Application of the Hybridization Protection Assay (HPA) to PCR
The development of in vitro DNA amplification techniques has made detection of specific sequences more sensitive and rapid than ever before. The ability to amplify rare sequences has greatly improved our ability to detect chromosomal translocations, allelic variability, and infectious agents (Innis et al., 1990). Polymerase chain reaction (PCR) is a method of DNA amplification performed by repeatedly denaturing a DNA target, annealing specific oligonucleotide primers, and extending the primers with a DNA-dependent DNA polymerase (Mullis et al., 1986; Mullis and Faloona, 1987; Saiki et al., 1988b). Each cycle theoretically results in a doubling of the number of target sequences. Other amplification systems, such as the transcription-based amplification system, or TAS (Kwok et al., 1987), also give significant amplification of target sequences.
KeywordsSurfactant Hydrolysis Lithium Leukemia Amide
Unable to display preview. Download preview PDF.
- Arnold LJ Jr, Bhatt RS, Reynolds MA (1988): Non-nucleotide linking reagents for nucleotide probes. PCT/US88/03173.Google Scholar
- Enns RK (1988): DNA probes: An overview and comparison with current methods. Lab Med 19:295–300.Google Scholar
- Gegg C, Kranig-Brown D, Hussain J, McDonough S, Kohne D, Shaw S (1990): A clinical evaluation of a new DNA probe test for Neisseria gonorrhoeae that included analysis of specimens producing results discrepant with culture. Abstracts of the 90th Annual Meeting of the American Society for Microbiology 356.Google Scholar
- Innis MA, Gelfand DH, Sninsky JJ, White TJ (ed.) (1990): PCR Protocols. San Diego, CA: Academic Press.Google Scholar
- Keller GH, Manak MM (1989): DNA Probes. New York: Stockton Press.Google Scholar
- Kohne DE (1986): Application of DNA probe tests to the diagnosis of infectious disease. Am Clin Prod Rev November:20–29.Google Scholar
- Kranig-Brown D, Gegg C, Hussain J, Johnson R, Shaw S (1990): Use of PCR amplification and unlabeled probe competition for analysis of specimens producing results discrepant with culture in an evaluation of a DNA probe test for Chlamydia trachomatis. Abstracts of the 90th Annual Meeting of the American Society for Microbiology 356.Google Scholar
- Nelson NC, Hammond PW, Wiese WA, Arnold LJ Jr (1990): Homogeneous and heterogeneous chemiluminescent DNA probe-based assay formats for the rapid and sensitive detection of target nucleic acids. In: Luminescence Immunoassay and Molecular Applications. van Dyke K, ed. Boca Raton, FL: CRC Press, 293–309.Google Scholar
- Ou C-Y, McDonough SH, Cabanas D, Ryder TB, Harper M, Moore J, Schochetman G (1990): Rapid and quantitative detection of enzymatically amplified HIV-1 DNA using chemiluminescence-labeled oligonucleotide probes. AIDS Res Human Retrovirus 6:1323–1329.Google Scholar
- Rubin SM, Murphy-Clark KA, Bee GG, Gordon PC, Roberts SS, Johnson R (1990): Development of rapid non-isotopic DNA probe assays for fungi: Blastomyces dermatitidis. Abstracts of the 90th Annual Meeting of the American Society for Microbiology 409.Google Scholar
- Snider E, Gordon P, Dean E, Trainor D (1990): Development of a DNA probe culture confirmation test of Haemophilus influenzae. Abstracts of the 90th Annual Meeting of the American Society for Microbiology 398.Google Scholar
- Watson M, Zepeda-Bakan M, Smith K, Alden M, Stolzenbach F, Johnson R (1990): Evaluation of a DNA probe assay for screening urinary tract infections. Abstracts of the 90th Annual Meeting of the American Society for Microbiology 392.Google Scholar