Effect of kLa on the Production of Glucose 6-Phosphate Dehydrogenase from Saccharomyces cerevisiae Grown by Fermentation Process

  • Daniel Pereira Silva
  • Adalberto PessoaJr.
  • Inês C. Roberto
  • Michele Vitolo
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

In a 5-L fermentor (NBS-MF105), Saccharomyces cerevisiae (0.7 g/L) was inoculated into a liquid medium (pH 4.0) containing 17 g/L of glucose, 2.55 g/L of yeast extract, 4.25 g/L of peptone, 2.04 g/L of Na2HP0412H2O, 4.34 g/L of (NH4)2SO4 and 0.064 g/L of MgSO4 7H2O and aerobically cultivated at 35°C for 22 h. Agitation and aeration were adjusted to attain initial k L a values of 15, 60,135, and 230 h-1. The glucose 6-phosphate dehydrogenase (G6PDH) productivity (Pr G6PDH) obtained for kLa values of 15, 60, 135, and 230 h-1 was 10.6, 31.8, 30.3, and 23.3 U/([L-h]), respectively, whereas the cell productivity (Pr X) for the same k L a values were 0.24,0.69,0.69, and 0.49 g/[L h], respectively. Thus, both events are coupled and depend on the dissolved oxygen in the medium.

Index Entries

Glucose 6-phosphate dehydrogenase Saccharomyces cerevisiae Fermentation volumetric coefficient of oxygen transfer 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Daniel Pereira Silva
    • 1
  • Adalberto PessoaJr.
    • 1
  • Inês C. Roberto
    • 2
  • Michele Vitolo
    • 1
  1. 1.Department of Biochemical and Pharmaceutical Technology, Faculty of PharmacyUniversity of São PauloSão PauloBrazil
  2. 2.DEBIQ-FAENQUILLorenaBrazil

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