Abstract
Potentially powerful tools for treatment of acquired and inherited diseases of the myocardium are the introduction of replacement genes or targeted disruption of diseased proteins. Direct intramuscular injection (Ascadi et al., 1991) and viral vectors (Dunckley et al., 1992) have been previously used for the transfer of functional cDNA constructs into muscle cells, but these are dependent on possible low expression or negative responses to the viral vector. A promising alternative to these methods that currently is being extensively studied is the use of cationic liposomes. Benefits of cationic liposomes include the ability to protect nucleic acids from degradation and the efficient delivery of tagged oligonucleotides to the nucleus (Zelphati and Szoka, 1996). In this chapter, we describe different strategies for using cationic liposomes to analyze cardiac myofibril organization and formation in whole intact hearts. Results from these applications have yielded valuable information on myofibril initiation and segmental heart development.
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Zajdel, R.W., McLean, M.D., Denz, C.R., Dube, S., Lemanski, L.F., Dube, D.K. (2002). Manipulation of Myofibrillogenesis in Whole Hearts. In: Dube, D.K. (eds) Myofibrillogenesis. Cardiovascular Molecular Morphogenesis. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0199-1_6
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DOI: https://doi.org/10.1007/978-1-4612-0199-1_6
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