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Application of the Hybridization Protection Assay (HPA) to PCR

  • Norman C. Nelson
  • Sherrol H. McDonough

Abstract

The development of in vitro DNA amplification techniques has made detection of specific sequences more sensitive and rapid than ever before. The ability to amplify rare sequences has greatly improved our ability to detect chromosomal translocations, allelic variability, and infectious agents (Innis et al., 1990). Polymerase chain reaction (PCR) is a method of DNA amplification performed by repeatedly denaturing a DNA target, annealing specific oligonucleotide primers, and extending the primers with a DNA-dependent DNA polymerase (Mullis et al., 1986; Mullis and Faloona, 1987; Saiki et al., 1988b). Each cycle theoretically results in a doubling of the number of target sequences. Other amplification systems, such as the transcription-based amplification system, or TAS (Kwok et al., 1987), also give significant amplification of target sequences.

Keywords

Chlamydia Trachomatis Neisseria Gonorrhoeae Acridinium Ester Unhybridized Probe Differential Hydrolysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Norman C. Nelson
  • Sherrol H. McDonough

There are no affiliations available

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