Effect of Pretreatment Reagent and Hydrogen Peroxide on Enzymatic Hydrolysis of Oak in Percolation Process
The effect of pretreatment reagent and hydrogen peroxide on enzymatic digestibility of oak was investigated to compare pretreatment performance. Pretreatment reagents used were ammonia, sulfuric acid, and water. These solutions were used without or in combination with hydrogen peroxide in the percolation reactor. The reaction was carried out at 170°C for the predetermined reaction time. Ammonia treatment showed the highest delignificationbut the lowest digestibility and hemicellulose removal among the three treatments. Acid treatment proved to be a very effective method in terms of hemicellulose recovery and cellulose digestibility. Hemicelluloserecovery was 65–90% and digestibilities were >90% in the range of 0.01–0.2% acid concentration. In both treatments, hydrogen peroxide had some effect on digestibility but decomposed soluble sugars produced during pretreatment. Unlike ammonia and acid treatments, hydrogen peroxide in water treatment had a certain effect on hemicellulose recovery as well asdelignification. At 1.6% hydrogen peroxide concentration, both hemicellulose recovery and digestibility were about 90%, which were almost the same as those of 0.2% sulfuric acid treatment. Also, digestibility was in vestigatedas a function of hemicellulose removal or delignification. It was found that digestibility was more directly related to hemicellulose removal rather than delignification.
Index EntriesPretreatment ammonia acid water hydrogen peroxide enzymatic hydrolysis
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