Abstract
An ammonia process was applied at several ammonia loadings, moisture contents, temperatures, and dwell times. A cellulase loading of 5 FPU/g dry matter and a 24 h incubation time were used to produce the sugars, which were measured as reducing sugars and by HPLC. Optimal processing conditions caused a 76% of theoretical yield (2.9-fold above untreated). Cellulose and hemicellulose conversions were 68 and 85% (vs 38 and 34% in untreated, respectively). The short hydrolysis time and relatively low enzyme loading suggests great potential to produce sugars from alfalfa.
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Ferrer, A., Byers, F.M., Sulbarán-De-Ferrer, B., Dale, B.E., Aiello, C. (2002). Optimizing Ammonia Processing Conditions to Enhance Susceptibility of Legumes to Fiber Hydrolysis. In: Finkelstein, M., McMillan, J.D., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0119-9_10
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DOI: https://doi.org/10.1007/978-1-4612-0119-9_10
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