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Avidin-Biotin Immobilisation Systems

  • M. Wilchek
  • E. A. Bayer
Part of the Springer Series in Applied Biology book series (SSAPPL.BIOLOGY)

Abstract

The avidin-biotin system has become an extremely useful tool for many different applications in the fields of biology, biochemistry and biotechnology. The unusual affinity (Kd 10-15 M) of avidin (or its bacterial relative, streptavidin) for the vitamin biotin forms the basis for using this system; the avidin-biotin complex serves as a general mediator which brings together a desired target within a biological system and an external probe. This approach has found broad application, mainly for localisation studies, medical diagnostics and recombinant genetics. The principles and scope of avidin-biotin technology have been extensively described and catalogued, and the reader is referred to the series of reviews which are available in the literature (Bayer and Wilchek 1978, 1980; Bayer et al. 1979; Wilchek and Bayer 1984, 1988, 1989a) as well as a book which has recently appeared (Wilchek and Bayer 1990a).

Keywords

Guanidinium Hydrochloride Guanidinium Isothiocyanate Biotin Moiety Nonglycosylated Form Avidin Molecule 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag London 1993

Authors and Affiliations

  • M. Wilchek
  • E. A. Bayer

There are no affiliations available

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