Avidin-Biotin Immobilisation Systems
The avidin-biotin system has become an extremely useful tool for many different applications in the fields of biology, biochemistry and biotechnology. The unusual affinity (Kd 10-15 M) of avidin (or its bacterial relative, streptavidin) for the vitamin biotin forms the basis for using this system; the avidin-biotin complex serves as a general mediator which brings together a desired target within a biological system and an external probe. This approach has found broad application, mainly for localisation studies, medical diagnostics and recombinant genetics. The principles and scope of avidin-biotin technology have been extensively described and catalogued, and the reader is referred to the series of reviews which are available in the literature (Bayer and Wilchek 1978, 1980; Bayer et al. 1979; Wilchek and Bayer 1984, 1988, 1989a) as well as a book which has recently appeared (Wilchek and Bayer 1990a).
KeywordsGuanidinium Hydrochloride Guanidinium Isothiocyanate Biotin Moiety Nonglycosylated Form Avidin Molecule
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