Influence of Culture Conditions on Lipopeptide Production by Bacillus subtilis

  • Eric Akpa
  • Philippe Jacques
  • Bernard Wathelet
  • Michel Paquot
  • Regine Fuchs
  • Herbert Budzikiewicz
  • Philippe Thonart
Part of the ABAB Symposium book series (ABAB)


Bacillus subtilis produces various families of lipopeptides with different homologous compounds. To produce “new molecules” with improved activities and to select strains that produced a reduced number of homologs or isomers, we studied the effects of different media on the nature of the synthesis of fatty acid chains for each lipopeptide family. This study focused on two B. subtilis strains cultivated in flasks. Optimized medium for lipopeptide production and Landy medium modified by replacing glutamic acid with other α-amino acids were used. We found that the intensity of production of homologous compounds depends on the strain and the culture medium. Analysis of these lipopeptides by high-performance liquid chromatography showed that the strain B. subtilis NT02 yielded various homologous compounds when cultivated in Landy medium (L-Glu), but primarily one homologous product in high relative amounts when cultivated in the optimized medium. Mass spectrometric analysis and determination of the amino acid composition of this molecule enabled us to identify it as Bacillomycine L cl5.

Index Entries

Bacillus subtilis lipopeptide biosurfactant bacillomycin high-performance liquid chromatography culture medium 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Delcambe, L. (1965), Bull. Soc. Chim. Beiges 74, 329–340.CrossRefGoogle Scholar
  2. 2.
    Arima, K., Kakinuma, A., and Tamura, G. (1968), Biochem. Biophys. Res. Commun. 31(3), 488–494.CrossRefGoogle Scholar
  3. 3.
    Kakinuma, A., Sugino, H., Isono, M., Tamura, G., and Arima, K. (1969), Agric. Biol. Chem. 33(6), 973–976.CrossRefGoogle Scholar
  4. 4.
    Peypoux, F., Michel, G., and Delcambe, L. (1976), Eur. J. Biochem. 63, 391–398.CrossRefGoogle Scholar
  5. 5.
    Vanittanakom, N., Loffler, W., Koch, U., and Jung, G. (1986), J. Antibiot. 39(7), 888–901.CrossRefGoogle Scholar
  6. 6.
    Saotoshi, K. (1988), in Proceedings of the World Conference on Biotechnology for the Fats and Oils Industry, Appelwhite, T. H., ed., American Oil Chemist’s Society, pp. 195–201.Google Scholar
  7. 7.
    Shigeo, I. (1988), in Proceedings of the World Conference on Biotechnology for the Pats and Oils Industry, Appelwhite, T. H., ed., American Oil Chemist’s Society, pp. 206–210.Google Scholar
  8. 8.
    Brown, J. M. and Moses, V. (1988), in Proceedings of the World Conference on Biotechnology for the Fats and Oils Industry, Appelwhite, T. H., ed., American Oil Chemist’s Society, pp. 202–205.Google Scholar
  9. 9.
    Jacques, P., Hbid, C., Vanhentenryck, F., Destain, J., Bare, G., Razafindralambo, H., Paquot, M., and Thonart, P. (1993), Prog. Biotechnol. 3, 1067–1070.Google Scholar
  10. 10.
    Maget-Dana, R. and Peypoux, F. (1994), Toxicology 87, 151–174.CrossRefGoogle Scholar
  11. 11.
    Besson, F., Peypoux, F., Michel, G., and Delcambe, L. (1979), J. Antibiot. 32(8), 828–833.CrossRefGoogle Scholar
