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Saccharification of Native Sugar Cane Bagasse Pith by the Cross-Synergistic Action of Cellulases from Penicillium sp. CH-M-001 and A. Terreus CH-M-013

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Abstract

Filter paper, carboxymethylcellulase, β-glucosidase, and xylanase activities were determined and compared to cellulases originating from Aureobasidium sp. (Ab), Penicillium sp. (Pe), and Aspergillus terreus (At). The formation of total reducing sugar was measured as a function of time for the hydrolysis of the native sugar cane bagasse pith using culture filtrates with the same quantity of extra-cellular protein content from Ab, Pe, At, or from the following mixtures Ab:Pe/ Ab:At and Pe:At at different volumetric ratios. The saccharification progress indicated a synergistic effect for the mixture of all the enzymes, the highest being in the 40:60 relation of Pe and At, respectively. The synergistic action has been assigned to a better balance of endo-and exoglucanases in this system and essentially to the addition of ß-glucosidase and xylanases from At to Pe cellulases

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© 1996 Springer Science+Business Media New York

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Garcia-Kirchner, O., Huitron, C. (1996). Saccharification of Native Sugar Cane Bagasse Pith by the Cross-Synergistic Action of Cellulases from Penicillium sp. CH-M-001 and A. Terreus CH-M-013. In: Wyman, C.E., Davison, B.H. (eds) Seventeenth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium, vol 57/58. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0223-3_23

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  • DOI: https://doi.org/10.1007/978-1-4612-0223-3_23

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6669-3

  • Online ISBN: 978-1-4612-0223-3

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