholar
  39. Louache F, Debili N, Cramer E, Breton-Gorius J, Vainchenker W (1991): Fibrinogen is not synthesized by human megakaryocytes. Blood 77:311–316.PubMedGoogle Scholar
  40. Magnuson MA, Shelton KD (1989): An alternate promoter in the glucokinase gene is active in the pancreatic β cell. J Biol Chem 264:15936–15942.PubMedGoogle Scholar
  41. McLeod JF, Cooke NE (1989): The vitamin D-binding protein, α-fetoprotein, albumin multigene family: Detection of transcripts in multiple tissues. J Biol Chem 264:21760–21769.PubMedGoogle Scholar
  42. Mivechi NF, Rossi JJ (1990): Use of polymerase chain reaction to detect the expression of the M r 70,000 heat shock genes in control or heat shock leukemia cells as correlated to their heat response. Cancer Res 50:2877–2884.PubMedGoogle Scholar
  43. Mohler KM, Butler LD (1990): Differential production of IL-2 and IL-4 mRNA in vivo after primary sensitization. J Immunol 145:1734–1739.PubMedGoogle Scholar
  44. Montarras D, Pinset C, Chelly J, Kahn A, Gros F (1989): Expression of MyoD1 coincides with terminal differentiation in determined but inducible muscle cells. EMBO J 8:2203–2207.PubMedGoogle Scholar
  45. Montarras D, Chelly J, Bober E, Arnold H, Ott MO, Gros F, Pinset C (1991): Developmental patterns in the expression of Myf5, MyoD, myogenin, and MRF4 during myogenesis. New Biol 3:592–600.PubMedGoogle Scholar
  46. Moriyama T, Murphy HR, Martin BM, Garcia-Perez A (1990): Detection of specific mRNAs in single nephron segments by use of the polymerase chain reaction. Am J Physiol 258:F. 1470–1474.Google Scholar
  47. Murray R, Lee F, Chiu CP (1990): The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis. Mol Cell Biol 10:4953–4956.PubMedGoogle Scholar
  48. Neve RL, Rogers J, Higgins GA (1990): The Alzheimer amyloid precursor-related transcript lacking the β/A4 sequence is specially increased in Alzheimer’s disease brain. Neuron 5:329–338.PubMedCrossRefGoogle Scholar
  49. Noonan KE, Beck C, Holzmayer TA, Chin JE, Wunder JS, Andrulis IL, Gazdar AF, Willman CL, Griffith B, Von Hoff DD, Roninson IB (1990): Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc Natl Acad Sci USA 87:7160–7164.PubMedCrossRefGoogle Scholar
  50. Nunez DJR, Davenport AP, Brown MJ (1990): Atrial natriuretic factor mRNA and binding sites in the adrenal gland. Biochem J 271:555–558.PubMedGoogle Scholar
  51. Ohara O, Dorit RL, Gilbert W (1989): One-sided polymerase chain reaction: The amplification of cDNA. Proc Natl Acad Sci USA 86:5673–5677.PubMedCrossRefGoogle Scholar
  52. Olson EN (1990): MyoD family: A paradigm for development. Genes Dev 4:1454–1461.PubMedCrossRefGoogle Scholar
  53. Omiecinski CJ, Redlich CA, Costa P (1990): Induction and developmental expression of cytochrome P450IA1 messenger RNA in rat and human tissues: Detection by the polymerase chain reaction. Cancer Res 50:4315–4321.PubMedGoogle Scholar
  54. Ott MO, Bober E, Lyons G, Arnold H, Buckingham M (1991): Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo. Development 111: 1097–1107.PubMedGoogle Scholar
  55. Ozols J, Korza G, Heinemann FS, Hediger MA, Strittmatter P (1985): Complete amino acid sequence of steer liver microsomed NADH-cytochrome b5 reductase. J Biol Chem 260:11953–11961.PubMedGoogle Scholar
  56. Pinset C, Mulle C, Benoit P, Changeux JP, Chelly J, Gros F, Montarras D (1991): Functional adult acetylcholine receptor develops independently of motor innervation in Sol 8 mouse muscle cell line. Embo J 10:2411–2418.PubMedGoogle Scholar
  57. Pizzuti A, Borsani G, Falini A, Rugarli EI, Sidoli A, Baralle FE, Scarlato G, Silani V (1990): Detection of β-nerve growth factor mRNA in the human fetal brain. Brain Res 518:337–341.PubMedCrossRefGoogle Scholar
  58. Poznanski AA, Calarco PG (1991): The expression of intracisternal A particle genes in the preim-plantation mouse embryo. Dev Biol 143:271–281.PubMedCrossRefGoogle Scholar
