Application of the Hybridization Protection Assay (HPA) to PCR
The development of in vitro DNA amplification techniques has made detection of specific sequences more sensitive and rapid than ever before. The ability to amplify rare sequences has greatly improved our ability to detect chromosomal translocations, allelic variability, and infectious agents (Innis et al., 1990). Polymerase chain reaction (PCR) is a method of DNA amplification performed by repeatedly denaturing a DNA target, annealing specific oligonucleotide primers, and extending the primers with a DNA-dependent DNA polymerase (Mullis et al., 1986; Mullis and Faloona, 1987; Saiki et al., 1988b). Each cycle theoretically results in a doubling of the number of target sequences. Other amplification systems, such as the transcription-based amplification system, or TAS (Kwok et al., 1987), also give significant amplification of target sequences.
KeywordsChlamydia Trachomatis Neisseria Gonorrhoeae Acridinium Ester Unhybridized Probe Differential Hydrolysis
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