Avidin-Biotin Immobilisation Systems
The avidin-biotin system has become an extremely useful tool for many different applications in the fields of biology, biochemistry and biotechnology. The unusual affinity (Kd 10-15 M) of avidin (or its bacterial relative, streptavidin) for the vitamin biotin forms the basis for using this system; the avidin-biotin complex serves as a general mediator which brings together a desired target within a biological system and an external probe. This approach has found broad application, mainly for localisation studies, medical diagnostics and recombinant genetics. The principles and scope of avidin-biotin technology have been extensively described and catalogued, and the reader is referred to the series of reviews which are available in the literature (Bayer and Wilchek 1978, 1980; Bayer et al. 1979; Wilchek and Bayer 1984, 1988, 1989a) as well as a book which has recently appeared (Wilchek and Bayer 1990a).
KeywordsEstrogen Polysaccharide Lysine Angiotensin Dexamethasone
Unable to display preview. Download preview PDF.
- Bayer EA, Wilchek M (1978) The avidin-biotin complex as a tool in molecular biology. Trends Biochem Sci 3:N237–N239Google Scholar
- Ruoslahti E (1990) Versatile mechanisms of cell adhesion. The Harvey Lectures, Series 84, 1–17Google Scholar
- Wilchek M, Bayer EA (1989b) A universal affinity column using avidin-biotin technology. In: Hutchens TW (ed) Protein recognition of immobilised ligands. Alan R Liss, Inc New York, pp 83–90Google Scholar
- Wilchek M, Bayer EA (1990a) Avidin-biotin technology (Methods Enzymol, vol 184) Academic Press, OrlandoGoogle Scholar
- Wilchek M, Miron T (1982) Immobilisation of enzymes and affinity ligands onto agarose via stable and uncharged carbamate linkages. Biochem Internat 4:629–635Google Scholar