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Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety and Efficacy Evaluation

  • Phuc Van Pham
  • Khanh Hong Thien Bui
  • Dat Quoc Ngo
  • Thao Thi-Phuong Doan
  • Ngoc Bich Vu
  • Nhung Hai Truong
  • Dung Minh Le
  • Ngoc Kim Phan
Chapter

Abstract

Non-expanded adipose tissue-derived stem cells (ADSCs) are commonly used in preclinical and clinical articular cartilage injury treatment; however, there are usually insufficient non-expanded ADSCs for transplantation. This research aims to evaluate the safety and efficacy of expanded ADSC transplantation in a mouse model. The stromal vascular fraction from abdominal adipose tissue was subcultured for ten passages to enrich for and expand the number of ADSCs. The safety of expanded ADSCs was assessed by evaluating their “stemness” via ADSC-specific marker expression, the expression of two tumorigenesis-related genes (Oct-3/4 and Nanog) using real-time RT-PCR, and in vivo tumor formation in NOD/SCID mice. ADSC efficacy was determined by assessing their chondrocytic differentiative potential in vitro in cartilage-inducing medium as well as in vivo via injection into a NOD/SCID mice joint failure model. The results showed that expanded ADSCs were negative for Oct-3/4 and Nanog and did not induce tumor formation in mice. Furthermore, ADSCs differentiated into chondrocytes, both in vitro and in vivo, and enhanced the regeneration of articular cartilage in NOD/SCID mice as compared with the control. These results confirm that expanded ADSCs are safe and effective for the treatment of injured articular cartilage and offer a promising therapy for degenerative cartilaginous diseases.

Keywords

Articular Cartilage Graft Versus Host Disease Stromal Vascular Fraction Fetal Bovine Serum Medium Stromal Vascular Fraction Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Conflicts of Interest

The authors have no conflicts of interest.

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Copyright information

© Springer-Verlag London 2015

Authors and Affiliations

  • Phuc Van Pham
    • 1
  • Khanh Hong Thien Bui
    • 2
  • Dat Quoc Ngo
    • 3
  • Thao Thi-Phuong Doan
    • 3
  • Ngoc Bich Vu
    • 1
  • Nhung Hai Truong
    • 1
  • Dung Minh Le
    • 1
  • Ngoc Kim Phan
    • 1
  1. 1.Laboratory of Stem Cell Research and ApplicationUniversity of Science¸ Vietnam National University, Ho Chi Minh CityHo Chi Minh CityVietnam
  2. 2.Orthopaedic DepartmentUniversity Medical Center, Ho Chi Minh City, University of Medicine and Pharmacology, Ho Chi Minh CityHo Chi Minh CityVietnam
  3. 3.Department of PathologyUniversity of Medicine and Pharmacy, Ho Chi Minh CityHo Chi Minh CityVietnam

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