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Serological Variation in the Fibronectin Binding to Protein-A-Deficient Mutants of Staphylococcus aureus

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Pathogenesis of Wound and Biomaterial-Associated Infections

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Summary

Blocking of 125I-fibronectin (Fn) binding to two protein-A-defi-cient mutants U-320 and U-305 derived from Staphylococcus aureus SA113(83A) was tested, using various human and murine sera. Normal human sera completely inhibited Fn binding to both the mutants. However, sera obtained from patients suffering from acute staphylococcal infections, caused a high degree of inhibition of Fn binding only to U-320, whereas the binding to U-305 was less affected. Also, murine antisera against whole cells of Staphylococcus aureus laboratory strains, Cowan 1 and Newman, or to cloned Fn binding protein (FnBP) blocked the binding of 125I-Fn to U-320 but not to U-305. The Fn binding components of the two mutants showed marked differences in the susceptibility to proteolytic digestion. Our data suggest the existence of more than one type of FnBP in Staphylococcus aureus. The difference in the blocking of 125I-Fn binding to mutants U-320 and U-305 suggests antigenic variation among staphylococcal FnBP.

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Authors
  1. A. Satyanarayan Naidu
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  2. Claes Schalén
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  3. Jan-Ingmar Flock
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  4. Ingrid Nilsson
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  5. Jacek Miedzobrodzki
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  6. Torkel Wadström
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Editor information

Editors and Affiliations

  1. Department of Medical Microbiology, University of Lund, POB 117, S-22100, Lund, Sweden

    Torkel Wadström PhD (Professor) & Åsa Ljungh PhD (Assistant Professor) (Professor) &  (Assistant Professor)

  2. Department Medical Microbiology, Sölvegaten 23, S-23362, Lund, Sweden

    Ingvar Eliasson PhD

  3. Burns Institute, Shriners Hospital, Cincinnati, Ohio, USA

    Ian Holder PhD

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© 1990 Springer-Verlag London Limited

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Naidu, A.S., Schalén, C., Flock, JI., Nilsson, I., Miedzobrodzki, J., Wadström, T. (1990). Serological Variation in the Fibronectin Binding to Protein-A-Deficient Mutants of Staphylococcus aureus . In: Wadström, T., Eliasson, I., Holder, I., Ljungh, Å. (eds) Pathogenesis of Wound and Biomaterial-Associated Infections. Springer, London. https://doi.org/10.1007/978-1-4471-3454-1_43

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  • DOI: https://doi.org/10.1007/978-1-4471-3454-1_43

  • Publisher Name: Springer, London

  • Print ISBN: 978-3-540-19596-2

  • Online ISBN: 978-1-4471-3454-1

  • eBook Packages: Springer Book Archive

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