Cana Cloning and Plant Expression Vector Construction of Soybean 11s Glycerin Gy3

Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 217)


The RNA was extracted from immature seeds of soybean and it is directly used in single-stranded dank synthetic reaction. According to soybean 11s glycerin Gy3 coda sequences in gene bank, a pair of primers was designed. Double-stranded coda was amplified by PCR. The amplified Gy3 coda was cloned into plasmid pMD19-T and sequenced. Fragment of the Gy3 coda in the recombinant plasmid was cut with Xbox I/bah I and ligated with plasmid pbi121, and then a plant expression vector was constructed. It includes camv35 proctor, nose terminator and no-II gene.


Soybean Gy3 Coda Clone Plant expression vector 


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  1. 1.Tangshan CollegeTangshanChina

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