Abstract
In the following study we report on the development and application of the cremaster chamber model. Seventeen male Sprague-Dawley rats were studied in two experimental groups. In the control group, Group I, the muscle flap was preserved in the medial border of the rat’s hind limb. For evaluation the cremaster was removed from the leg and prepared for in vivo observations after 24 h. In Group II, the cremaster muscle chamber was implanted and in vivo observations were performed in post-operative intervals of half an hour, 24, 48 and 72 h, respectively. The study proved that the cremaster chamber model was equal to the classic cremaster muscle preparation for microcirculatory measurements for at least 24 h.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Sandison JC. The transparent chamber of the rabbit’s ear giving a complete description of improved techniques of construction and introduction and general account of growth and behavior of living cells and tissues as seen with the microscope. Am J Anat. 1928;41:447.
Algire GH. An adaptation of the transparent chamber technique to the mouse. J Natl Cancer Inst. 1943;4:1.
Lehr H, Leunig M, Menger MD, Nolte D, Messmer K. Technical advances. Dorsal skinfold chamber technique for intravital microscopy in nude mice. Am J Pathol. 1993;143:1055.
Nolte D, Schmid P, Jäger U, Botzlar A, Roesken F, Hecht R, Uhl E, Messmer K, VestWeber D. Leukocyte rolling in venules of striated muscle and skin is mediated by P-selectin, not by L-selectin. Am J Physiol. 1994;267:H1637. Heart Circ Physiol 36.
Greenblatt M, Shubik P. Hamster cheek pouch chamber. Cancer Bull. 1967;19:65.
Yasuhara H, Hobson II RW, Dillon PK, Duran WN. A new model for studying ischemia-reperfusion injury in hamster cheek pouch. Am J Physiol. 1991;261:H1626. Heart Circ Physiol 30.
Yamaura H, Suzuki M, Sato H. Transparent chamber in the rat skin for studies on microcirculation in cancer tissue. Gann. 1971;62:177.
Hobbs JB, Chusilp S, Hua A, Kincaid-Smith P, Mclver MA. The pathogenesis of hypertensive vascular changes in the rat: microscopic and ultrastructural correlation in vivo. Clin Sci. 1976;51:71s.
Smith TL, Curl WW, Smith BP, Holden MB, Wise T, Marr A, Koman A. New skeletal muscle model for the longitudinal study of alterations in microcirculation following contusion and cryotherapy. Microsurgery. 1993;14:487.
Hori K, Zhang Q, Saito S, Tanda S, Li H, Suzuki M. Microvascular mechanisms of change in tumor blood flow due to angiotensin II, epinephrine, and methoxamine: a functional morphometric study. Cancer Res. 1993;53:5528.
Arfors KE, Jonsson JA, McKensie FN. A titanium rabbit ear chamber: assembly, insertion, and results. Microvasc Res. 1970;2:516.
Clark ER, Kirby-Smith HT, Rex RO, Williams RG. Recent modifications in the method of studying living cells and tissues in transparent chambers inserted in the rabbit’s ear. Anat Rec. 1930;47:187.
Brennan SS, Leaper DJ. The effect of antiseptics on the healing wound: a study using the rabbit ear chamber. Br J Surg. 1985;72:780.
Suzuki Y, Suzuki K, Ishikawa K. Direct monitoring of the microcirculation in experimental venous flaps with afferent arteriovenous fistulas. Br J Plast Surg. 1994;47:554.
BrĂ¥nemark PL, Aspegren K, Breine U. Microcirculatory studies in man by high resolution vital microscopy. Angiology. 1964;15:329.
Cardon SZ, Ostermeyer CF, Bloch EH. Effect of oxygen on red blood cell flow in unanesthetized mammalian striated muscle as determined by microscopy. Microvasc Res. 1970;2:67.
Endrich B, Asaishi K, Goetz A, Messmer K. Technical report-a new chamber technique for microvascular studies in unasthethized hamsters. Res Exp Med. 1980;177:125.
Papenfuss HD, Gross JF, Intaglietta M, Treese FA. A transparent access chamber for the rat dorsal skin fold. Microvasc Res. 1979;18:311.
Falkvoll KH, Rofstad EK, Brustad T, Marton P. A transparent chamber for the skin fold of athymic mice. Exp Cell Biol. 1984;52:260.
Anderson T, Acland RD, Siemionow M, McCabe SJ. Vascular isolation of the rat cremaster. Microvasc Res. 1988;36:56.
Acland RD, Anderson G, Siemionow M, Mc Cabe S. Direct in vivo observations of embolic events in the microcirculation distal to a small-vessel anastomosis. Plast Reconstr Surg. 1989;84:280–8.
Siemionow M, Anderson T, Lister G. Hemodynamic variations between end- to- side and end-organ flap systems. J Hand Surg Am. 1995;20A:205–12.
Franken RJ, Peter FW, Anderson GL, et al. Anatomy of the feeding blood vessels of the cremaster muscle in the rat. Microsurgery. 1996;17(7):402–8.
Nanhekhan LV, Siemionow M. Microcirculatory hemodynamics of the rat cremaster muscle flap in reduced blood flow states. Ann Plast Surg. 2003;51:182–8.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag London
About this chapter
Cite this chapter
Nanhekhan, L. (2015). Cremaster Chamber Model. In: Siemionow, M. (eds) Plastic and Reconstructive Surgery. Springer, London. https://doi.org/10.1007/978-1-4471-6335-0_15
Download citation
DOI: https://doi.org/10.1007/978-1-4471-6335-0_15
Published:
Publisher Name: Springer, London
Print ISBN: 978-1-4471-6334-3
Online ISBN: 978-1-4471-6335-0
eBook Packages: MedicineMedicine (R0)