The Future of DNA Sequencing: Methods and Applications
The Human Genome Project entails the determination of the DNA sequence of a set of samples that will represent a single human genome. The identity of the particular base, A, T, C or G, at each position along 24 pieces of DNA encompassing one example of each of the human (H. sapiens) chromosomes, will be revealed. A total of about 3 billion bases will be identified. Along the way, the DNA sequence of several model genomes will be determined including a worm, C. elegans, a fly, D. melanogaster, a yeast, S. cerevisiae, and a bacterium, E. coli. All of these model genomes together amount to less than 20% of one human genome. In addition, depending on the efficiency and cost of the methods finally employed for most of the sequencing, we will also see revealed some or all of the DNA sequence of the common laboratory mouse, M. musculus. It is clear that, with the technology currently in hand, it will be possible to obtain the set of fragments needed for this DNA sequencing effort (this process is called physical mapping) and then read the sequence of each of these fragments for a total cost approximating the original estimate of $3 billion.
KeywordsHuman Genome Project Model Genome Single Molecule Sequencing Stable Metal Isotope Single Human Genome
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- 4.E. Blake, J. Mihalovich, R. Higuchi, P.S. Walsh and H. Erlich, J. Forens. Sci. 1992, 37, 700–726.Google Scholar
- 5.C. T. Comey, B. Budowle, D. E. Adams, A. L. Baumstark, J. Lindsey and L.A. Presley, J. Forens. Sci. 1993, 38, 239–249.Google Scholar
- 8.G. Wang, R. W. Blakesly, D. E. Berg and C. M. Berg, Proc. Natl. Acad. Sci. USA 1993, 90, 6509–6515.Google Scholar
- 10.H. Voss, S. Wiemann, D. Grothues, D. Sensen, J. Zimmermann, C. Schwager, J. Stegeman, H. Erfle, T. Rupp and W. Ansorge, Biotechniques 1993, 15, 714–721.Google Scholar
- 12.S. Carson, A. S. Cohen, A. Belenkii, M. C. Ruiz-Martinez, J. Berka and B. L. Karger, Anal. Chem. 1993, 56, 3219–3226.Google Scholar
- 13.X. C. Huang, M. A. Quesada and R. A. Mathies, Anal. Chem. 1992, 64, 2149–2154.Google Scholar
- 18.E. M. Southern, U. Maskos and J. K. Elder, Genomics 1992, 13, 1008–1017.Google Scholar
- 20.L. M. Davis, F. R. Fairfield, C. A. Harger, J. H. Jett, R. A. Keller, J. H. Hahn, L. A. Krakowski, B. L. Marrone, J. C. Martin, H. L. Nutter, R. L. Ratliff, E. B. Shera, D. J. Simpson and S. A. Soper, Genet. Anal. Techniq. Applicat. 1991, 8, 1–7.Google Scholar
- 24.M. Reeben and H. Prydz, Biotechniques 1994, 16, 416–417.Google Scholar
- 29.K. J. Wu, T. A. Shaler and C. H. Becker, Anal. Chem. 1993, 66,1627–1645.Google Scholar