  59. Rappolee DA, Mark D, Banda MJ, Werb Z (1988a): Wound macrophages express TGF-α and other growth factors in vivo: Analysis by mRNA phenotyping. Science 241:708–712.PubMedCrossRefGoogle Scholar
  60. Rappolee DA, Brenner CA, Schultz R, Mark D, Werb Z (1988b): Developmental expression of PDGF, TGF-α, and TGF-β genes in preimplantation mouse embryos. Science 241:1823–1825.PubMedCrossRefGoogle Scholar
  61. Rappolee DA, Wang A, Mark D, Werb Z (1989): Novel method for studying mRNA phenotypes in single or small numbers of cells. J Cell Biochem 39:1–11.PubMedCrossRefGoogle Scholar
  62. Reyes AA, Small SJ, Akeson R (1991): At least 27 alternatively spliced forms of the neural cell adhesion molecule mRNA are expressed during rat heart development. Mol Cell Biol 11:1654–1661.PubMedGoogle Scholar
  63. Roberts RG, Bentley DR, Barby TFM, Manners E, Bobrow M (1990): Direct diagnostic of carriers of Duchenne and Becker muscular dystrophy by amplification of lymphocyte RNA. Lancet 336: 1523–1526.PubMedCrossRefGoogle Scholar
  64. Roberts RG, Barby TFM, Manners E, Bobrow M, Bentley DR (1991): Direct detection of dystrophin gene rearrangements by analysis of dystrophin mRNA in peripheral blood lymphocytes. Am J Hum Genet 49:298–310.PubMedGoogle Scholar
  65. Rupp RAW, Weintraub H (1991): Ubiquitous MyoD transcription at the midblastula transition precedes induction-dependent MyoD expression in presumptive mesoderm of X. laevis. Cell 65:927–937.PubMedCrossRefGoogle Scholar
  66. Ryan MC, Sandell LJ (1990): Differential expression of a cysteine-rich domain in the amino-terminal propeptide of type II (cartilage) procollagen by alternative splicing of mRNA. J Biol Chem 265:10334–10339.PubMedGoogle Scholar
  67. Salgame P, Abrams JS, Clayberger C, Goldstein H, Convit J, Modlin RL, Bloom BR (1991): Differing lymphokine profiles of functional subsets of human CD4 and CD8 T cell clones. Science 277:279–282.CrossRefGoogle Scholar
  68. Saltini C, Kirby M, Trapnell BC, Tamura N, Crystal RG (1990): Biased accumulation of T lymphocytes with “memory” type CD45 leukocyte common antigen gene expression on the epithelial surface of the human lung. J Exp Med 171:1123–1140.PubMedCrossRefGoogle Scholar
  69. Sandell LJ, Morris N, Robbins JR, Goldring MB (1991): Alternatively spliced type II procollagen mRNAs define distinct populations of cells during vertebral development: Differential expression of the amino-propeptide. J Cell Biol 114: 1307–1319.PubMedCrossRefGoogle Scholar
  70. Sarkar G, Sommer SS (1989): Access to a messenger RNA sequence or its protein product is not limited by tissue or species specificity. Science 244:331–334.PubMedCrossRefGoogle Scholar
  71. Sassoon D, Lyons G, Wright WE, Lin V, Lassar A, Weintraub H, Buckingham M (1989): Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis. Nature (London) 341:303–307.CrossRefGoogle Scholar
  72. Schaefer BC, Woisetschlaeger M, Strominger JL, Speck SH (1991): Exclusive expression of Ep-stein-Barr virus nuclear antigen 1 in Burkitt lymphoma arises from a third promoter, distinct from the promoters used in latently infected lymphocytes. Proc Natl Acad Sci USA 88:6550–6554.PubMedCrossRefGoogle Scholar
  73. Schloesser M, Slomski R, Wagner M, Reiss J, Berg LP, Kakkar VV, Cooper DN (1990): Characterization of pathological dystrophin transcripts from the lymphocytes of a muscular dystrophy carrier. Mol Biol Med 7:519–523.PubMedGoogle Scholar
  74. Schriever F, Freeman G, Nadler LM (1991): Follicular dendritic cells contain a unique gene repertoire demonstrated by single-cell polymerase chain reaction. Blood 77:787–791.PubMedGoogle Scholar
  75. Serrano J, Shuldiner AR, Roberts CT, LeRoith D, de Pablo F (1990): The insulin-like growth factor I (IGF-I) gene is expressed in chick embryos during early organogenesis. Endocrinology 127: 1547–1549.PubMedCrossRefGoogle Scholar
