Influence of Culture Conditions on Lipopeptide Production by Bacillus subtilis

  • Eric Akpa
  • Philippe Jacques
  • Bernard Wathelet
  • Michel Paquot
  • Regine Fuchs
  • Herbert Budzikiewicz
  • Philippe Thonart
Chapter
Part of the ABAB Symposium book series (ABAB)

Abstract

Bacillus subtilis produces various families of lipopeptides with different homologous compounds. To produce “new molecules” with improved activities and to select strains that produced a reduced number of homologs or isomers, we studied the effects of different media on the nature of the synthesis of fatty acid chains for each lipopeptide family. This study focused on two B. subtilis strains cultivated in flasks. Optimized medium for lipopeptide production and Landy medium modified by replacing glutamic acid with other α-amino acids were used. We found that the intensity of production of homologous compounds depends on the strain and the culture medium. Analysis of these lipopeptides by high-performance liquid chromatography showed that the strain B. subtilis NT02 yielded various homologous compounds when cultivated in Landy medium (L-Glu), but primarily one homologous product in high relative amounts when cultivated in the optimized medium. Mass spectrometric analysis and determination of the amino acid composition of this molecule enabled us to identify it as Bacillomycine L cl5.

Index Entries

Bacillus subtilis lipopeptide biosurfactant bacillomycin high-performance liquid chromatography culture medium 

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Eric Akpa
    • 1
  • Philippe Jacques
    • 1
  • Bernard Wathelet
    • 2
  • Michel Paquot
    • 2
  • Regine Fuchs
    • 3
  • Herbert Budzikiewicz
    • 3
  • Philippe Thonart
    • 1
  1. 1.CWBI (Unité de Bio-Industries)Faculté Universitaire des Sciences AgronomiquesGemblouxBelgium
  2. 2.Unité de Chimie BiologiqueFaculté Universitaire des Sciences AgronomiquesGemblouxBelgium
  3. 3.Institut für Organische ChemieKölnGermany

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