Skip to main content

Recovery and Purification of Lactic Acid from Fermentation Broth by Adsorption

  • Chapter
Seventeenth Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: ABAB Symposium ((ABAB,volume 57/58))

Abstract

Riedel-de-Haen VI-15, Dowex MWA-1 and Amberlite IRA-35 were employed for lactic acid recovery using model fermentation broth. The broth was first acidified by using a cation exchanger before sorption of lactic acid onto the basic sor-bents. Lactic acid was completely recovered from the VI-15 column after 7 bed volumes (BV) of methanol, whereas only 64% was recovered from MWA-1 after 4. 5 BV and 18% from IRA-35 after 5 BV. The 5% NH4OH eluted all lactic acid from the MWA-1 column in 1. 5 BV with a maximum effluent concentration of 113 mg/mL. The simple recovery scheme employed was not sufficient to produce heat-stable lactic acid. Other broth components were also adsorbed by the basic sorbents and could not be removed during the rinse step, but eluted with lactic acid during the desorption step.

Author to whom all correspondence and reprint requests should be addressed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. HRA, Inc. (1991) Lactic Acid and Its Derivatives: A Global Review. HRA, Inc., Prairie Village, KS.

    Google Scholar 

  2. Lipinsky, E. S. and Sinclair, R. G. 1986 Chem. Eng. Prog. 82, –32

    CAS  Google Scholar 

  3. Yasuda, S., Niwa, T., and Kurohara, T. (1984), Japanese Patent 59,118–731.

    Google Scholar 

  4. Kiel, K. H., Greiner, U., Engelhardt, F., Kuhlein, K., Heb, G., Reinhold, K., and Schlingmann, M. (1985), German Patent DE 3,32–8093.

    Google Scholar 

  5. Obara, H. (1988), Japanese Patent 63,188–632.

    Google Scholar 

  6. Collin, H. and Buresch, E. (1990), European Patent 03,77–430.

    Google Scholar 

  7. Kulprathipanja, S. and Oroskar, A. R. 1991 US Patent 5,068–418

    Google Scholar 

  8. Rossiter, G. J. (1991), inPreparative and Process-Scale Liquid ChromatographyG. Subramanian, G., ed., Ellis Horwood, West Sussex, UK.

    Google Scholar 

  9. Ernst, E. E. and McQuigg, D. W. (1992), Paper presented at the AIChE Annual National Meeting, November, Miami Beach, FL.

    Google Scholar 

  10. King, C. J. and Tung, L. (1992), US Patent 5,132–456.

    Google Scholar 

  11. Yates, R. A. (1981), US Patent 4,282–323

    Google Scholar 

  12. Srivastava, A., Roychoudhury, P. K., and Sahai, V. (1992) Biotechnol. Bioeng. 39, 607–613

    Article  CAS  Google Scholar 

  13. Tsao, G. T., Lee, S. J., Tsai, G., Seo, J., McQuigg, D. W., Vorhies, S., and Iyer, G. (1993), International Patent Appl. PCT/US92/07738

    Google Scholar 

  14. Vickroy, T. B. (1985), in Comprehensive Biotechnology: The Principles, Applications and Regulations of Biotechnology in Industry, Agriculture and Medicine, Moo-Young, M., ed., Pergamon, New York.

    Google Scholar 

  15. Tung, L. (1993), Report No. LBL-34669, Lawrence Berkeley Laboratory, University of California, Berkely, CA.

    Google Scholar 

  16. Kabawata, N., Yoshida, J., and Tanigawa, Y. (1981) Ind. Eng. Chem. Prod. Res. Dev. 20, 386–390.

    Article  Google Scholar 

  17. Frierman, M., Kuo, Y., Joshi, D., Garcia, A. A., and King, C. J. (1987) Sep. Purif. Methods. 16, 91–102.

    Article  CAS  Google Scholar 

  18. Evangelista, R. L., Nikolov, Z. L., and Mangold, A. J. (1994) Appl. Biochem. Biotech. 45/46, 131–144

    Article  Google Scholar 

  19. Weast, R. C. (1987) Handbook of Chemistry and Physics, CRC, Boca Raton, FL, p. 99.

    Google Scholar 

  20. Kuo, Y., Munson, C. L., Rixey, W. G., Garcia, A. A., Frierman, M., and King, C. J., (1987) Sep. Purif. Methods. 16, 31–64.

    Article  CAS  Google Scholar 

  21. Rohm and Haas (1988) Rohm and Haas Ion Exchange Resin Laboratory Guide, Philadelphia, PA.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1996 Springer Science+Business Media New York

About this chapter

Cite this chapter

Evangelista, R.L., Nikolov, Z.L. (1996). Recovery and Purification of Lactic Acid from Fermentation Broth by Adsorption. In: Wyman, C.E., Davison, B.H. (eds) Seventeenth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium, vol 57/58. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0223-3_43

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-0223-3_43

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6669-3

  • Online ISBN: 978-1-4612-0223-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics