Cana Cloning and Plant Expression Vector Construction of Soybean 11s Glycerin Gy3

Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 217)

Abstract

The RNA was extracted from immature seeds of soybean and it is directly used in single-stranded dank synthetic reaction. According to soybean 11s glycerin Gy3 coda sequences in gene bank, a pair of primers was designed. Double-stranded coda was amplified by PCR. The amplified Gy3 coda was cloned into plasmid pMD19-T and sequenced. Fragment of the Gy3 coda in the recombinant plasmid was cut with Xbox I/bah I and ligated with plasmid pbi121, and then a plant expression vector was constructed. It includes camv35 proctor, nose terminator and no-II gene.

Keywords

Soybean Gy3 Coda Clone Plant expression vector 

References

  1. 1.
    Zheng Y, Mengyuan H (1992) Soybean cotyledon protein body formation and storage protein accumulation in the relationship. Act Bot Sinica 1 (3):41–45Google Scholar
  2. 2.
    Kats be T, Korimako N, Ogawa M (1999) Accumulation of soybean glycerin and its assembly with the glutei’s in rice. Plant Physiol 2(12):1070–1074Google Scholar
  3. 3.
    Xian in Z, Qing zhong X (2001) With agro bacterium mediating method of soybean glycerin gene into rice. J Zhejiang Univ 3(12):495–499Google Scholar
  4. 4.
    Jianzhong T, Cheng Fu L, Ming Z et al (1999) Soybean glycerin gene expression vector and the genetic transformation of mulberry silkworm. Course Study Sci 4(13):5–9Google Scholar
  5. 5.
    Utsumi S, Kohno M, Mori T, Kato M (1997) An alternate coda encoding glycerin A-1a-B-x subunit. Agric Food Chem 5(8):210–214Google Scholar
  6. 6.
    Beilinson V, Chen Z et al (2002) Genomic organization of glycinin genes in soybean. Theory Apple Genet 6(7):1132–1140Google Scholar
  7. 7.
    Cretan PB, Kollipara KP (2001) Primary trisomics and SSR markers as tools to associate chromosomes with linkage groups in soybeans. Crop Sci 7(21):1262–1267Google Scholar
  8. 8.
    Momma K, Hashimoto W, Ozawa S (1999) Teal Quality and safety evaluation of genetically engineered rice with soybean glycerin: analyses of the grain composition and digestibility of glycerin in transgenic rice. Basic Biotechnol Brioche 8(6):314–318Google Scholar
  9. 9.
    Mink DW, Chen J (1981) Beach Expression of Storage Protein Genes during Soybean Seed Development Plantar. Adv Modern Gene 9(15):1302–1304Google Scholar
  10. 10.
    Dickinson CD, Hussein EHA, Nielsen NC (1989) Role of post-translational cleavage in glycerin assembly. J Bacteriol 10(9):459–469Google Scholar

Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  1. 1.Tangshan CollegeTangshanChina

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