  12. 12.
    Besson, F, Peypoux, F., and Michel, G. (1979), Biochimica Biophysica Acta 552, 558–562.CrossRefGoogle Scholar
  13. 13.
    Peypoux, F., Besson, F., Michel, G., and Delcambe, L. (1979), J. Antibiot. 32, 136–140.CrossRefGoogle Scholar
  14. 14.
    Bernheimer, A. W. and Avigad, L. S. (1970), J. Gen. Microbiol. 61, 361–369.Google Scholar
  15. 15.
    Razafindralambo, H., Paquot, M., Baniel, A., Popineau, Y., Hbid, C., Jacques, P., and Thonart, P. (1997), Food Hydrocolloids 11(1), 59–62.CrossRefGoogle Scholar
  16. 16.
    Thimon, L., Peypoux, F., Maget-Dana, G., and Michel, G. (1992), JAOCS 69, 92, 93.CrossRefGoogle Scholar
  17. 17.
    Nissen, E., Pauli, G., Vater, J., and Vollenbroich, D. (1997), In Vitro Cell. Dev. Biol-Anim. 33, 414–415.CrossRefGoogle Scholar
  18. 18.
    Vollenbroich, D., Pauli, G., Vater, J., Ozel, M., and Kamp, R. M. (1997), Biologicals 3, 289–297.Google Scholar
  19. 19.
    Hbid, C. (1996), PhD thesis, Université de Liège.Google Scholar
  20. 20.
    Bland, J., Lax, A., and Klich, M. (1993), Proceedings of the Twenty-Second European Peptide Symposium, Schneider, C. H. and Eberle, A. N., eds., ESCOM, Leiden, The Netherlands, pp. 332–333.Google Scholar
  21. 21.
    Harnois, I., Maget-Dana, R., and Ptak, M. (1989), Biochimie 71, 111–116.CrossRefGoogle Scholar
  22. 22.
    Thimon, L., Peypoux, F., Das, B. C., Wallach, J., and Michel, G. (1994), Biotechnol. Appl. Biochem. 20, 415–423.Google Scholar
  23. 23.
    Jacques, P., Hbid, C., Destain, J., Razafindralambo, H., Paquot, M., De Pauw, E., and Thonart, P. (1999), Appl. Biochem. Biotechnol. 77–79, 223–233.Google Scholar
  24. 24.
    Landy, M., Warren, G. H., Rosenman, S. B., and Colio, L. G. (1948), Proc. Soc. Exp. Biol. Med. 67, 530–541.Google Scholar
  25. 25.
    Razafindralambo, H., Paquot, M., Hbid, C., Jacques, P., Destain, J., and Thonart, P. (1993), J. Chromatogr. 639, 81–85.CrossRefGoogle Scholar
  26. 26.
    Kaneda, T. (1977), Bacteriol. Rev. 41, 391–418.Google Scholar
  27. 27.
    Peypoux, F. and Michel, G. (1992), Appl. Microbiol. Biotechnol. 36, 515–517.Google Scholar
  28. 28.
    Besson, F., Tenoux, I., Hourdou, M.-L., and Michel, G. (1992), Biochimica Biophysica Acta 1123, 51–58.CrossRefGoogle Scholar
  29. 29.
    Besson, F. and Hourdou, M.-L. (1987), J. Antibiot. 60(2), 221–223.Google Scholar
  30. 30.
    Hourdou, M.-L. (1989), PhD thesis, Universite Claude Bernard-Lyon 1, France.Google Scholar
  31. 31.
    Hourdou, M.-L., Besson, F., and Michel, G. (1988), J. Antibiot. 61(2), 207–211.Google Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Eric Akpa
    • 1
  • Philippe Jacques
    • 1
  • Bernard Wathelet
    • 2
  • Michel Paquot
    • 2
  • Regine Fuchs
    • 3
  • Herbert Budzikiewicz
    • 3
  • Philippe Thonart
    • 1
  1. 1.CWBI (Unité de Bio-Industries)Faculté Universitaire des Sciences AgronomiquesGemblouxBelgium
  2. 2.Unité de Chimie BiologiqueFaculté Universitaire des Sciences AgronomiquesGemblouxBelgium
  3. 3.Institut für Organische ChemieKölnGermany

Personalised recommendations