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Preliminary Kinetic Characterization of Xylose Reductase and Xylitol Dehydrogenase Extracted from Candida guilliermondii FTI 20037 Cultivated in Sugarcane Bagasse Hydrolysate for Xylitol Production

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Twenty-Second Symposium on Biotechnology for Fuels and Chemicals

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Abstract

Candida guilliermondii FTI 20037 was cultured in sugarcane bagasse hydrolysate supplemented with 2.0 g/L of (NH4)2SO4,0.1 g/L of CaCl2•2H2O, and 20.0 g/L of rice bran at 35°C; pH 4.0; agitation of 300 rpm; and aeration of 0.4, 0.6, or 0.8 vvm. The high xylitol production (20.0 g/L) and xylose reductase (XR) activity (658.8 U/mg of protein) occurred at an aeration of 0.4 vvm. Under this condition, the xylitol dehydrogenase (XD) activity was low. The apparent K M for XR and XD against substrates and cofactors were as follows: for XR,6.4 × 10−2 M (xylose) and 9.5 × 10−3mM (NADPH); for XD, 1.6 × 10−1 M (xylitol) and 9.9 × 10−2mM (NAD+). Because XR requires about 10-fold less xylose and cofactor than XD for the condition in which the reaction rate is half of the V max , some interference on the overall xylitol production by the yeast could be expected.

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Authors and Affiliations

  1. Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmacy, University of São Paulo, Av. Prof. Lineu Prestes, 580, B-16, 05508-900, São Paulo, SP, Brazil

    Luciane Sene & Michele Vitolo

  2. FAENQUIL—DEBIQ-125600-000, PO Box 116, Lorena, SP, Brazil

    Maria G. A. Felipe & Silvio S. Silva

Authors
  1. Luciane Sene
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  2. Maria G. A. Felipe
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  3. Silvio S. Silva
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  4. Michele Vitolo
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Corresponding author

Correspondence to Michele Vitolo .

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Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Brian H. Davison

  2. National Renewable Energy Laboratory, USA

    James McMillan  & Mark Finkelstein  & 

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Sene, L., Felipe, M.G.A., Silva, S.S., Vitolo, M. (2001). Preliminary Kinetic Characterization of Xylose Reductase and Xylitol Dehydrogenase Extracted from Candida guilliermondii FTI 20037 Cultivated in Sugarcane Bagasse Hydrolysate for Xylitol Production. In: Davison, B.H., McMillan, J., Finkelstein, M. (eds) Twenty-Second Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0217-2_56

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  • DOI: https://doi.org/10.1007/978-1-4612-0217-2_56

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6667-9

  • Online ISBN: 978-1-4612-0217-2

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