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Development of High-Performance and Rapid Immunoassay for Model Food Allergen Lysozyme Using Antibody-Conjugated Bacterial Magnetic Particles and Fully Automated System

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Twenty-Second Symposium on Biotechnology for Fuels and Chemicals

Part of the book series: ABAB Symposium ((ABAB))

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Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components inegg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

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Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho Koganei, Tokyo, 184-8588, Japan

    Reiko Sato, Haruko Takeyama, Tsuyoshi Tanaka & Tadashi Matsunaga

Authors
  1. Reiko Sato
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  2. Haruko Takeyama
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  3. Tsuyoshi Tanaka
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  4. Tadashi Matsunaga
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Corresponding author

Correspondence to Tadashi Matsunaga .

Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Brian H. Davison

  2. National Renewable Energy Laboratory, USA

    James McMillan  & Mark Finkelstein  & 

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© 2001 Springer Science+Business Media New York

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Sato, R., Takeyama, H., Tanaka, T., Matsunaga, T. (2001). Development of High-Performance and Rapid Immunoassay for Model Food Allergen Lysozyme Using Antibody-Conjugated Bacterial Magnetic Particles and Fully Automated System. In: Davison, B.H., McMillan, J., Finkelstein, M. (eds) Twenty-Second Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0217-2_9

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  • DOI: https://doi.org/10.1007/978-1-4612-0217-2_9

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6667-9

  • Online ISBN: 978-1-4612-0217-2

  • eBook Packages: Springer Book Archive

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