  76. Singer-Sam J, Robinson MO, Bellvé AR, Simon MI, Riggs AD (1990): Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis. Nucl Acids Res 18: 1255–1259.PubMedCrossRefGoogle Scholar
  77. Slomski R, Schloesser M, Chlebowska H, Reiss J, Engel W (1991): Detection of human spermatid-specific transcripts in peripheral blood lymphocytes of males and females. Hum Genet 87:307–310.PubMedCrossRefGoogle Scholar
  78. Smith LR, Kono DH, Theofilopoulos AN (1991): Complexity and sequence identification of 24 rat Vβ genes. J Immunol 147:375–379.PubMedGoogle Scholar
  79. Talian JC, Zelenka PS (1991): Calpactin I in the differentiating embryonic chicken lens: mRNA levels and protein distribution. Dev Biol 143:68–77.PubMedCrossRefGoogle Scholar
  80. Telford NA, Hogan A, Franz CR, Schultz GA (1990): Expression of genes for insulin and insulin-like growth factors and receptors in early postimplantation mouse embryos and embryonal carcinoma cells. Mol Repro Dev 27:81–92.CrossRefGoogle Scholar
  81. Traber PG, Wang W, McDonnell M, Gumucio JJ (1990): P450IIB gene expression in rat small intestine: Cloning of intestinal P450IIB1 mRNA using the polymerase chain reaction and transcriptional regulation of induction. Mol Pharmacol 37:810–819.PubMedGoogle Scholar
  82. Voutilainen R, Ilvesmäki V, Miettinen PJ (1991): Low expression of 3β-hydroxy-5-ene steroid dehydrogenase gene in human fetal adrenals in vivo; adrenocorticotropin and protein kinase C-dependent regulation in adrenocortical cultures. J Clin End Met 72:761–767.CrossRefGoogle Scholar
  83. Wang AM, Doyle MV, Mark DF (1989): Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci USA 86:9717–9721.PubMedCrossRefGoogle Scholar
  84. Weider KJ, Walz G, Zanker B, Sehajpal P, Sharma VK, Skolnik E, Strom TB, Suthanthiran M (1990): Physiologie signaling in normal human T-cells: mRNA phenotyping by Northern blot analysis and reverse transcription-polymerase chain reaction. Cell Immunol 128:41–51. Weizsächer FV, Laheit S, Koch HK, Oehlert W, Gerok W, Blum HE (1991): A simple and rapid method for the detection of RNA in formalin-fixed, paraffin-embedded tissues by PCR amplification. Biochem Biophys Res Commun 174:176–180.Google Scholar
  85. Wesselingh SL, Gough NM, Finlay-Jones J J, McDonald PJ (1990): Detection of cytokine mRNA in astrocyte cultures using the polymerase chain reaction. Lymphokine Res 9:177–185.PubMedGoogle Scholar
  86. Yamamura M, Uyemura K, Deans RJ, Weinberg K, Rea TH, Bloom BR, Moddlin RL (1991): Defining protective responses to pathogens: Cytokine profiles in leprosy lesions. Science 277:277–279.CrossRefGoogle Scholar
  87. Yang F, Friedrichs WE, Cupples RL, Bonifacio MJ, Sanford JA, Horton WA, Bowman BH (1990): Human ceruloplasmin tissue-specific expression of transcripts produced by alternative splicing. J Biol Chem 265:10780–10785.PubMedGoogle Scholar
  88. Yubisui T, Miyata T, Iwanaga S, Tamura M, Yoshida S, Takeshita M, Nakajima H (1984): Amino acid sequence of NADH-cytochrome b5 reductase of human erythrocytes. J Biochem 96:579–582.PubMedGoogle Scholar
  89. Zelent A, Mendelsohn C, Kastner P, Krust A, Garnier JM, Ruffenach F, Leroy P, Chambon P (1991): Differentially expressed isoforms of the mouse retinoic acid receptor β are generated by usage of two promoters and alternative splicing. EMBO J 10:71–81.PubMedGoogle Scholar
  90. Zorn AM, Krieg PA (1991): PCR analysis of alternative splicing pathways: Identification of artifacts generated by heteroduplex formation. Biotechniques 11:181–183.Google Scholar

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© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Didier Montarras
  • Christian Pinset
  • Jamel Chelly
  • Axel Kahn

There are no affiliations available

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