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Adsorption of Components of Enzymatic Synthesis of Ampicillin on Different Hydrophobic Resins

  • Marcelo F. Vieira
  • Marlei Barboza
  • Raquel Lima C. de Giordano
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

This work compared the performance of three hydrophobic resins for the adsorption of ampicillin (AMP), d-phenylglycine (PG), d-phenylglycine methyl ester (PGME), and 6- aminopenicillanic acid (6-APA). The influence of pH on adsorption efficiencies was assessed in the range of 4.5–8.5, at 4 and 25°C. The values at 4°C were slightly higher than those at 25°C. The adsorption efficiency of AMP and 6-APA decreased at higher pHs, for the three resins. An opposite behavior was found for PGME, and the pH did not affect PG adsorption efficiency. Isotherm models were fitted to experimental equilibrium data and the best models were discriminated.

Index Entries

Ampicillin purification adsorption β-lactamics 

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References

  1. 1.
    Ospina, S., Barzana, E., Ramírez, O., Thoa, T., and López-Munguía, A. (1996), Enzyme Microb. Technol. 19, 462–469.CrossRefGoogle Scholar
  2. 2.
    Hernández-Jústiz, O., Terreni, M., Pagani, G., García, J. L., Guisán, J. M., Fernández-Lafuente, R. (1999), Enzyme Microb. Technol. 25, 336–343.CrossRefGoogle Scholar
  3. 3.
    Wheelwright, S. M. (1987), Bio/Technology 5(8), 789.CrossRefGoogle Scholar
  4. 4.
    Doulia, D., Rigas, F., and Gimouhopoulos, C. (2001), J. Chem. Technol. Biotechnol. 76, 83–89.CrossRefGoogle Scholar
  5. 5.
    Kirkby, N. F., Slater, N. K. H., Weisengerger, K. H.; Addo-Yobo, F. and Doulia, D. (1986), Chem. Eng. Sci. 41(8), 2005–2016.CrossRefGoogle Scholar
  6. 6.
    Casillas, J. L., Martinez, M., Addo-Yobo, F., Aracil, J. (1993), Chem. Eng. J. Biochem. Eng. 52(3), B71–B75.CrossRefGoogle Scholar
  7. 7.
    Grzegorczyk S. and Carta, G. (1996), Chem. Eng. Sci. 51, 819–826.CrossRefGoogle Scholar
  8. 8.
    Chaubal, M. V., Payne, F. G., Reynolds, C. H., and Albright, R. L. (1995), Biotech. Bioeng. 47, 215–226.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Marcelo F. Vieira
    • 1
  • Marlei Barboza
    • 2
  • Raquel Lima C. de Giordano
    • 1
  1. 1.Departamento de Engenharia QuímicaUniversidade Federal de São CarlosSão CarlosBrazil
  2. 2.Departamento de Engenharia Química, UNAERPUniversidade de Ribeirão PretoRibeirão PretoBrazil

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